Preferred Language
Articles
/
TRglXJQBVTCNdQwCIBNb
SYNERGISTIC EFFECTS OF NEEM OIL AND GENTAMICIN ON PSEUDOMONAS AERUGINOSA VIA PHZM GENE DOWNREGULATION: A COMPREHENSIVE REVIEW
...Show More Authors

Exploring the antibacterial potential of neem oil (Azadirachta indica) in combination with gentamicin (GEN) against pathogenic molds, especially Pseudomonas aeruginosa, has drawn concern due to the quest for natural treatment options against incurable diseases. Prospective research directions include looking for natural cures for many of the currently incurable diseases available now. microbial identification system, were used to identify the isolates. The research utilized a range of methods, such as the diffusion agar well (AWD) assays, TEM (transmission electron microscopy) analysis, minimum inhibitory concentration (MIC) assays, and real-time PCR (RT-qPCR) to analyze bacterial expression and the antibacterial action of neem oil (Azadirachta indica) combined with gentamicin (GEN) against the pathogenic bacteria Pseudomonas aeruginosa between others. The combined effects of neem oil and GEN on P. aeruginosa are extensively investigated in this study, with particular attention paid to the downregulation of the virulent factor gene phzM, inhibition of biofilm formation, morphological changes, and gene expression, in that proportion. Results show that neem oil has strong antibacterial activity against P. aeruginosa and S. aureus, preventing the formation of biofilms and causing morphological alterations. In addition, the synergistic effects of neem oil and GEN are demonstrated, together in order with the downregulation of the phzM gene in P. aeruginosa. These results highlight the increased effectiveness of neem oil when combined with GEN and point to the potential of the oil as a stand-alone antibacterial agent. They also highlight the need for more study to overcome antibiotic resistance in harmful bacteria. Overall, our results point to a possibility that neem oil extract, either alone or in combination with GEN, can suppress the development of pathogenic microbes. This synergistic impact is related to the downregulation of a virulence factor gene. To address the drug resistance linked to pathogenic microbes, greater study is required. Novelty of study addresses the important issue of antibiotic resistance by exploring the potential synergistic effects of neem oil and gentamicin on pathogenic bacteria, particularly Pseudomonas aeruginosa. This topic is highly relevant in the context of the global challenge of antimicrobial resistance. Novelty and relevance the important issue of antibiotic resistance by exploring the potential synergistic effects of neem oil and gentamicin on pathogenic bacteria, particularly Pseudomonas aeruginosa. This topic is highly relevant in the context of the global challenge of antimicrobial resistance.

Clarivate Crossref
View Publication
Publication Date
Sun Jul 29 2018
Journal Name
Iraqi Journal Of Science
Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa
...Show More Authors

In the present study, the effect of vasicine alkaloid separated from Adhatoda vasica as an inhibitor agent on the activity of proteases enzyme isolated from Pseudomonas aeruginosa was investigated. forty isolates of Pseudomonas aeruginosa were collected from local hospital in Baghdad and then their ability for producing proteases was screened using quantification and semi- quantitative methods. Pseudomonas aeruginosa P1 was selected as the highest protease producer, which next identified as P. aeruginosa. It was found that the optimum culture conditions for protease production in submerged culture was in the tryptic - soya broth medium at 37° C with pH 8 for 48 hours. In addition, the study i

... Show More
View Publication Preview PDF
Publication Date
Wed Aug 30 2023
Journal Name
Iraqi Journal Of Science
Review of Smishing Detection Via Machine Learning
...Show More Authors

     Smishing is a cybercriminal attack targeting mobile Short Message Service (SMS) devices that contains a malicious link, phone number, or email. The attacker intends to use this message to steal the victim's sensitive information, such as passwords, bank account details, and credit cards. One method of combating smishing is to raise awareness and educate users about the various tactics used by SMS phishers. But even so, this method has been criticized for becoming inefficient because smishing tactics are continually evolving. A more promising anti-smishing method is to use machine learning. This paper introduces a number of machine learning algorithms that can be used for detecting smishing. Furthermore, the differences and simil

... Show More
View Publication Preview PDF
Scopus (3)
Crossref (1)
Scopus Crossref
Publication Date
Thu Apr 01 2021
Journal Name
Biochemical & Cellular Archives
Impacts of Starvation Stress on Biofilm Formation and expression of Virulence Genes in Mono-and Mixed-species cultures of Pseudomonas Aeruginosa and Staphylococcus aureus
...Show More Authors

Staphylococcus aureus and Pseudomonas aeruginosa are the major globally distributed pathogens, which causes chronic and recalcitrant infections due to their capacity to produce biofilms in large part. Biofilm production represents a survival strategy in these species, allowing them to endure environmental stress by altering their gene expression to match their own survival needs. In this study, we co-cultured different clinical isolates of S. aureus and P. aeruginosa as mono- and mixed-species biofilms in a full-strength Brain Heart Infusion Broth (BHI) and in a 1000-fold diluted Brain Heart Infusion Broth (BHI/1000) using Microtiter plate assay and determination of colony-forming units. Furthermore, the effect of starvation stress on the e

... Show More
Scopus (2)
Scopus
Publication Date
Thu Oct 01 2020
Journal Name
Biochemical & Cellular Archives
STUDY THE EFFECT OF LINUM USITATISSIMUM (FLAX SEEDS) AND EUCALPTUS ROSTRATA (ECALYPTUS) LEAVES EXTRACTS ON PSEUDOMONAS AERUGINOSA GROWTH IN VIVO AND IN VITRO
...Show More Authors

This study included the isolation and diagnosis of Pseudomonas aeruginosa from cases of burns samples. 100 samples were collected from resident patients at Al-Kindi Teaching Hospital, who suffer from different types of burns. Pseudomonas aeruginosa was isolated and diagnosed with 39% of the total samples. The cold and hot aqueous and alcoholic extract of flaxseeds oil was prepared and its inhibitory efficacy has been studied on the growth of isolated Pseudomonas aeruginosa. It is revealed that both extracts of flaxseeds oil had a high inhibitory effect on Pseudomonas aeruginosa growth. The Minimum Inhibitory Concentration (MIC) of flaxseed oil on the studied bacteria was also estimated, which was 25 mg/ml. In conclusion, the efficacy of fla

... Show More
Scopus
Publication Date
Thu Nov 01 2018
Journal Name
Journal Of Biological Sciences
Investigation of Plasmid-Associated Fluoroquinolone Resistance in Nosocomial Pseudomonas aeruginosa Isolated from Infected Burn Wounds
...Show More Authors

View Publication
Scopus (5)
Crossref (1)
Scopus Crossref
Publication Date
Fri Jun 01 2012
Journal Name
Journal Of Biotechnology Research Center
Determination the titer antibodies against LPS extracted from Pseudomonas aeruginosa isolated from eye infection
...Show More Authors

Preview PDF
Publication Date
Sat Oct 30 2021
Journal Name
Iraqi Journal Of Science
Occurrence of Point Mutations in gyrA and parC Genes of Ciprofloxacin-Resistant Pseudomonas aeruginosa Isolated from Burn Infections
...Show More Authors

     The spread of antibiotic resistant bacteria is a worldwide problem. Due to the importance of P. aeruginosa as a multidrug resistant bacterium, this study aimed, through molecular techniques, to detect point mutations in chromosomal genes responsible for the quinolones class of antibiotics resistance. A total of 52 isolates from burn infections were identified using specific primers for P. aeruginosa 16S rDNA. Ciprofloxacin minimum inhibitory concentrations (MIC) were estimated using the agar dilution assay. DNA sequences of the quinolone resistance-determining regions of gyrA and parC were determined for detecting the mutations found in these genes and the relations among the i

... Show More
View Publication Preview PDF
Scopus (1)
Crossref (1)
Scopus Crossref
Publication Date
Fri May 16 2014
Journal Name
International Journal Of Computer Applications
A Comprehensive Study of Smart Grids
...Show More Authors

In this paper, various aspects of smart grids are described. These aspects include the components of smart grids, the detailed functions of the smart energy meters within the smart grids and their effects on increasing the awareness, the advantages and disadvantages of smart grids, and the requirements of utilizing smart grids. To put some light on the difference between smart grids and traditional utility grids, some aspects of the traditional utility grids are covered in this paper as well.

View Publication Preview PDF
Crossref
Publication Date
Mon Feb 01 2021
Journal Name
Meta Gene
Association analysis of FTO gene polymorphisms rs9939609 and obesity risk among the adults: A systematic review and meta-analysis” Meta Gene (2020) 7–7/100832
...Show More Authors

Background: Obesity typically results from a variety of causes and factors which contribute, genetics included, and style of living choices, and described as excessive body fat accumulation of body fat lead to excessive body, is a chronic disorder that combines pathogenic environmental and genetic factors. So, the current study objective was to investigate the of the FTO gene rs9939609 polymorphism and the obesity risk. Explaining the relationship between fat mass and obesity-associated gene (FTO) rs9939609 polymorphism and obesity in adults. Methods: Identify research exploring the association between the obesity risk and the variation polymorphisms of FTO gene rs9939609. We combined the modified odds ratios (OR) as total groups and subgro

... Show More
View Publication
Publication Date
Wed Nov 01 2017
Journal Name
2017 7th World Engineering Education Forum (weef)
Sustaining a synergistic industrial advisory board and academic collaboration
...Show More Authors

View Publication
Scopus (1)
Crossref (1)
Scopus Crossref