Semliki Forest Virus (SFV), a member of the Alphavirus genus in the Togaviridae family, is a small-enveloped, positive-sense single-stranded RNA (+ssRNA) virus. The virus is spread by mosquitos and can infect humans, resulting in mild febrile disease with symptoms that include fever, myalgia, arthralgia, persistent headaches and asthenia. Virulent strains of SFV in mice cause lethal encephalitis by infecting neurons in the central nervous system. In on-going experiments in the research group using a focused siRNA screen we have investigated the role of deubiquitylases (DUBs) during SFV infection (as a model alphavirus) and monitored the effect of DUB depletion on cell viability after infection. We identified a group of DUBs that have a pro-viral effect. The DUB, USP5, from this screen was validated to determine its effect upon viral replication. Here, we show that depleted USP5 in HeLa cells resulted in SFV RNA and viral yield at 8 h post-infection being significantly reduced. In the multi-step viral growth curve assay, in the absence of USP5, similar yields of SFV were determined at 2 and 4 h post-infection. However, a significant reduction in the infectious viral particles release at 6, 8, 10 and 12 h post-infection was observed and this could be reversed by direct constraining viral replication. These results raise the potential for USP5 to play a distinct role in the replication of SFV, suggesting that USP5 may be a possible anti-viral therapy for alphavirus infection.
The question of motherhood seems to haunt the works of Kate Chopin, a
nineteenth century American writer. Though a mother-woman herself, Chopin
gives a paradoxical portrait of motherhood in her works. She implies that
motherhood might be a liberating experience for some women as it is constricting
for others. This paper tackles the paradoxical nature of motherhood in Chopin's
novel, The Awakening and other four short stories. In The Awakening, A Pair of
Silk Stockings and Desiree's Baby, motherhood is depicted as a tool of selfdestruction,
while in Athénaise and Regret, it is a source of life-giving.
The research sheds light on the subject of phraseology as an important field to present metaphysical issues with contemporary scientific discourse. (Primary psychology) It does not apply with the reality of this science and therefore some tried to call it other names such as (Alsai), a unit of measurement of energy activity of things and assets. It was also called metaphysics or metaphysics, but it eventually settled on parapsychology. We try to win Islam and religious concepts with contemporary scientific discourse. The researcher discussed issues that modern science was unable to comprehend and interpreted. He used these subjects to prove the truths of religion and faith. Modern times because the whole universe miracles or as Imam Ghaz
... Show MoreReview Article Immunomodulatory Role of Cytokines in Periodontal Disease Batool Hassan Al-Ghurabi*, Maha Adel Mahmood, Zainab A. Aldhaher, Sahar Hashim Al-Hindawi Adv. life sci., vol. 11,...
The current study aims to examine the level of problems faced by university students in distance learning, in addition to identify the differences in these problems in terms of the availability of internet services, gender, college, GPA, interactions, academic cohort, and family economic status. The study sample consisted of (3172) students (57.3% females). The researchers developed a questionnaire with (32) items to measure distance learning problems in four areas: Psychological (9 items), academic (10 items), technological (7 items), and study environment (6 items). The responses are scored on a (5) point Likert Scale ranging from 1 (strongly disagree) to 5 (strongly agree). Means, standard deviations, and Multivariate Analysis of Vari
... Show MoreWith wide spread of internet, and increase the price of information, steganography become very important to communication. Over many years used different types of digital cover to hide information as a cover channel, image from important digital cover used in steganography because widely use in internet without suspicious. Since image is frequently compressed for storing and transmission, so steganography must counter the variations caused by loss compression algorithm. This paper describes a robust blind image steganography, the proposed method embeds the secret message without altering the quality by spraying theme on the blocks in the high order bits in color channel s
... Show Morethis research adopts the study of experimentational fiction in the characters for the writer Fadhil Al-Azzawi ,which appear in the miraculous characters that the writer creates in his enumerative paragraphs , and these characters are featured by there reveling on traditions which is prevalent in the formation of these characters, The research also included theoretical beginning foe experimental fiction and miraculous characters, followed by analysis and examination of these characters in the writer`s texts, finishing by an epilogue for the results of the research , giving the references and resources we depended on writing this research .
Pancreatic adenocarcinoma is one of the major causes of cancer death in the world. Alterations in p53 tumor suppressor gene
Feminism is a diverse set of social theories, political movements and ethos driven by women's causes. Feminists and feminists agree that the ultimate goal is to eliminate the forms of gender-related oppression It should be noted that feminism originated at the beginning as an emancipation movement aimed only at lifting the injustice and suffering of European women, but over time evolved and crystallized to form an independent thought in itself swimming against all the universals known to humanity in the relationship between male and female nature This study posits a hypothesis that the feminist movement is characterized by multiple and varied trends, different in its analyzes of the causes of women's oppression, but all agreed on the need t
... Show MoreIn this paper, the concept of contraction mapping on a -metric space is extended with a consideration on local contraction. As a result, two fixed point theorems were proved for contraction on a closed ball in a complete -metric space.
The researcher wanted to make an attempt to identify the foundations of social solidarity, to strengthen the bonds of brotherhood among society, and spread the causes of compassion in the hearts of its members.
The researcher has taken a short course in the hearts of the beloved to hearts.