Pseudomonas aeruginosa
mexB efflux gene
Antibiotic resistance
Inhibition of efflux pump
Curcumin
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According to the prevalence of multidrug resistance bacteria, especially
Pseudomonas aeruginosa, in which the essential mechanism of drug resistance is the
ability to possess an efflux pump by which extrusion of antimicrobial agents usually
occurs, this study aims to detect the presence of mexB multidrug efflux gene in some
local isolates of this bacteria that show resistance towards three antibiotics, out of
five. Sensitivity test to antibiotics was performed on all isolates by using
meropenem (10μg/disc), imipenem (10μg/disc), amikacin (30 μg/disc),
ciprofloxacin (5μg/disc) and ceftazidime (30 μg/disc). Conventional PCR results
showed the presence of mexB gene (244bp) in four isolates out of ten (40%). In
addition,25, 50μg/ml of cur
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