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Molecular detection of fimH& mrkDgenes of strong biofilm producers & MDR Klebsiella pneumoniae
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Klebsiella pneumoniae is an adaptable pathogen that forms biofilms on a variety of surfaces. This study's objective was to identify the presence of fimbrial genes (types 1 and 3) in K. pneumoniae strains isolated from various clinical sources based on their antibiotic resistance and ability to form biofilms. According to identification utilizing the vitek 2 technology and confirmation by molecular identification targeting the 16S rRNA gene with a particular primer, forty isolates were identified from clinical specimens. The vitek 2 compact system was utilized to evaluate the antibiotic susceptibility of all the isolates. The findings revealed a range of resistance percentages, including 52.5% for Penicillin, 40.5% for Trimethoprim/Sulfamethoxazole, 34.5% for Cephalosporins, 6.25 % for Fluoroquinolones, and 2.5% for each of Carbapenem, Aminoglycoside, Tetracycline, and Nitrofurantoin. The 96-well microtiter plate technique was utilized to generate biofilms. The results demonstrated that all 40 Klebsiella pneumoniae isolates (100%) produced potent biofilms. In order to identify the genes involved in biofilm formation (fimh & mrkd) and the genes responsible for adhesin in type 1& type 3 fimbriae using traditional PCR method, eleven isolates were chosen for molecular analysis that are powerful biofilm makers and MDR. 

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Publication Date
Mon Jan 29 2024
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )
Role of Ultrasound in Antenatal Detection of Echogenic Amniotic Fluid and Pregnancy Outcome
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Background: Ultrasound is a valuable tool for evaluating fetal problems throughout pregnancy. Amniotic fluid anomalies have been associated with unfavorable maternal, fetal, and obstetrical outcomes. Objective: To determine the effect of echogenic amniotic fluid during term pregnancy on the presence of meconium stain liquor and pregnancy outcome. Methods: A cross-sectional study was conducted on 1080 term pregnant women who visited Al-Elwiya Maternity Teaching Hospital from May 1st, 2021, to May 1st, 2023. Ultrasound was used to analyze echogenic amniotic fluid and turbid liquor. The liquor state was tested either after an artificial membrane rupture in the vaginal delivery trial or during a cesarean section. Results: Echogenic amni

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Publication Date
Wed Nov 27 2019
Journal Name
Iraqi Journal Of Science
An Improved Segmentation Technique for Early Detection of Exudates of Diabetic Retinopathy Disease
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Diabetic retinopathy (DR) is a diabetes- caused disease that is associated with  leakage of fluid from the blood vessels into the retina, leading to its damage. It is one of the most common diseases that can lead to weak vision and even blindness. Exudates is a clear indication of diabetic retinopathy, which is the main cause of blindness in people with diabetes. Therefore, early detection of exudates is a crucial and essential step to prevent blindness and vision loss is in the analysis of digital diabetic retinopathy systems. This paper presents an improved approach for detection of exudates in retina image using supervised-unsupervised Minimum Distance (MD) segmentation method. The suggested system includes three stages; First, a

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Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Detection of mexB Multidrug Efflux Gene in Some Local Isolates of Pseudomonas aeruginosa
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      According to the prevalence of multidrug resistance bacteria, especially Pseudomonas aeruginosa, in which the essential mechanism of drug resistance is the ability to possess an efflux pump by which extrusion of antimicrobial agents usually occurs, this study aims to detect the presence of mexB multidrug efflux gene in some local isolates of this bacteria that show resistance towards three antibiotics, out of five. Sensitivity test to antibiotics was performed on all isolates by using meropenem (10µg/disc), imipenem (10µg/disc), amikacin (30 μg/disc), ciprofloxacin (5µg/disc) and ceftazidime (30 µg/disc). Conventional PCR results showed the presence of mexB gene (244bp) in four isolates out of t

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Publication Date
Tue Mar 30 2021
Journal Name
Iraqi Journal Of Science
Detection of Listeria monocytogenes in Several Types of Frozen Meat in Baghdad city
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Detection of pathogenic bacteria, such as Listeria  monocytogenes, in food is crucial for safeguarding public health in Iraq.  Forty five samples of frozen meat (15 samples of each of minced red meat, chicken, and fish) were collected from different markets in Baghdad city. Molecular (RT-PCR) and culturing (conventional microbiological examination) methods were used to determine the level of contamination of L. monocytogenes in these types of meat.

For the culturing method, TSYEB broth was used as an enrichment medium, whereas BALCAM medium (HiMedia) with the listeria selective supplement FD061 was used as a selective medium, for the isolation and identification of this bacterium. The isolates were con

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Publication Date
Sun Aug 30 2020
Journal Name
Journal Of Economics And Administrative Sciences
The importance of using analytical procedures in the detection of creative accounting practices
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The research aims to identify the importance of using analytical procedures in the detection of creative accounting practices. To achieve this goal, (100) questionnaires were prepared and distributed to the auditors in the Federal Financial Supervision Bureau and the authorized auditors' offices and practitioners of the auditing profession in Iraq. For the purpose of testing the research hypothesis and analyzing data, some appropriate statistical methods have been used and the use of the statistical program (SPSS) to analyze the data. The results of the research showed that the analytical procedures and tests applied by the auditor have a role in revealing and limiting creative accounting practices and methods and that auditors u

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Publication Date
Wed Aug 31 2022
Journal Name
Iraqi Journal Of Science
Accumulation Detection of Cadmium in some land-use soil of Baghdad city, Iraq
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    This study aims to detect cadmium accumulation in the soil of Baghdad. Twenty soil samples were collected randomly during November 2020 to cover the study area, emphasizing the nature of each area (agricultural, commercial, industrial, residential, roadside, and waste dumping sites). All soil samples were subjected to geochemical analysis using atomic absorption spectrometry (ASS) to determine the concentration of cadmium in Baghdad soil. The laboratory data was utilized to design the spatial analysis map using Arc GIS 10.4.1 to investigate the spatial distribution of cadmium. The results demonstrated that the total content of cadmium in the study area ranged from 0.121to 1.78 mg/kg. All results of cadmium concentrations are withi

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Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Detection of mexB Multidrug Efflux Gene in Some Local Isolates of Pseudomonas aeruginosa
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According to the prevalence of multidrug resistance bacteria, especially Pseudomonas aeruginosa, in which the essential mechanism of drug resistance is the ability to possess an efflux pump by which extrusion of antimicrobial agents usually occurs, this study aims to detect the presence of mexB multidrug efflux gene in some local isolates of this bacteria that show resistance towards three antibiotics, out of five. Sensitivity test to antibiotics was performed on all isolates by using meropenem (10μg/disc), imipenem (10μg/disc), amikacin (30 μg/disc), ciprofloxacin (5μg/disc) and ceftazidime (30 μg/disc). Conventional PCR results showed the presence of mexB gene (244bp) in four isolates out of ten (40%). In addition,25, 50μg/ml of cur

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Publication Date
Thu Jul 01 2021
Journal Name
Iraqi Journal Of Science
The Efficiency of Apple Vinegar as A Solvent in Comparison to Water and Ethanol for The Extraction of Some Plants used Against Candida Spp. Biofilm Formation
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Apple vinegar has many uses that include burn and wound healing and as an antimicrobial agent against different microorganisms, but not as a solvent. Therefore, this study aimed to use commercial apple vinegar as solvent to the plants of  roselle (Hibiscus sabdariffa), green tea (Camellia sinensis), and clove (Syzygium aromaticum). The effects of apple-vinegar extracts of these plants were compared with those of aqueous and ethanolic extracts against biofilm formation by Candida genus. Clove vinegar extract demonstrated antibiofilm activity against C. albicans, alone (2.4907± 0.382) or in combination with the antifungal agents fluconazole (1.689±0.33), nystati

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Publication Date
Mon Jan 01 2024
Journal Name
Pharmaceutical Sciences Asia
Effect of sub-minimum inhibitory concentrations of ceftriaxone on the Pseudomonas aeruginosa adhesion to human oral mucosal epithelial cells and biofilm formation to polystyrene in vitro
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Publication Date
Thu May 28 2020
Journal Name
Iraqi Journal Of Science
Morphological and Molecular Identification of Three Genera of the Family Heptageniidae (Ephemeroptera) from Ava Sheen Branch/ Greater Zab Tributary, North of Iraq
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The aquatic Heptageniidae family of Ava-sheen branch (Greater Zab Tributary) in Duhok Governorate/ Iraq was studied. Samples were collected using Surber stream sampler to study their diagnostic morphological characteristics and molecular phylogenetic profile using a nuclear gene 16s ribosomal RNA. The morphological and molecular identification supported that the three species, Epeorus longimanus, Heptagenia elegantula and Ephemerella cornutus belong to Heptageniidae family, while the molecular results also confirmed the monophyletic origin of these three genera.

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