The application of physiological oxygen (physoxia) concentrations is becoming increasingly commonplace within a mammalian stem cell culture. Human mesenchymal stem cells (hMSCs) attract widespread interest for clinical application due to their unique immunomodulatory, multi-lineage potential, and regenerative capacities. Descriptions of the impact of physoxia on global DNA methylation patterns in hMSCs and the activity of enzymatic machinery responsible for its regulation remain limited. Human bone marrow-derived mesenchymal stem cells (BM-hMSCs, passage 1) isolated in reduced oxygen conditions displayed an upregulation of SOX2 in reduced oxygen conditions vs. air oxygen (21% O2, AO), while no change was noted for either OCT-4 or NANOG. DNA methylation marks 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) showed decreases in 2% O2 environment (workstation) (2% WKS). DNMT3B (DNA methyltransferase 3B) and TET1 (Ten-eleven translocation enzyme 1) displayed reduced transcription in physoxia. Consistent with transcriptional downregulation, we noted increased promoter methylation levels of DNMT3B in 2% WKS accompanied by reduced DNMT3B and TET1 protein expression. Finally, a decrease in HIF1A (Hypoxia-inducible factor 1A) gene expression in 2% WKS environment correlated with protein levels, while HIF2A was significantly higher in physoxia correlated with protein expression levels vs. AO. Together, these data have demonstrated, for the first time, that global 5mC, 5hmC, and DNMT3B are oxygen-sensitive in hMSCs. Further insights into the appropriate epigenetic regulation within hMSCs may enable increased safety and efficacy development within the therapeutic ambitions.
Studying translation as academic article has cultural motive side by side academic aim to establish a professional generation that have the ability to serve labor market .
And because translation is important in many sectors in the society such as technology, science, popular institutions, tourism and political and legal relations between countries.
Our investigation concentrate on the articles of translation teaching in fourth class/ Spanish Department/ College of Languages/ Baghdad University for five years in order to evaluate the level of utility of creating translators and interpreters after graduation .
From 2009 until 2014 we saw continuous yearly exchanging in teaching articles by many professors with diff
... Show MoreThis research focuses on shedding light on the most important criteria by which the translator can achieve total success with regard to the translation of scientific texts whose written language in Spanish is greatly influenced by expressions and words adopted from the English language and other world languages, in addition to exposing the most outstanding difficulties that translators face when translating from Spanish to Arabic, giving the most appropriate solutions in this regard.
Resumen
El presente trabajo de investigación se centra en echar luz sobre los criterios más importantes por los cuales el traductor puede conseguir el total éxito en la traducción de los textos científicos cuyo lenguaje
... Show MoreEl español es una lengua hablada por una ingente comunidad que abarca varios cientos de millones de personas. Son muchas las palabras con distintos significados que se escriben de similar manera. Hablamos, por ejemplo, de dos de ellas: el adjetivo solo y el adverbio sólo.
En este trabajo se plantea un estudio del adverbio sólo, palabra invariable cuya función consiste en (complementar la significación del verbo) y solo es un adjetivo (califica a un sustantivo).¿Cómo diferenciarlas para evitar errores ortográficos? Así, en estos casos (solo/ sólo), la distinción la da la tilde en la primera vocal. Ésta es la
... Show MoreAbstract. Shock chlorination is a well-known practice in swimming pools and domestic wells. One of the limitations for using this technique in drinking water purification facilities is the difficulty of quickly removing high chlorine concentrations in water distribution systems or production facilities. In order to use this method in the drinking water industry a shock de-chlorination method should be introduced for producing microorganism and biocide free water. De-chlorination using natural stagnant aeration (leaving the water to lose the chlorine naturally) is the safest known method if compared with chemical and charcoaling methods. Unfortunately, stagnant aeration is a slow process. Therefore, developing a process for accelerat
... Show MoreWriting his own biography is like making his confession in front of the altar of the truth. In The words,Sartre writes his own child life from the view of a philosopher-thinker. He presents his days spent justlike a series of images carrying advices and experiencesto whole the world. The words shows the life of a small orphan who met early with death when he lost his father. Then death becomes as his shadow that goes with him on day and night until the end. The mother of the little Poulou seems like the mirror that reflects the sadness and the melancholy of the dark and gloomy days. The small Poulou becomes his only consolation in misery. The little Jean -Paul lives completely in a literary atmosphere . Because his grandfather Karl Schwe
... Show MoreIn our research, we seek to shed a light on one of the most important and sensitive issues, namely, the Sufi influence in the Iraqi novel through the lame maqam of the novelist Jumaa Al-Lami, the Sufi discourse contains many semantic paradoxes between the text's apparent pronunciation and its interpretation of the format and the context that produced these patterns, and incited them, which concludes different results from the prevailing provisions and fixed ideas from the narrative text.The Arabic and Iraqi novel in particular became inspired by the power of Sufi discourse by talking about several Sufi figures by referring to it openly, or implicitly inspired by unauthorized concealment, in employing some of the ideas, or summoning
... Show MoreAbstract Aim: Autism is a neurodevelopmental disorder which affects communication and social interaction of children. It is a heterogeneous disease with various clinical presentations. Some genes are involved in its pathogenesis. It has been suggested that environmental exposure to lead can increase the risk of autism. The aim of our study was to compare blood lead levels among autistic and non-autistic children. Material and Method: This retrospective study included 107 children (60 with autism and 47 without autism) referred from the different Iraqi provinces, in the years 2015, 2016 and 2017, to the poisoning consultation center in Baghdad. Data collection including age, gender, residence, referral source, family history and blood lead l
... Show MoreAll foreign language teachers highlight the difficulties students face during learning. The aim of this work is to explain the most complex problems of teaching foreign language grammar to Spanish language students at the University of Baghdad. Its methodology is based on an assessment of a group of intermediate-level students in the Faculty of Languages at the University of Baghdad. Furthermore, the responses are analyzed, and the errors are described. We conclude that the best method for teaching the passive voice to students is based on an analysis from syntactic and morphological perspectives. We present the main distinguishing characteristics of the Spanish language to students and offer some recommendations for learning Spanish
... Show MoreResearch Summary :
Praise be to God, Lord of the Worlds, and prayers and peace be upon the Master of the Messengers, his family and all his companions, then after:
This is a brief research that contained its two rudders the command and its Style in Surat Al-Ma’idah a fundamental rhetorical study, and the study clarified the meaning of imperative in both; the Arabic language and in the terminology of the fundamentalists and rhetoricians in a concise manner, and then indicated the imperative of the command , the true meaning, and the meanings interpretated as an imperative form. I have mentioned some verses of Surat Al-Ma’idah, so what I have quot
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