The study was carried out in plant tissue culture laboratory, University of Baghdad during the period 2017-2019, as factorial experiment in complete randomized design, to study the effect of PEG at (0, 2, 4, 6 and 8%) on physiological and chemical changes in callus of three sunflower (Ishaqi 1, Aqmar and Al-haga) induced by the cultivation of the young stem in vitro under water stress. The content of callus cells of SOD, POD, CAT and APX enzymes as well as total dissolved carbohydrate were determined as indicators to determine the effect of PEG in callus tissue cells cultivated on medium equipped with the PEG concentrations. The results showed that cultivars were differs significantly, and A-haja variety was superior in increasing SOD to 12.26 absorption unit mg-1 protein, and the variety Ishaqi was superior in increasing POD, CAT and APX to 7.25, 11.54 and 0.4285 absorption unit mg-1 protein respectively. Aqmar variety was superior in increasing the total dissolved carbohydrate content to 0.34980 mg-1. PEG concentrations significantly affected enzyme activity, and 6% gave the highest efficacy of SOD and APX reached to 13.78 and 0.2976 absorption unit mg-1 protein respectively, while 8% PEG gave the highest effective of POD and CAT reached to 5.54 and 7.73 absorption unit mg-1 protein respectively. The total dissolved carbohydrate content achieved the highest mean at 8% PEG and reached to 0.31, 128 mg. g-1. The interaction caused significant effect and the highest SOD enzyme was obtained from Al-haja variety at 6% PEG, while POD, CAT, and APX enzymes (11.33, 16.30, and 0.5208 unit absorption mg-1 protein) were obtained …
The current study dealt with the effect of water extract of pomegranate peel plant on Oscillatoria amoena growth that isolated from Diwaniya river, The effect of the pomegranate extract was measured by calculating the total number of cells and the absorbance values of the alga. Three concentrations were used 3.5, 7 and 14 mg/ml from extract of pomegranate fruit peel in addition to the control group. The results showed that exposing the alga to concentrate 14 mg/ml led to lower growth sharply, while the rest of the concentrations 3.5 and 7 mg/ml also decreased the growth gradually. The absorbance values showed a decline similar to the number of cells during the period of the exposure.
Effect of drought stress induced by mannitol on secondary products in callus of Golden Sunrise cherry tomatoes were studied in vitro. Seeds were irradiated with gamma at the doses (0, 20, or 40 Gy), and germinated on Murashige and Skoog, (MS) basal medium. Callus were initiated from cotyledon leaf explants using a combination of (2.0 kin + 2.0 IAA mg. l-1) then transferred into MS medium supplemented with mannitol at concentrations of (0, 40, and 60 g.l-1). Vitamin C(vit C), enzyme activity, Ascorbate peroxidase (Apx), and Amylase enzymes, Proline amino acid and β- carotene were quantified by high-performance Performance Liquid Chromatography (HPLC). Results revealed that, highest contents of (Apx) 128.16 µg. m
... Show MoreBackground: The miswak is a teeth cleaning twig made from a twig of the Salvadora persica tree (known as arak in Arabic).A traditional alternative to the modern toothbrush, it has a long, well-documented history and is reputed for its medicinal benefits. It also features prominently in Islamic hygienical jurisprudence. Materials and methods: Twenty maxillary first premolars were treated with the selected solutions which included siwak water extract (5%, 10%) and sodium fluoride 0.05% for 2minutes once daily for 20 days interval, deionized water was used as control negative. Then the concentration of the dissolved calcium ion in the etching solution of 2N HCL was measured. Results: The least amount of the dissolved calcium ion was registere
... Show MoreAntimicrobial and antiyeast activity of ethanolic and aqueous extract of grape fruit seed (Citrus paradise ; Rutaceaa) was examined against 10 bacterial and 2 yeast strains. The level of the antimicrobial effects was established using an in vitro agar assay and minimum inhibitory concentration (MIC). In general ethanolic extract were more effective on gram positive bacteria than gram negative bacteria and strongest antimicrobial effect against Streptococcus pyogenes and Salmonella entritidis. Other tested bacteria and yeasts were sensitive to extract ranging from 4 to 16 mg/ml and more.
This study was aimed to investigate the role of crud alcoholic extract of Lallemantia royleana seeds in reducing the hepatotoxicity and side effect of rifadin drug in liver. The animals (40 mice) were divided into four groups, the first group was treated with normal saline (0.9%) for 28 days as a control and the second group was treated with rifadin (1.5 mg/kg/day) for 28 days and third group was treated with acoholic extract of Lallemantia royleana seeds (1% w/v) for 28 days, while the forth group was treated with alcoholic extract of seeds alone for 5 days and with alcoholic extract and rifadin for 28 days, so the total period of this group is 33
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In this manuscript, a simple new method for the green synthesis of platinum nanoparticles (Pt NPs) utilizing F. carica Fig extract as reducing agent for antimicrobial activities was reported. Simultaneously, the microstructural and morphological features of the synthesized Pt NPs were thoroughly investigated. In particular, the attained Pt NPs exhibited spherical shape with diameter range of 5-30 nm and root mean square of 9.48 nm using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), respectively. Additionally, the final product (Pt NPs) was screened as antifungal and antibacterial agent against Candida and Aspergillus species as well as Gram-positive Staphyllococcus aureus and G
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