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Isolation and Identification of Flavonoid Compounds from Euphorbia Milii Plant Cultivated in Iraq and Evaluation of its Genetic Effects on Two Types of Cancer Cell Line
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يعتبر "تاج الأشواك" أو نبات شوكة المسيح، وهو من نباتات الزينة الطبية ، ينتمي إلى جنس يوفوربيا. E. milii يحتوي كميات وفيرة من المركبات الفينولية ، التربينات، الستيرويدات والقلويدات. كانت الأهداف الرئيسية لهذه الدراسة هي فحص مستخلصات الفلافونويد والنانو فلافونويد ضد نوعين من خطوط الخلايا السرطانية. تم تصنيع مركبات الفلافونويد النانوية عن طريق تفاعل مركب الكيتوسان والماليك اسد. تم تحليل مركبات الفلافونويد النانوية بواسطة مقياس الطيف الضوئي UV-sp -8001 بطول موجي 200-1000 نانومتر ، تم استخدام المجهر الإلكتروني (TEM) والمجهري الإلكتروني الماسح(SEM) لتحديد الخصائص المورفولوجية لمركبات الفلافونويد النانوية. يعتبر الفلافونويد والنانوفلافونويدعقار قوي ومتطور ضد خلايا سرطان الثدي (MCF-7) وخلايا سرطان البروستات(PC3) . تم فحص الفعالية المضادة للسرطان من مركبات الفلافونويد والنانوفلافونويد على خطين مختلفين من الخلايا السرطانية وكذلك خطوط الخلايا السليمة باستخدام اختبار MTT. تم استخدام تجزئة الحمض النووي وتقنية تلوين الخلايا بالصبغة المزدوجة AO / EtBr  لفحص علامات موت الخلايا المبرمج. لتحديد تشتت دورة الخلية ، تم استخدام قياس التدفق الخلوي ، وأظهرت النتائج  أن كميات الفلافونويد والنانوفلافونويد لها نشاطا ساما وفعالا ضد خطوط خلايا سرطان الثدي والبروستات. بالإضافة إلى ذلك ، تم ربط إيقاف دورة الخلية في مرحلة G0 / G1 بموت الخلايا المبرمج لخطوط الخلايا التي يسببها الفلافونويد والنانوفلافونويد. وفقًا لهذه النتائج ، تمنع مركبات الفلافونويد والنانوفلافونويد من تكاثر خطوط الخلايا السرطانية ، مما يؤدي إلى توقف دورة الخلية وتحفيز موت الخلايا المبرمج. تشير النتائج المتاحة إلى أن مركب الفلافونويد والنانوفلافونويد سيمثل نهجًا علاجيًا واعدًا لعلاج الخلايا السرطانية من الأنواع الأخرى.

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Publication Date
Tue Jan 01 2019
Journal Name
In Indian Journal Of Public Health Research And Development
The Significance of p53, Bcl-2, and HER-2/neu Protein Expression in Iraqi Females Breast Cancer Cell Line (AMJ13)
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Publication Date
Sat Jul 01 2023
Journal Name
Iraqi Journal Of Veterinary Sciences
Genetic confirmation for morphological identification of Stilesia globipunctata in camel in Iraq
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Publication Date
Thu Dec 01 2022
Journal Name
Journal Of University Of Anbar For Pure Science
Isolation and identification of the chemical composition of Salvia Species growing in Iraq by GC-MS
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Publication Date
Wed Jan 01 2020
Journal Name
International Journal Of Pharmaceutical Research
Characters anatomy of CorchorusolitoriusL. from Malvaceae family cultivated in Iraq
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Publication Date
Fri Jan 15 2021
Journal Name
Plant Archives
ISOLATION AND IDENTIFICATION OF GBS BACTERIA FROM MASTITIS BY CAMP TEST AND LANCEFIELD’S SEROLOGICAL GROUPING
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Mastitis is an udder tissue inflammation which has infected various species of animals. It happens through several types of pathogenic bacteria, particularly Streptococcus agalactiae. GBS is a leading cause of cow mastitis. In our sample, 9.52% of Streptococcus agalactiae were isolated which were collected from bovine mastic milk and identified by biochemical tests such as catalase, oxidase, Production of indole, fermentation of sugar, an examination of antibiotic sensitivity, CAMP test and group kits of Lancefield. The results showed that all Streptococcus agalactiae isolate was diagnosed by CAMP test by the appearance of the arrowhead in blood agar and by the appearance of visible agglutination on a card in the serological grouping kit of

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Crossref
Publication Date
Sun Jul 29 2018
Journal Name
Iraqi Journal Of Science
Isolation and identification of Citrobacter freundii from chicken meat samples using cultural and molecular techniques
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Because of Citrobacter freundii medical and economical importance and that there are only little local studies about it, this study aimed to isolate and identify this important bacterial species from others that have a similar biochemical and morphological characteristics. Twenty five chicken meat samples were collected randomly from local markets in Baghdad city during 2017; Citrobacter was isolated from the collected samples using selective and differential media and identified using biochemical tests, the identification was confirmed using Vitek 2 compact and polymerase chain reaction for 16S rRNA and the isolated bacteria identified as C. freundii.

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Publication Date
Sun Jan 19 2020
Journal Name
Biochemical And Cellular Archives
Isolation and characterization of phosphate solubilizing Pseudomonas species and assess its efficacy as plant growth promoter
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Publication Date
Wed Dec 30 2020
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Identification and Isolation of Caffeic, Chlorogenic and Ferulic Acids in Aerial Parts of Capparis spinosa wildly grown in Iraq
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Capparis spinosa is one of the oldest genera grown in Iraqi land with worldwide traditional medicinal uses beside the culinary uses. These uses were own to the presence of many phytochemical including flavonoids, polyphenols. Among the reported polyphenolic acids are caffeic, chlorogenic and ferulic acids with well-known powerful antioxidant properties. The present work aimed to identify the presence of these polyphenolic acids in Iraqi caper naturally gown in the rural area of middle Iraq following standard chromatographic procedures. Aerial parts of the plant (buds, berries and leaves) were extracted with hydroalcoholic solvent by maceration method. Thin layer chromatographic techniques and HPLC analysis were performed to iden

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Publication Date
Sun Jul 02 2023
Journal Name
Iraqi Journal Of Science
Isolation and Identification of Vancomycin-Resistant Enterococcus Faecalis
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One hundred thirty - five clinical specimens of urine, blood, teeth root canal and burns were obtained from patients in hospitals of Baghdad. The specimens were cultured on Pfizer Selective Enterococcus agar to purify Enterococci isolates. 20 E. faecalis isolates were identified biochemically by growing in 10Cº, 45Cº, 6.5% NaCl, at pH 9.6 and confirmed by VITEK. Determination of Vancomycin-Resistant E. faecalis isolates were done by the minimum inhibitory concentrations [MICs] using agar dilution method. Seventeen E. faecalis isolates were determined as Vancomycin-Resistant and Intermediate Resistant.

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Isolation and Identification of Vancomycin-resistant Staphylococcus aureus
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Susceptibility of thirty seven clinical isolates of Staphylococcus aureus to various antibiotics was tested. 100 % of tested isolates were resistant to ampicillin, while the lowest resistance recorded to amikacin 8.10 %. Four of S. aureus isolates showed resistant to vancomycin. Minimum inhibitory concentration (MIC) of isolates 33 and 56 for vancomycin was ≥ 32 μg/ml.

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