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Resistance Efficiency of Some Bacterial Isolates Against Malathion Pesticide
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Chemical pesticides have an impact on other living organisms in addition to their intended target organisms. Any chemical pesticide is therefore made safe for use by examining its biological characteristics and side effects. The present study was aimed at determining the resistance efficiency of six bacterial isolates obtained from malathion-contaminated soils. Bacteria were isolated from soil samples collected in Adhamiya, Baghdad, Iraq. Biochemical tests and VITEK 2 compact equipment were used to identify the bacterial isolates. Primary and secondary screening tests were conducted on the bacterial isolates for resistance against malathion pesticides. The optimal bacterial growth conditions were determined in malathion-contaminated media. The results demonstrated that the bacterial isolates 1, 3, 4, and 8 grew best on malathion-contaminated (100 mgL-1) mineral salt medium (MSM). Isolates 1 and 2 had a MIC of 500 mgL-1, where they continued to grow until the seventh day of incubation. Pseudomonas aeruginosa, Pseudomonas putida, Aeromonas hydrophilia, and Escherichia coli were the identified bacterial isolates. These isolates showed optimal bacterial growth in the test conditions (temperature, incubation period, and pH), indicating their efficiency and ability to degrade malathion. The highest average growth of P. aeruginosa at 35 °C was 70.08 mm, while that of P. putida was 40.10 mm during the 7-day incubation period. Also, the highest values of average growth were observed in the same bacteria at pH 7, with a value of 26.98 mm. The findings of this study reveal that Pseudomonas aeruginosa and Pseudomonas putida were the best bacterial isolates for biodegrading malathion.

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Publication Date
Wed Jan 01 2020
Journal Name
University Of Bucharest Rom Biotechnol Printed In Romania.
Antibiotic resistance determinants of Acinetobacter baumannii strains isolated from nosocomial infections
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Publication Date
Sat Sep 30 2023
Journal Name
Iraqi Journal Of Science
Study on tssC1 Gene Mediating Biofilm Antibiotics Resistance of Pseudomonas aeruginosa
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P. aeruginosa is a famous bacterium that causes several diseases and has a high ability to be a multidrug resistant organism that is linked with the formation of biofilm. This study aimed to investigate tssC1 gene role in the resistance of different antibiotics in the presence of biofilm. We constructed biofilm for the isolates under the study and showed the effect of different antibiotics on biofilm formation and maturation. The presence of the gene was detected through achieving PCR reaction. Finally, tssC1 gene variation was determined through sequencing and aligning the sequencing products. The results showed that most of the isolates (80%) formed biofilm that played a role in the resistance of different antibiotics which could be du

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Publication Date
Sat Jul 22 2023
Journal Name
Journal Of Engineering
Investigation Parameters of Resistance Spot Welding For AA1050 Aluminum Alloy Sheets
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The parameters of resistance spot welding (RSW) performed on low strength commercial aluminum sheets are investigated experimentally, the performance requirements and weldability issues were driven the choice of a specific aluminum alloy that was AA1050. RSW aluminum alloys has a major problem of inconsistent quality from weld to weld comparing with welding steel
alloys sheet, due to the higher thermal conductivity, higher thermal expansion, narrow plastic temperature range, and lower electrical resistivity. Much effort has been devoted to the study of describing the relation between the parameters of the process (welding current, welding time, and electrode force) and weld strength. Shear-tensile strength tests were performed to ind

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Publication Date
Wed Apr 26 2023
Journal Name
Journal Of Contemporary Medical Sciences
A potential role of extracellular DNA in biofilm and ciprofloxacin resistance
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Objectives: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates. Methods: Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in gyrA and parC. Results: The biofilm intensity significantly decreased (P < 0.05) concerning S. aureus isolates and insignificantly (P > 0.05) concernin

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Publication Date
Sat Sep 30 2023
Journal Name
Iraqi Journal Of Science
Study on tssC1 Gene Mediating Biofilm Antibiotics Resistance of Pseudomonas aeruginosa
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P. aeruginosa is a famous bacterium that causes several diseases and has a high ability to be a multidrug resistant organism that is linked with the formation of biofilm. This study aimed to investigate tssC1 gene role in the resistance of different antibiotics in the presence of biofilm. We constructed biofilm for the isolates under the study and showed the effect of different antibiotics on biofilm formation and maturation. The presence of the gene was detected through achieving PCR reaction. Finally, tssC1 gene variation was determined through sequencing and aligning the sequencing products. The results showed that most of the isolates (80%) formed biofilm that played a role in the resistance of different antibiotics which could

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Publication Date
Sun Oct 27 2019
Journal Name
Iraqi Journal Of Science
The Effect of Alcoholic Extracts of Zingiberofficinale Anti-E.Coli Isolates Isolated from Urinary Tract Infection
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The Escherichia coli isolated from UTIs, were identified with biochemical tests and vitek test.The ethanolic extracts of Zingiberofficinale(Z.officinale) were tested against E.coli by using the good agar diffusion test, the alcoholic extracts from (25 -100) mg/ml showed antimicrobial activity against tested microorganism. The diameter of inhibition zone increase at high concentrations and rang from (18-20mm) , and these results compared with antibiotics  sensitivity discs were used by discs diffusion method against E.coliisolates, they were resisted to all antibiotics used in this study. It could be concluded that alcoholic extract of Z.officinalehad good antimicrobial effects, and may be able to use for treatment UTIs caused by E.c

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Publication Date
Tue Jan 30 2024
Journal Name
Iraqi Journal Of Science
Detecting the Variation in the lasI Gene and Their Relation with Biofilm in Pseudomonas aeruginosa Isolates
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     One of the most causative agents for many opportunistic diseases is the Pseudomonas aeruginosa which has a high percentage of multidrug resistance disease through construction of biofilm. The current study aimed for evaluating the correlation between quorum sensing genes (which is lasI gene) and biofilm formation. The biofilm construction and antibiotics susceptibility test were achieved for all the isolates under the study. The PCR and sequencing techniques were also carried out to detect the type of variation in lasI gene for each scheme of biofilm formation (weak, strong, and moderate). High antibiotic resistance was recorded among biofilm producing isolates. The genic pattern for the weak biof

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Publication Date
Wed Apr 17 2019
Journal Name
Iraqi Journal Of Science
Effect of Fiber (Glass, Poly Propylene) on Hardness, Water Absorption And Anti-Bacterial Activity of Coating Acrylic Polymer
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Coating materials have an extraordinarily high use, for industrial or technical applications. This study compares the effect of short glass and polypropylene fibers on the hardness, water absorption and anti-bacterial activity of acrylic composites against Gram positive (Streptococcus sp.) and Gram negative bacteria (E.coli, Pseudomonas aeruginosa, Klebsiella sp.).To prepare acrylic polymer and acryliccomposites (glass fibers and polypropylene fiber) the weight fraction (20 %,) (v0= lama acrylic, v1= lama acrylic/ glass fiber, and v2= lama acrylic /poly propylene fiber), of chopped glass fibers and polypropylene were added to acrylic , the resultant solution was stirred by hand for 5 minutes, using the Hand-lay-up technique.
Results s

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Publication Date
Sun Jun 11 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Evaluation of Inhibitory Efficiency for Some Aqueous and Alcoholic Extracts of Cumin, Fenugreek, Sweet Fennel and Black Cumin in Growth of Escherichia Coli and Staphylococcus Aureus Bacteria
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  A factorial experiment was conducted at the laboratories of the College of Agriculture – Kerbala University during 2016. The aim was inhibitory efficiency for some aqueous and alcoholic extracts of Cumin, Fenugreek, Sweet Fennel and Black cumin in growth of Escherichia coli and Staphylococcus aureus bacteria. Results of Lab the extracts alcoholic, Concentrations 10, and 20 μg/ml giving to the highest percentage of inhibition from water extracts for both types of bacteria. Alcoholic extract of cumin highest percentage inhibition and concentration reached 23 and 26 mm, respectively, for the bacterium Staphylococcus aureus, while the bacteria Escherichia coli giving the alcoholic extract of the concentration of 20 μg/ml

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Publication Date
Tue Sep 01 2020
Journal Name
Iraqi Journal Of Physics
Enhancment of the corrosion resistance of copper metal by laser surface treatment
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In this work, the copper metal was treated using Nd:YAG laser with energy 1Joul to enhance corrosion resistance and improve surface properties. The copper metal has many applications in industry as well as water, oil and gas pipes. The same conditions, (laser power density, scan speed, distance between paths, medium gas-air) were applied in the laser surface treatment, After laser treatment, the samples microstructures were investigated using optical microscope (OM) to examine micro structural changes due to laser irradiation. Specimen surfaces were investigated using atomic force microscopy (AFM), X-ray diffraction (XRD), macro hardness, and corrosion test before and after laser treatment to

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