The members of the family of Eentrobacteriaceae harbour a gene cluster called polyketide synthase (pks) island. This cluster is responsible for the synthesis of the genotoxin colibactin that might have an important role in the induction of double-strand DNA breaks, leading to promote human colorectal cancer (CRC). Eleven out of the eighty eight isolates (12.5%) were pks+, distributed as 7 (8%) isolates of E. coli, 2 (2.25%) of K. pneumoniae and 2 (2.25%) of E. aerogenes. The cytotoxic effects of selected pks+ isolates (E. coli and E. aerogenes) on HeLa cells were represented by decreasing cell numbers and enlarged cell nuclei in comparison to the untreated cells. Cyt
... Show MoreThe members of the family of Eentrobacteriaceae harbour a gene cluster called polyketide synthase (pks) island. This cluster is responsible for the synthesis of the genotoxin colibactin that might have an important role in the induction of double-strand DNA breaks, leading to promote human colorectal cancer (CRC). Eleven out of the eighty eight isolates (12.5%) were pks+, distributed as 7 (8%) isolates of E. coli, 2 (2.25%) of K. pneumoniae and 2 (2.25%) of E. aerogenes. The cytotoxic effects of selected pks+ isolates (E. coli and E. aerogenes) on HeLa cells were represented by decreasing cell numbers and enlarged cell nuclei in comparison to the untreated cells. Cytological changes were observed when the infected HeLa cells culture
... Show MoreThis study was conducted at the College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad. The aim of this study was to isolate and diagnose fungi from fish feedstuff samples, and also detection of aflatoxin B1 and ochratoxin A in fish muscles and feedstuffs. Randomly, the samples were collected from some fish farms from Baghdad, Babil, Wasit, Anbar, and Salah al-Din provinces. This study included the collection of 35 feedstuff samples and 70 fish muscle samples, and each of the two fish samples fed on one sample of the feedstuff. The results showed the presence of several genera of different fungi including Aspergillus spp, Mucor spp., Penicillium spp., Yeast spp., Fusarium spp., Rhizopus spp., Scopiolariopsis spp., Ep
... Show MoreThe aim of this study was to isolate and identify the cyanobacterium Scytonema hofmanni Var. calcicolum from the domestic drinking tanks as a new record in Iraqi drinking water. Scytonema hofmanni var. calcicolum, a filamentous freshwater cyanobacterium (blue-green alga). This alga was isolated from the walls of the domestic plastic water tanks in Al- karkh/ Baghdad city on July 2014. The sampling was performed by collecting three samples from this tanks, the three examined samples microscopically revealed the dominance of this cyanobacterium as unialgal in the studied samples. The results showed this alga has the ability to tolerate high temperature up to 42 Cº and very low light intensity inside the tanks which up to 10 μE/m²/s.
Background: The skin functions as a barrier to the external environment, damage to this barrier following a burn disrupts the innate immune system and increases susceptibility to bacterial infection. Objective: This study was carried out to determine the bacterial isolates and study their antimicrobial susceptibility in burned wound infections at one burn's hospital in Baghdad.Type of study:Cross-sectional study.Methods: The bacteria were identified at species level by using Analytic Profile Index (API) system and The antimicrobial susceptibility test was performed according to Kirby-Bauer (disk diffusion) technique.Results: Over a period of one year (from October 2014 to October 2015). Out of 848 patients with different degrees of burns
... Show MoreBackground: Maxillary sinusitis is one of the most common infections of humans. Sinusitis can be defined as an inflammation of the membrane lining of any sinus, especially one of the
paranasal sinuses.
Objective: To determine the causative microorganisms of chronic maxillary sinusitis.
Patients: Forty five chronic sinusitis patients were involved in the present study.
Methods: Sampling method were sinus specimens (aspiration or injection aspiration).
Results: Haemophilus species, Streptococcus pneumoniae (S.pneumoniae) and Moraxella catarrhalis (M.catarrhalis) were the most frequent isolates; in addition Penicillium and
Cladosporium species were isolated from some chronic sinusitis patients.
Conclusion: Chr
Background: Maxillary sinusitis is one of the most common infections of humans. Sinusitis can be defined as an inflammation of the membrane lining of any sinus, especially one of the
paranasal sinuses.
Objective: To determine the causative microorganisms of acute maxillary sinusitis.
Patients: Forty five acute sinusitis patients were involved in the present study.
Methods: Sampling methods were per-oral nasopharyngeal swabs.
Results: Haemophilus species, Streptococcus pneumoniae (S.pneumoniae) and Moraxella catarrhalis (M.catarrhalis) were the most frequent isolates.
Conclusion: The most causative agents of acute maxillary sinusitis were bacterial isolates, which were Haemophilus species followed by S.pne
313 blood samples were collected from bacteremia patients, including 146 samples (30 from patients and 116 from outpatients) from Azadi teaching hospital, 36 samples from the dialysis unit at Kirkuk General Hospital, 126 samples (42 from inpatients and 84 from outpatients) from the Children's Hospital, and 5 samples from the Women's and Obstetrics Hospital in Kirkuk province, for the period from January 24, 2022, to September 10, 2022. The study, including the isolation and diagnosis of bacteria and the study of their resistance to antibiotics, The results show that 32 (17.87%) positive growth cultures were obtained from febrile patients, 3 (8.33%) from dialysis patients in the dialysis unit, and 15 (65.21%) from burn and wound patients.
... Show MoreDiatoms are considered a potentially new and valuable source of biologically active compounds including those with antimicrobial properties; so, this study was conducted to evaluate the antibacterial activity of Navicula incerta. The diatom was isolated from the salt water of Sawa Lake, southern of Iraq, it was cultivated in salt water then was adapted and cultivated in freshwater environment; the harvested and dried biomass was extracted, and the antibacterial activity of each extract was evaluated against several species of pathogenic bacteria. The chemical constituents of the extracts were also analyzed using Gas chromatography/Mass spectrometry technique. Generally, the result showed that fresh water extract of N. incert
... Show MoreThe present study was carried out to evaluate antibacterial activity of water , alcoholic extracts (cold and hot) and the crude alkaloid extract of leaves, seeds and roots of Zygophyllum fabago plant against the growth of some bacteria including gram negative bacteria(Escherichia coli ,Pseudomonas aeruginosa) and gram positive bacteria(Staphylococcus aureus ,Bacillus subtilis). Results showed variation in antibacterial activity of different extracts against the studied bacteria. Hot alcoholic extracts showed the highest antibacterial activity followed by hot water extract. Additionally, alcoholic extracts of seeds revealed a higher activity compared with other plant extract.Results of sensi
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