Denture cleansing is an essential step that can stop cross‑contamination and adds to the health of the patient, denture durability, and the general quality of life. A disinfection technique must be practical and devoid of damaging effects on the material's properties used to construct the denture base. The main aim of this study is to evaluate the effect of three concentrations of electrolyzed water denture cleanser on heat cure acrylic and polyamide after immersion in electrolyzed water. The evaluation is based on their efficacy on surface hardness, wettability, and color stability compared with one submerged in distilled water as a control group. The method consists of eighty samples of heat-cured acrylic and polyamide material. The samples were immersed in electrolyzed water at a concentration of (100-200ppm) and in distilled water for 5 minutes, 30 times, and daily for 12 days to simulate a one-year interval. The tests showed that the surface hardness and color stability were maintained, with no significant difference between the control and experimental groups. In comparison, the result of wettability showed a statistically significant difference between the control and experimental groups. Thus, electrolyzed water does not affect the surface hardness and color stability of heat-cure acrylic and polyamide denture base materials. However, the wettability of these materials was significantly increased. Keywords: Electrolyzed water; Heat cure acrylic; Polyamide material