Background: Premature infant born with immature body system, their organs are not ready for extra uterine life, and they are unable to deal with external stress, which could alter body functions such as cardio-respiratory function. In addition, poor muscle tone increases the chance of developing an abnormal posture. To reduce this instability, applying developmental care such as nesting is vital to promote cardio-respiratory stability, maintain position, and reduce stress in preterm.      Objectives: The study aims to assess the impact of the nesting technique on preterm cardio-respiratory parameters in various positions (supine, prone, and right lateral).       Methodology: The research used randomized controlled trial design. By simple random sampling technique was choose 60 preterm infant, and they were divided into four groups equally (supine, prone, right lateral, and control group). The cardio-respiratory parameters (heart rate, respiratory rate, and oxygen saturation) were measured before and after applying the nesting and positioning techniques for 3 consecutive days. Data were analyzed by Statistical Package for Social Sciences (SPSS) program.                                                                                  Results: The study results showed a significant difference in the mean score of cardio-respiratory after three days of nesting technique with lateral, supine, and prone position groups at p= 0.000. While the control group showed there was non-significant change in the mean score of HR, RR, and Spo2 at p > 0.05.          Conclusion: The Study concluded that the nesting technique was effective for stabilizing the cardio-respiratory parameters of preterm infants. The authors recommended routinely using nesting with the ideal position technique in preterm as a part of  developmental care at the neonatal intensive care unit