Some Factors determining the virulence of Escherichia coli ( E. coli ) isolates were studied ,of 25 isolates , 17(group A) uropathogenic E. coli ,6 (group B) infected gastrointestinal tract , 2 (group C) infected wound , beside these group we use the standard strain E. coli HB101 as control group. The twenty five isolates were tested for adherence capability to human buccal cavity epithelial cells by in vitro experiment . The results showed that all isolates have different adhesion capability with mean ranging from (14.35±11.39) to (33.80 ± 22.68) bacteria / epithelial cell It was noticed that isolates EU9, ES6, EW17 displayed high adhesive capability with mean value (33.80 ± 22.68), (32.60 ± 21.19), (29.90±22.50) bacteria /epithelial cell respectively, while the isolates EU4 displayed a lowest adhesive capability with mean value of (14.35±11.39) bacteria / epithelial cell. It was also found that there was no significant difference ( P ≥ 0.001) for adhesive capability among EU9, ES6, EW17, but the adhesive capability for these isolates was significantly higher (P ≤ 0.001) compared to standard strain which showed adhesive capability with mean value (0.55± 0.88),thus these isolates were selected for further experiments to study others virulence factors such as ability to agglutination human , rabbit , sheep erythrocytes in mannose – sensitive manner and production of haemolysin , biofilm. The results revealed that all isolates had nearly the same hemagluttination pattern in the absence of D-mannose while the pattern is different in the presence of D- mannose, where the isolates EU9, ES6 show ability to agglutinate human and sheep erythrocytes and so considered as Mannose Resistant Hemagluttination (MRHA) while the isolate EW17 did not show this ability and considered as Mannose Sensitive Hemagluttination ( MSHA). It was also found that all isolates have ability to produce haemolysin and biofilm formation but in different pattern.
Nuclear shell model is adopted to calculate the electric quadrupole moments for some Calcium isotopes 20Ca (N = 21, 23, 25, and 27) in the fp shell. The wave function is generated using a two body effective interaction fpd6 and fp space model. The one body density matrix elements (OBDM) are calculated for these isotopes using the NuShellX@MSU code. The effect of the core-polarizations was taken through the theory microscopic by taking the set of the effective charges. The results for the quadrupole moments by using Bohr-Mottelson (B-M) effective charges are the best. The behavior of the form factors of some Calcium isotopes was studied by using Bohr-Mottelson (B-M) effective charges.
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Three hundred and twelve (312) local fungal isolates were isolated from sixty four (64) different contaminated soil samples with oil wastes at different periods, using potato dextrose agar (PDA).the fungal isolates were tested for its ability to degrade naphthalene .Primary and secondary screening were done using solid (MSM) and liquid (MSM) with 100ppm naphthalene and pH 7 respectively. Results from Primary screening showed that 25 isolates gave good growth, 47 gave moderate growth, 66 gave weak growth and 174 were never growing. According to above results 25 fungal isolates were tested for its ability to degredade naphthalene using liquid mineral media (MSM) pH7,100ppm naphthalene and incubated at 30 0C 120rpm for 7 days. Reduction of
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The study involved 120 women, who were distributed into two groups of breast tumor patients (30 malignant and 30 benign) and a group of controls (60 women). The patients were referred to the Center for Early Detection of Breast Tumor at Al-Alwayia Hospital for Gynecology and Obstetrics (Baghdad) during the period June-December 2011. They were investigated for the frequency of ABO blood group phenotypes, menopausal status, oral contraceptive use, body mass index and family history of breast cancer or other cancers. The results demonstrated that 60.0% of malignant cases clustered after the age 50 years, while it was 20.0% in benign cases. Fifty percent of malignant breast tumor patients reached menopause, while in benign cases, the corresp
... Show MoreThe main aim of this study is to investigate the ability of four local entomopathogenic isolates Beauveria bassiana (Bals.) and Metarhizium anisopliae (Met.) to control the mosquito larvae in the lab. The results revealed that the isolate (MARD48) B .bassiana reduced the survival rate of the mosquito larvae to (80%) followed by the isolate M. anisopliae (MARD10) to (90%) in the first two days of treatment, and 60 and 66% respectively in the third day. The results also showed that the isolate B. bassiana (MARD48) killed 50% of the population (LC50) with the concentration 1×104 conidia/ml compared to 1×107 conidia/ml for the isolates B. bassiana (MARD14) and M. anisopliae (MARD10), and 1×108 conidia/ml for the isolate B .bassiana (MARD76).
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The proton momentum distributions (PMD) and the elastic
electron scattering form factors F(q) of the ground state for some
even mass nuclei in the 2p-1f shell for 70Ge, 72Ge, 74Ge and 76Ge are
calculated by using the Coherent Density Fluctuation Model (CDFM)
and expressed in terms of the fluctuation function (weight function)
|F(x)|2. The fluctuation function has been related to the charge
density distribution (CDD) of the nuclei and determined from the
theory and experiment. The property of the long-tail behavior at high
momentum region of the proton momentum distribution has been
obtained by both the theoretical and experimental fluctuation
functions. The calculated form factors F (q) of all nuclei under s
Background: Candida albicans is the principal fungal infectious agent in human infection. Adhesion is thought to be an essential step for colonization and establishment of Candida infections.
Objectives: Identification and comparison of ALS1 virulence gene of adhesion family among different isolates of Candida albicans by PCR.
Patients and methods: One hundred eight samples were collected from different group of Iraqi patients. All samples were culture on Sabouraud′s agar, CHROMagar for identification while API Candida kit confirmatory test and extracted DNA was done for just Candida albicans isolates, detected the ALS1 gene, extracted RNA for synthesis of cDNA and detected of gene and compare between iso
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The plant Zizyphus spina-christa grows wildly in the middle and southern of Iraq locally named Nabag. In this study the antibacterial activity of several different plant extract (alcoholic hot and cold extract 80%, aqueous hot and cold extract) was tested against some gram negative bacteria that related to Enterobacteriacea as follow; Pseudomonas aeruginosa, Escherchia coli Proteus mirabilis, Serratia mercesence,. Aeromonas sp, Klebsiella pneumoniae ,Shigella sp, Salmonella enteritidis (134), S. typhi(97), S. typhimurium (300) , S. typhi, . The results showed that efficient method of extract was alcoholic hot extract from other extract methods that are used in this study. The detection of active compound in crude extracts of the leaves show
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