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Introduction: The stringent response is a bacterial adaptation mechanism
triggered by stress conditions, including nutrient limitation. This
response helps bacteria survive under harsh conditions, such as those
encountered during infection. A key feature of the stringent response
is the synthesis of the alarmone (p)ppGpp, which influences various
bacterial phenotypes. In several bacterial species, stringent response
activation significantly affects biofilm formation and maintenance.
Methods: Clinical specimens were collected from multiple hospitals in
Baghdad, Iraq. Staphylococcus aureus was identified using conventional
biochemical tests. The PCR technique was applied to detect mecA,
icaA, and icaD genes, while the Vitek 2 compac
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