Bromelain enzyme
Chemomechanical caries removal
Raman spectroscopy
Vickers microhardness
SEM
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To assess the biochemical, mechanical and structural characteristics of retained dentin after applying
three novel bromelain‑contained chemomechanical caries removal (CMCR) formulations in comparison
to the conventional excavation methods (hand and rotary) and a commercial papain‑contained gel
(Brix 3000). Seventy‑two extracted permanent molars with natural occlusal carious lesions (score > 4
following the International Caries Detection and Assessment System (ICDAS‑II)) were randomly
allocated into six groups (n = 12) according to the excavation methods: hand excavation, rotary
excavation, Brix 3000, bromelain‑contained gel (F1), bromelain‑chloramine‑T (F2), and bromelain
chlorhexidine gel (F3). The superficial and deepe
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