Preferred Language
Articles
/
TBaQqogBVTCNdQwC-now
Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry
Abstract<p>Construction of artificial higher order protein complexes allows sampling of structural architectures and functional features not accessible by classical monomeric proteins. Here, we combine in silico modelling with expanded genetic code facilitated strain promoted azide-alkyne cycloaddition to construct artificial complexes that are structurally integrated protein dimers and demonstrate functional synergy. Using fluorescent proteins sfGFP and Venus as models, homodimers and heterodimers are constructed that switched ON once assembled and display enhanced spectral properties. Symmetrical crosslinks are found to be important for functional enhancement. The determined molecular structure of one artificial dimer shows that a new long-range polar network comprised mostly of organised water molecules links the two chromophores leading to activation and functional enhancement. Single molecule analysis reveals the dimer is more resistant to photobleaching spending longer times in the ON state. Thus, genetically encoded bioorthogonal chemistry can be used to generate truly integrated artificial protein complexes that enhance function.</p>
Scopus Clarivate Crossref
View Publication
Publication Date
Thu Jun 01 2017
Journal Name
Journal Of The College Of Languages (jcl)
Formal and Functional Perspectives in the Analysis of Hyperboles in Obama's Speeches

     Hyperbole is an obvious and intentional exaggeration in the sense that it takes things to such an extreme that the audience goes too far and then pulls itself back to a more reasonable position, i.e. it is an extravagant statement or figure of speech not intended to be taken literally. This paper focuses on the formal and functional perspectives in the analysis of hyperbole which American candidates produce in their speeches in electoral campaigns, for it is hypothesized that candidates in their electoral campaigns use hyperbolic expressions excessively to persuade voters of the objectives of their electoral campaign programs. Hence, it aims to analyze hyperbole in context to determine the range of pragmatic func

... Show More
View Publication Preview PDF
Publication Date
Thu Dec 20 2018
Journal Name
مجلة العلوم الاجتماعية والتربوية (ريس)
The acquisition of the concepts of nanotechnology by the students of chemistry in the college of Education for pure Sciences/ibn Al-Haytham and it`s relation to their technological

One of the scientific education aims, preparing a student able to keep up with the scientific developments and innovations around him and make him contribute, adapt, investment and continue development. The concepts of nanotechnology from scientific innovation open up an important area of thinking and intervention in the field of chemistry applications in daily life, the technological changes lead to social, political and economic changes which result that the students to have the knowledge, understanding, awareness, appreciation and sense in applications of modern technology for their use optimally, in order to cope with these scientific and technological changes. The research aims at finding out the correlation between the acquisi

... Show More
Publication Date
Sun Jun 11 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Artificial Neural Network for TIFF Image Compression

The main aim of image compression is to reduce the its size to be able for transforming and storage, therefore many methods appeared to compress the image, one of these methods is "Multilayer Perceptron ". Multilayer Perceptron (MLP) method which is artificial neural network based on the Back-Propagation algorithm for compressing the image. In case this algorithm depends upon the number of neurons in the hidden layer only the above mentioned will not be quite enough to reach the desired results, then we have to take into consideration the standards which the compression process depend on to get the best results. We have trained a group of TIFF images with the size of (256*256)  in our research, compressed them by using MLP for each

... Show More
Crossref
View Publication Preview PDF
Publication Date
Sun Apr 29 2018
Journal Name
Iraqi Journal Of Science
Modified Artificial immune system as Feature Selection

Feature selection algorithms play a big role in machine learning applications. There are several feature selection strategies based on metaheuristic algorithms. In this paper a feature selection strategy based on Modified Artificial Immune System (MAIS) has been proposed. The proposed algorithm exploits the advantages of Artificial Immune System AIS to increase the performance and randomization of features. The experimental results based on NSL-KDD dataset, have showed increasing in performance of accuracy compared with other feature selection algorithms (best first search, correlation and information gain).

View Publication Preview PDF
Publication Date
Sat Jan 01 2011
Journal Name
Journal Of Engineering
FILTRATION MODELING USING ARTIFICIAL NEURAL NETWORK (ANN)

In this research Artificial Neural Network (ANN) technique was applied to study the filtration process in water treatment. Eight models have been developed and tested using data from a pilot filtration plant, working under different process design criteria; influent turbidity, bed depth, grain size, filtration rate and running time (length of the filtration run), recording effluent turbidity and head losses. The ANN models were constructed for the prediction of different performance criteria in the filtration process: effluent turbidity, head losses and running time. The results indicate that it is quite possible to use artificial neural networks in predicting effluent turbidity, head losses and running time in the filtration process, wi

... Show More
Crossref
View Publication Preview PDF
Publication Date
Sun Aug 01 2021
Journal Name
Journal Of Education And Science
Evaluation of Salivary Alpha-Amylase level in Iraqi children with positive family history of hypertension

This study accomplished to determine levels of salivary Alpha-Amylase (sAA) in Iraqi children with positive family history of hypertension, Hypertension was believed to be a risk factor in 18% of all deaths in 2010 (9.4 million globally). This study included one hundred children from primary school aged 6 to 13 years, with a focus in child welfare and family health history, with an emphasis on close relatives' hypertension reputations, with hypertension close relatives record categories identified by 1st and 2nd relatives, after documenting the full details, the burden was assessed for the children's families. The influence of age is confirmed by a correlation study of our own numbers, which shows a positive correlation. The disparity betwe

... Show More
Crossref
View Publication
Publication Date
Thu Oct 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Prevalence of BRCA1 Oncogen Expression in Breast Cancer Specimens of Patients with Positive Family History

Background: Breast cancer is the leading female cancer worldwide and in Iraq .Some mutations, particularly in BRCA1, significantly increase the risk of the disease.
Objectives: To demonstrate the frequency of BRCA1 in a group of high risk women with “positive family history’’ of breast cancer; correlating the immune expression of BRCA1 with some parameters of known prognostic significance.
Patients and Methods: Eighty-two female patients diagnosed with breast cancer (50 familial and 32 non familial) were included in the study .The mean age of the patients was 48.07. Immunohistochemistry was performed to assess the BRCA1 oncogene expression, Estrogen Receptor (ER), Progesterone Receptor (PR), Her 2 neu contents of the tumors.<

... Show More
Crossref
View Publication Preview PDF
Publication Date
Sat Oct 30 2021
Journal Name
Iraqi Journal Of Science
Detection of Genetic Polymorphism of HER2 Gene in HER2 Positive Breast Cancer Women in Iraq

     The human epidermal growth factor receptor-2 (HER2) gene plays a critical role in breast cancer development and progression. HER2 overexpression characterizes a biologically and clinically aggressive breast cancer subtype. In this study, 60 samples from Iraqi women with breast cancer were collected and investigated for HER2 protein in the tissue by immunohistochemistry. Also, 20 samples from healthy Iraqi women were used as a control. The results showed that 18 (30 %) patients expressed the HER2 protein. A molecular study for single nucleotide polymorphism (SNP) was conducted on samples metastasizing to lymph nodes. DNA was extracted and polymerase chain reaction (PCR) was performed to amplify e

... Show More
Scopus (1)
Crossref (1)
Scopus Crossref
View Publication Preview PDF
Publication Date
Wed May 08 2024
Journal Name
Asia Pacific Journal Of Molecular Biology And Biotechnology
Expression of Heat Shock Protein 70 in thyroid gland tumors

Heat shock protein 70 (HSP70) is a crucial protein with vital biological tasks in cell continuation of life. The variation of HSP70 activation occurs as a consequence of stress that includes temperature states, toxicity, poisoning with heavy metals, and tumor-related conditions. One of the master jobs of the HSP family is the suppression of caspase-mediated apoptosis signals. A high level of the expression of HSP70 is accountable for tumorigenesis and resistance against chemotherapeutic drugs. For this reason, the detection of HSP70 may help to diagnose cancerous diseases. From the other side, targeting this chaperone might help in treatment by maintaining late caspase-dependent events. This study was conducted to detect the presenc

... Show More
Scopus Crossref
View Publication Preview PDF
Publication Date
Fri Jan 01 2016
Journal Name
Animal Nutrition And Feed Technology
Scopus (18)
Crossref (13)
Scopus Clarivate Crossref
View Publication