Cytotoxic Effect of Aqueous Extract of Anastatica hierochuntica
L. on AMN-3 Cell Line in vitro

Taghreed Mohammd

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Publication Date

Sat Jun 06 2015

Journal Name

Advances In Life Science And Technology

Volume

61

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Cytotoxic Effect of Aqueous Extract of Anastatica hierochuntica L. on AMN-3 Cell Line in vitro

: Anastatica hierochuntica L.

Cytotoxic activity

Taghreed Mohammd

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Anastatica hierochuntica L. is distributed throughout Arabain Peninsula, and elsewhere it is locally called "Kuffe Maryam" .All parts of the plant are used in folk medicine. This study amid to investigate the effect of aqueous extract of anastatica hierochunctica L. on the cancer cell lines AMN-3. Anti cancer activity of aqueous extract of anastatica hierochunctica L. showed anticancer activity against AMN-3 cell line for twelve concentrations (0.04, 0.09, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100) mg/mL in comparison with negative control.

Publication Date

Mon Apr 06 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUE

This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)% gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)% gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog

1962 (MS) medium supplemented with 2iP 3mg/L and containing 0

25

50 or 100 mg/L of NAA

the addition (50) mg/L of NAA gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value (18.8)%

as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%

Primary callus was transferred to fresh MS medium containing (0

5

10 and 20) mg/L of NAA with 3mg/L 2iP

Results were recorded after 12 weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks

maximum values were reached in the eighth week of PCV and fresh weight but the dry weight reached to highest value in seventh week

then the curve began to decline gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100

500

10000

50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.

Ali A.

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Publication Date

Tue Jan 01 2019

Journal Name

Journal Of Advanced Research In Dynamical And Control Systems

The impact of node density over routing protocols in manet by using NS-3

Mohammed

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(5)

Publication Date

Thu Nov 02 2023

Journal Name

Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )

Role of Human β-Defensin-3 in Rheumatoid Arthritis: An Observational Single-Center Study

Noor Ulhuda Ghazi

Faiq Isho

Fayhaa Muqdad

Baydaa Ahmed

Samara Ali

Layla Othman

Isam Noori

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Background: It's believed that HBD-3 is involved in the tissue remodeling process of articular cartilage. Also, HBD-3 has anti-inflammatory properties. Objectives: The purpose of this study is to assay human beta-defensine-3 (HBD-3) in serum from rheumatoid arthritis (RA) patients and investigate its correlation with proinflammatory cytokines. Methods: In this case-control study, fifty-eight RA patients were aged 20–65 years, and 29 age-matched healthy subjects (HS) had no inflammatory rheumatic diseases. The disease activity score-28 joint erythrocyte sedimentation rate (DAS28-ESR) was used to measure RA activity. CRP, ACPA, HBD-3, TNF-α, and IL-1β were assessed using the enzyme-linked immunosorbent assay technique (ELISA). Res

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(7)

(1)

Publication Date

Tue Jan 01 2019

Journal Name

The Turkish Journal Of Endocrinology And Metabolism

Cell-free DNA as a Clinical Indicator in Maternal Blood

Al-Omary H.L.

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As many expensive and invasive procedures are used for the diagnosis or follow-up of clinical conditions, the measurement of cell-free DNA is a promising, noninvasive method, which considers using blood, follicular fluid, or seminal fluid. This method is used to determine chromosomal abnormalities, genetic disorders, and indicators of some diseases such as polycystic ovary syndrome, pre-eclampsia, and some malignancies. Cell-free DNA, which are DNA fragments outside the nucleus, originates from an apoptotic process. However, to be used as a marker for the previously mentioned diseases is still under investigation. We discuss some aspects of using cell-free DNA measurements as an indicator or marker for pathological conditions.

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(1)

Publication Date

Sat Oct 01 2016

Journal Name

World Journal Of Pharmaceutical Research

TERATOGENIC EFFECT OF METHOTREXATE ON HISTOGENESIS OF TESTIS IN NEWBORN RAT

Mohammad A.

Nahla

Mohammad Oda

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Background: Methotrexate (MTX) was one of the first drugs synthesized for a specific chemotherapeutic purpose used to inhibit folic acid (FA) for the treatment of acute lymphoblastic leukemia in children. Its history is closely related to the discovery and characterization of folic acid. It is used clinically in medicine to treat a range of cancerous and noncancerous conditions. MTX is currently used in gynecology to treat disorders arising from trophoblastic tissue, namely, ectopic pregnancy. MTX, the most frequently used diseasemodifying anti-rheumatic drug (DMARD), suppresses disease activity and reduces joint damage. The aim of study: It is designed to demonstrate the effect of MTX (7.5 mg/wk.) on the histogenesis of gonad (testis) of n

Publication Date

Sun Sep 03 2017

Journal Name

Baghdad Science Journal

Kinetic and Mechanistic Studies for the Gas-phase Reaction of Ozone with 2, 3-Dimethyl-2-Butene and 1, 3-Butadiene

Ozone

reactions kinetics

absolute rate

relative rate

rate constant

reactivity

and reaction mechanism

Ismael Abdulsatar AL

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The reactions of ozone with 2,3-Dimethyl-2-Butene (CH3)2C=C(CH3)2 and 1,3-Butadiene CH2=CHCH=CH2 have been investigated under atmospheric conditions at 298±3K in air using both relative and absolute rate techniques, and the measured rate coefficients are found to be in good agreement in both techniques used. The obtained results show the addition of ozone to the double bond in these compounds and how it acts as function of the methyl group substituent situated on the double bond. The yields of all the main products have been determined using FTIR and GC-FID and the product studies of these reactions establish a very good idea for the decomposition pathways for the primary formed compounds (ozonides) and give a good information for the effe

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Publication Date

Fri Feb 23 2018

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

Phytochemical and Pharmacological Study of Valepotriates in Valeriana officinalis L. F.Valerianeceae Cultivated in Iraq

Zeina

Kawkab

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This study concerned with phytochemical investigation and methods of extraction and separation of active constituents from Valeriana officinalis plant cultivated in Iraq. Due to the large number of active constituents in Valeriana officinalis, it was necessary to make analytical study of its constituents to determine the chemical nature of these constituents and then determine the main classes (terpenes and iridoids) using chemical reagents specific for each class. Different organic solvents like ethanol (70%) used in soxhlet apparatus and hexane, ethyl acetate and methanol were used separately to extract the main active constituents by maceration. Through comparison between these solvents using thin layer chromatograph

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Publication Date

Tue Jun 30 2020

Journal Name

Journal Of Planner And Development

Real estate acquisition barrier and its impact on urban development projects" Study Area: Infrastructure project in the capital Baghdad (North East Sewage Line project AL khansaa)

Real estate

urban development

acquisition

Infrastructure

Property

Jalal Atheeb

Areaj Khairi

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The increase the rates of natural growth, urbanization and continuous migration, this has generated constant pressure and, as a result, the capital city of Baghdad faces a number of challenges related to its urban environment, including the challenge of acquiring real estate.

and this research examines the impact of these holdings, representing the main base from which the various projects originate Urban in all areas (economic, social, and recreational).

this leads us to the research problem of the obstacles that arise during the process of acquiring real estate to carry out development projects, and to achieve the objectives of research, namely (work to create a regulatory methodology

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Publication Date

Tue Jan 01 2019

Journal Name

Malaysian Journal Of Biochemistry & Molecular Biology

Evaluation influence of extract of Terfezia claveryi desert truffle, pezizaceae, against Streptococcus pneumoniae, in vivo

Hala M.

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(1)

Publication Date

Tue Jan 01 2019

Journal Name

Iop

Water cut comparison for a really similar gridding system (line drive pattern)

Al-Joumaa J.H.

M S

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