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Gingival tissue samples from periodontitis patients demonstrate epithelial–mesenchymal transition phenotype
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Abstract<sec><title>Objective

To determine the expression of key epithelial–mesenchymal transition (EMT) markers in gingival tissue samples collected from patients with periodontitis.

Background

Epithelial–mesenchymal transition is a process responsible for shifting epithelial‐phenotype to mesenchymal‐phenotype leading to loss of epithelial‐barrier function. Thus, EMT could be involved as a pathogenic mechanism in periodontitis as both conditions share common promoters and signalling pathways.

Materials and Methods

Gingival tissue samples were collected from patients with periodontitis (case) and healthy periodontium (control). Periodontal parameters including bleeding on probing, probing pocket depth (PPD), and clinical attachment loss were recorded. Paraffinized tissue samples were processed and immunohistochemically stained to determine the expression of key EMT markers which included E‐cadherin, β‐catenin, Snail1 and vimentin.

Results

The majority of cases (n = 65, 72.2%) were diagnosed with periodontitis stage 3 or 4, grade b or c vs 25 (27.8%) subjects with intact healthy periodontium. Discontinuity of epithelium was detected in up to 80.9% of periodontitis cases associated with reduced number of epithelial layers as compared to controls. Immunohistochemical expression of epithelial markers (E‐cadherin and β‐catenin) was significantly downregulated in periodontitis patients as compared with controls. Periodontitis cases exhibited significant upregulation of Snail1 expression. Furthermore, cytoplasmic vimentin (66.2%) and nuclear β‐catenin (27.7%) were solely expressed in periodontally diseased tissues compared with control. Epithelial markers, E‐cadherin and β‐catenin, were significantly negatively correlated with increasing PPD, while vimentin showed positive correlation with this parameter.

Conclusion

There were marked downregulation of epithelial molecules and upregulation of mesenchymal markers in gingival tissues derived from periodontitis patients, suggesting expression of the EMT phenotype in the pathological epithelial lining of periodontal pockets.

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Thu Jan 30 2014
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THE PREVALENCE AND ANTIMICROBIAL RESISTANCE OF PSEUDOMONAS SPECIES IN PATIENTS WITH CHRONIC SUPPURATIVE OTITIS MEDIA
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Publication Date
Mon Apr 01 2019
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Possible beneficial effects of amlodipine, lisinopril, and their Combination on lipid profile in hypertensive patients
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Thu Jun 30 2011
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Prevalence of clinically significant Hepatopulmonary Syndrome among Patients with Chronic Liver Disease and Portal Hypertension
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Thu Jan 30 2014
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Publication Date
Sun Jul 01 2018
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Molecular and Serologic Detection of HLA-B27 among Ankylosing Spondylitis Patients with Some Clinical Correlations
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Publication Date
Fri Nov 09 2018
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Gene Expression and Methylation Levels of PCSK9 Gene in Iraqi Patients with Coronary Artery Disease
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Publication Date
Tue Jan 01 2019
Journal Name
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Evaluation of Oxidative Stress and Some Vitamins as Antioxidants in Type2 Diabetic and Nephropathic Patients.
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