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THE GENETIC DIAGNOSIS OF THE BACTERIA ISOLATED FROM THE AGRICULTURAL SOIL SUSTAINED FARMS BY THE POLYMERASE CHAIN REACTION TECHNIQUE QPCR
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The measurements and tests of the samples conducted in the laboratories of the College of Agriculture included isolating bio-fertilizers and testing the efficiency of isolates that fix atmospheric nitrogen and solubilize phosphorous compounds. Bacteria were isolated and identified from the rhizosphere soils of different plants collected from various agricultural areas. A total of 74 bacterial isolates were obtained based on the phenotypic characteristics of the developing colonies, as well as biochemical and microscopic traits. The results of isolation and identification showed that among the 74 bacterial isolates, there were 15 isolates of A. chroococcum, 13 of Az. lipoferum, 13 of B. megaterium, 10 of P. putida, 10 of Actinomycetes, and nitrifying bacteria (Nitrosomonas and Nitrobacter) with 7 and 6 isolates respectively. Bacillus isolates demonstrated the ability to dissolve phosphate compounds with a dissolution diameter ranging between 6-11 mm. The results of the polymerase chain reaction (qPCR) examination confirmed the diagnostic results using the quantitative polymerase interaction technique after extracting DNA from bacterial isolates from the soil. The first group of 10 isolates of P. putida showed, when the gene (GltA F, GltAR) was amplified, resulting bundles with a molecular size of 288 base pairs for P. putida bacteria, confirming the diagnosis using molecular, biological, microscopic, and phenotypic tests. This match confirms the accuracy of the classification of P. putida bacteria. The second group of 10 isolates of P. putida showed, when the gene (PR o DF, PRp o DR) was amplified, resulting bundles with a molecular size of 478 base pairs for B. megaterium, confirming its classification. The third group of 10 isolates of bacteria showed, upon gene amplification (N i f HF, N i f HR), resulting bundles with a molecular size of 371 base pairs for A. chroococcum bacteria, confirming their identification. The fourth group of 14 isolates showed, when the gene (16sAZ F and 16sAZ R) was amplified, resulting bundles with a molecular size of 646 base pairs for Az. lipoferum bacteria, confirming its classification. The fifth group did not show any type of nitrogen-fixing bacteria or phosphate solubilizers after amplification, based on the Sr DNA 71 genotype sequence

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Publication Date
Sat Jun 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
Diagnosis of factors affecting the strategic implementation of sewage projects ( Case study in General Directorate of sewage projects )
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  This  Research aim to identify the factors affecting the strategic implementation of sewage projects and to seek to activate the real follow-up of projects to identify the factors that accompany their implementation, The study included a sample of the projects of the investment plan implemented for the Directorate General of sewage in the governorates of Iraq, which was completed during the six years period (2010-2016). The sample of the research was four projects: The project of implementation and processing of the treatment plant and the lifting station and the conveyor line for the project of IMARA/The third stage/Al-Sanaf marshland , The project of the processing and implementation of the treatment plant with the

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Publication Date
Thu May 02 2019
Journal Name
University Of Anbar Sport And Physical Education Sciences
The Effect of the Exercises of Retrieving the ball from the Net on the Development of the skill of Defending the stadium in the Volleyball
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QA Sarhan, University of Anbar Sport and Physical Education Sciences, 2019

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Lactic Acid Bacteria (LAB) with Probiotic Potential from Dengke Naniura
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Dengke Naniura is a traditional food from Sumatera Utara, Indonesia, that is produced through fermenting process, and this food is believed to contain high probiotics. The objective of the current research is to determine the potential of LAB as a probiotic that has been obtained from Dengke Naniura. Dengke Naniura was traditionally prepared from Cyprinus carpio. Four LABs have been successfully isolated from Dengke Naniura, such as D7DA3, D7B3, D7DBF and D7DN3. Those four LAB isolates were identified as Lactobacillus sp. This result has been confirmed by the non-spore forming bacterium, non-motile, and Gram-positive. Also, it has been supported by biochemical test, for the example Voges Proskauer, catalase test, Methyl

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Publication Date
Fri Dec 31 2021
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
PHYSIO-CHEMICAL ANALYSIS OF SAMPLES OF SOIL SELECTED FROM DIFFERENT AGRICULTURE AREAS FROM AL-NAJAF: PHYSIO-CHEMICAL ANALYSIS OF SAMPLES OF SOIL SELECTED FROM DIFFERENT AGRICULTURE AREAS FROM AL-NAJAF
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The twelve samples of agricultural soils from four regions in Al-Najaf governorate with sampling plant with soil. Physical properties of the soil where studied, such as electrical conductivity ranged from (136.33-1070.00)μS/cm-3, and moisture which ranged between the values (0.39-36.48)%. The chemical analysis of the soil have included the proportion of calcium carbonate the ratio between (44.00-48.00%) has been observed increasing amounts of calcium carbonate in surface models. The pH where results indicate that pH values ​​were close to study models ranged between (6.88-7.42) these values ​​generally within the normal range for the measured pH values ​​of the Iraqi soil. The amount of gypsum ranged betwe

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Publication Date
Wed May 17 2023
Journal Name
College Of Islamic Sciences
The methods used by the Prophet in teaching the Companions
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The condition of Islam and Muslims and what their political, social, cultural and educational status, and even religious and faith status, has led to whoever feels his belonging to this great religion and that middle nation, to try as much as he can to fix what has been corrupted as much as he can, and perhaps in diagnosing illness and illness as It was said half the way to treatment, and from this standpoint I liked to occupy thought, work consideration and harness part of the youth’s life in order to reach what contributes to reforming the situation and the safety of generations, and this is only in seeking knowledge and learning it by following the guidance of the Messenger (r) and his companions ( y) Therefore, the subject of my re

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Publication Date
Sun Jun 03 2012
Journal Name
Baghdad Science Journal
Studying Proteus mirabilis Typing Isolated from Urinary Tract infections
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Urine specimen (253 samples) had been collected from urinary tract infections. The study showed that Proteus mirabilis was responsible for (11.85%) of the urinary tract infections. Also, the study had declared that the ratio of isolation of this bacterium from women was (7.51%) and it was higher than the ratio in both men and children which ranged (1.58%) and (2.76%) respectively. Morphological and biochemical studies had been applied to characterize this bacterium as well as other kinds of microorganisms that were isolated from urinary tract infection in this study. The study deals with typing methods such as using biotyping and typing according to Dienes phenomenon beside the succestibility to antibiotics. The results had shown that the s

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Publication Date
Fri Dec 31 2021
Journal Name
Journal Of Communicable Diseases
The Relation between RT-PCR and Chest CT Scan Sensitivity as Tools in the Diagnosis of COVID-19 Patients in Al-Najaf Province, Iraq
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The typical test for diagnosis of severe acute respiratory syndrome coronavirus 2 is a reverse transcription-polymerase chain reaction (RT-PCR) technique, but the chest CT scan might play a complementary role at the first detection of Coronavirus Disease 2019 (COVID-19) pneumonia. Objectives: To determine the sensitivity of CT scan on patients with COVID-19 in Al-Najaf, Iraq, and to compare the accuracy of CT scan with that of RT-PCR technique. Material and Method: This is a prospective study. The patients suspicious of having COVID-19 infection and respiratory symptoms were registered. All patients were diagnosed by RT-PCR and chest CT. Diagnostic performance of CT was intended using RT-PCR as the reference sta

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Publication Date
Mon Mar 13 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Spectrophotometric Determination of Paracetamol by Diazotization and Coupling Reaction with Anthranilic Acid
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This paper concerned with development of a spectrophotometric method for the determination of paracetamol, based on the diazotisation and coupling reaction with anthranilic acid in basic medium, to form an intense yellow coloured, water-soluble and stable azo-dye which shows a maximum absorption at 421nm. Beer’s law is obeyed over the concentration range of 1.0-10 µg/ml; with molar absorptivity of 2.1772×104 L.mol -1.cm-1 and Sandell’s sensitivity index 6.9446 µg.cm-2. The method has been applied successfully for the determination of paracetamol in pharmaceutical formulation. 

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Publication Date
Sun Mar 17 2019
Journal Name
Baghdad Science Journal
Spectrophotometric Determination of Chlorpromazine Hydrochloride Using 4-Nitroanilline by Oxidative Coupling Reaction
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A simple, rapid spectrophotometric method has been established for the determination of chlorpromazine hydrochloride (CPZ) in its pure form and in a tablet formulations. The  suggested  method  is  based  on  the  oxidative coupling  reaction  with4-nitroainlline using KIO3 in acidic solution to produce a violet colored product with maximum absorption at λ=526 nm.The  analytical data  obtained  throughout  this  study  could  be  summarid  as  follows:  1ml of 1M HCl (pH=2.2), 1 ml  of  4-nitroanilline (1x10-2M), and 1.5ml  of (1x10-2)KIO3 per 25 ml reaction medium. The order of a

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Publication Date
Fri Jan 01 2021
Journal Name
Desalination And Water Treatment
Electrosorption of cadmium ions from the aqueous solution by a MnO2/carbon fiber composite electrode
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The nanostructured MnO2 /carbon fiber (CF) composite electrode was prepared using the anodic electrodeposition process. The crystal structure and morphology of MnO2 particles were determined with X-ray diffraction and field-emission scanning electron microscopy. The electrosorptive properties of the prepared electrode were investigated in the removal of cadmium ions from aqueous solution, and the effect of pH, cell voltage, and ionic strength was optimized and modeled using the response surface methodology combined with Box–Behnken design. The results confirm that the optimum conditions to remove Cd(II) ions were: pH of 6.03, a voltage of 2.77 V, and NaCl concentration of 3 g/L. The experimental results showed a good fit for the Freundli

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