In present study 74 specimens of urine were collected from patients suffering from urinary tract infections.Fifty (67.56%) isolates were identified as Escherichia coli. 78% of isolates were identified as extendedspectrum beta lactamases (ESBL) producer. Antibiotic susceptibility t est was done and ceftazidime wasselected to complete this study by implying stress at sub-MIC on isolate harbor high number of resistancegenes (N11) and compared with sensitive isolate (S). Only four β-lactamase coding genes were detected;blaTEM, blaPER, blaVIM and blaCTX-M-2 and N11 had blaTEM, blaPER, and blaVIM. It was found that the resistantisolate did not form biofilm when compared with the sensitive one, which formed moderate biofilm. Inaddition, ceftazidime stress reduced the ability to produce slime layer and affected the viable bacterial countin combination with pH and temperature stresses. (PDF) Effectiveness of some β-lactamase encoding genes on biofilm formation and slime layer production by uropathogenic Escherichia coli. Available from: https://www.researchgate.net/publication/326088490_Effectiveness_of_some_b-lactamase_encoding_genes_on_biofilm_formation_and_slime_layer_production_by_uropathogenic_Escherichia_coli [accessed Jan 18 2025].
Apple vinegar has many uses that include burn and wound healing and as an antimicrobial agent against different microorganisms, but not as a solvent. Therefore, this study aimed to use commercial apple vinegar as solvent to the plants of roselle (Hibiscus sabdariffa), green tea (Camellia sinensis), and clove (Syzygium aromaticum). The effects of apple-vinegar extracts of these plants were compared with those of aqueous and ethanolic extracts against biofilm formation by Candida genus. Clove vinegar extract demonstrated antibiofilm activity against C. albicans, alone (2.4907± 0.382) or in combination with the antifungal agents fluconazole (1.689±0.33), nystati
... Show MoreFive isolates (25%) of Klebsiella pneumoniae were isolated from urine samples. In addition also isolated bacteria were (10) 50% Escherichia coli, while (3)15% Proteus spp., (2)10% Pseudomonas aeruginosa. The ethanolic extract of Cinnamomum zeylanicum bark were tested against Klebsiella pneumoniae by using the well agar diffusion test, the alcoholic bark extract from (200 -12.5) mg/ml possessed antimicrobial activity against tested microorganism. At 200 mg/ml, and 100 mg/ml concentrations was diameter of inhibition zone rang from (18-26mm), (14-16mm) respectively, and these results compared to antibiotics Norfloxacin(10µg) inhibition zone (24-30mm), and Cefotaxim (10 µg) (26-27mm) as
... Show MoreThe objective of this study was to investigate the prophylactic roles of human enteric derived Lactobacillus plantarum L1 (Ll) and Lactobacillus paracasei L2 (L2), on EHEC O157:H7 infection in rodent models (In vivo). The Lactobacillus suspensions (L1 and L2) were individually and orally administered to experimental rats at a daily two consecutives of 100 μl (108 CFU/ ml/rat) for up to two weeks. Thereafter, on the 8th day of experiment rats were orally challenged with one dose infection of EHEC (105 CFU/ml/rat). Animals mortality and illness symptoms have been monitored. There was no fatal EHEC infection in rats that had been pre‑colonized with the Lactobacillus strains, while most of EHEC infected rats were died (90%). The
... Show MoreOne of the costliest problems facing the production of hydrocarbons in unconsolidated sandstone reservoirs is the production of sand once hydrocarbon production starts. The sanding start prediction model is very important to decide on sand control in the future, including whether or when sand control should be used. This research developed an easy-to-use Computer program to determine the beginning of sanding sites in the driven area. The model is based on estimating the critical pressure drop that occurs when sand is onset to produced. The outcomes have been drawn as a function of the free sand production with the critical flow rates for reservoir pressure decline. The results show that the pressure drawdown required to
... Show MoreProteases have various applications in the food, pharmaceutical, medicine, pathogenicity of some pathogenic bacteria, and detergent sectors as well as meeting the needs of approximately 60% of the global enzyme industry, whereas they catalyze the breakdown of protein molecules into peptides and amino acids. Production and purification of protease enzyme by the isolate Escherichia coli AJ55 was scrutinized in the present study. Cultivation optimum conditions, were various complex medium, carbon source, nitrogen source, temperature, pH of the medium, and time of incubation were optimized to enhance the total protease production in shake flask culture of E.coli AJ55. The nutrient broth supplemented with 2% gluco
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