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Investigation of pathogenic Streptococci species in local and imported mozzarella soft cheese in Baghdad city
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Background: The present work investigated the profile and biodiversity of the pathogenic streptococci species isolated from local and imported mozzarella soft cheese in Baghdad City from October 2022 to January 2023. The study aimed to examine the molecular characterization of 16Sr RNA gene in some streptococcus species isolates from mozzarella soft cheese in Baghdad city Methods: From 50 samples, 8 isolates in all were found and identified based on the VITEK, molecular, and sequencing of the 16SrRNA gene. The eight isolates represented Lactococcus cremoris. Streptococcus alactolyticus, Streptococcus sanguinis, and Streptococcus thoraltensis. The isolates were subjected to conventional PCR and electrophoresis to detect the 16SrRNA gene using specific primers , Streptococcal genes were used in the neighbor-joining phylogenetic analysis that determined these sequences were derived from Streptococcal genes. Results: DNA sequencing evaluation Significant alignments (96-99% identities) to the Streptococci isolates found in BLAST-NCBI Gene-bank were discovered after sequencing the bacterial DNA products generated by PCR. The results revealed that 8/50 amplified the 16SrRNA gene, which has a molecular weight of about 1250 bp. the gene distribution was statistically significant (p ≤ 0.05). Conclusion: Streptococcus isolates from mozzarella soft cheese by using molecular tools to first stop the spread of the epidemic of harmful microorganisms in food, it is represented a health risk for the consumers.

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Publication Date
Tue Jun 30 2015
Journal Name
Al-kindy College Medical Journal
Symptoms profile of patients with major depression in Baghdad
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:Background: Many studies had stated that there are marked variations in the clinical presentation of depressive states between different cultures.Objectives: The main aim of the study is to identify the symptoms profile of patients with major depressive disorder living in Baghdad.Method: Ninety two patients with major depressive disorder consulting Ibn-Rushd psychiatric teaching hospital were studied thoroughly to identify the frequency of symptoms among them. The fifth edition of the Arabic version of the Mini-International Neuropsychiatric Interview (M.I.N.I) was used to identify the Diagnostic and Statistical Manual – fourth edition (DSM-IV) symptoms. A list of other symptoms, which were found to present variably in depression acco

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Publication Date
Thu Sep 09 2021
Journal Name
International Journal Of Research In Pharmaceutical Sciences
Seroprevalence of celiac disease among children in Baghdad, Iraq
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Publication Date
Thu Aug 31 2023
Journal Name
Iraqi Geological Journal
Geochemistry of Sabkhas in Abu Ghraib, Western Baghdad, Iraq
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The study aims to detail the chemistry of Sabkha located in Abu-Graib, western Baghdad to know the content of the rare elements distributed. Sabkhas are found in dry areas with significant evaporation rates. The quantity of dissolved salts rises as water evaporates from the shallow basins, eventually causing salt to crystallize. The creation of the distinctive salt pans and crusts on the top is greatly influenced by this process. The trace elements (Ti, V, Cr, Mn, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Y, Zr, Nb, Mo, Sn, Ba, Ta, Tl, Pb, Th, U, I, and Br) beside the major oxides were analyzed using XRF technique. There are no clear significant differences in the distribution of the elements between the two Sabkhas in the village of Kadim Al

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Publication Date
Thu Sep 30 2010
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
CORROSION of BOILER TUBES in SOUTH BAGHDAD ELECTRIC STATION
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The corrosion of low carbon steel boiler tubes in demi water had been investigated. The purpose of this investigation was to determine the change produced in corrosion behavior of the carbon steel as a result of the specific presence of chloride and copper ions in the water under different temperatures. For low carbon steel experiments, the temperature was taken in three levels (125, 175, and 215°C) under about 27 bar pressure and 1500 rpm in autoclave. Using weight loss technique, the corrosion rate ranges from (85 to 789 gmd) for low carbon steel boiler tubes.

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Using PCR for detection of cutaneous leishmaniasis in Baghdad
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Cutaneous Leishmaniasis (CL) is an endemic disease and one of the major health problems in Iraq. Leishmania tropica is known as the causative agent of Cutaneous Leishmaniasis in Baghdad.The classical serological methods of diagnosing leishmaniasis is a poor sensitivity especially for the sub genus and time consuming Here we have investigated two primer pairs, one specific for Leishmania as genus and the primer specific for the species of L. tropica to be detected by polymerase chain reaction (PCR).Samples were collected from (AL-karama Teaching Hospital) and whole genomic DNA was extracted from axenic promastigotes.The extracted DNA was amplified by PCRwith two KDNA primer pairs, for genus specific (13A/13B) and (Lmj4/Uni21) to identify

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Publication Date
Tue Jun 30 2020
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
DETECTION OF HEAVY METALS POLLUTION INTYPES OF MILK SAMPLES IN BAGHDAD MARKETS: DETECTION OF HEAVY METALS POLLUTION INTYPES OF MILK SAMPLES IN BAGHDAD MARKETS
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The levels of lead (pb), copper (cu), cobalt (co) and cadmium (cd) were determined in different kinds of milk and the health risks were evaluated. The mean levels were 0.73±0.21, 0.06±0.01, 0.12±0.01 and 0.14±0.01 ppm for these metals respectively. The levels of pb and cu were found to be insignificant differences (p<0.05), whereas the levels of co and cd, were no significant differences (p>0.05). The dry and liquid kinds of milk were different significantly (p<0.05), whereas the original, was no significant differences (p>0.05). The values for all metals were more than one. The metals pb and cd were detected at highest concentrations in most dry and liquid milk samples.

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Publication Date
Wed May 24 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Investigation of alkaloids of Anabasis aphylla (Chenopodiaceae)
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The aerial part of Iraqi Anabasis aphylla (Chenopodiaceae) had been investigated for its alkaloidal contents.The alkaloid anabasine [2-(3-pyridyl)-piperidine] [1] & aphyllidine were isolated from an ethanolic extract of the plant. Isolation of the alkaloid was done by column chromatography followed by preperative thin layer chromatography. Identification of the isolated alkaloid was done by different spectroscopic methods (UV,IR), refractive index & TLC using authentic sample & preparation of a salt.

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Publication Date
Sun Jun 03 2018
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Investigation of Solubility Enhancement Approaches of Ticagrelor
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ABSTRACT

                   Ticagrelor is an orally administered antiplatelet medicine, direct-acting P2Y12-receptor antagonist. Ticagrelor binds reversibly and noncompetitively to the P2Y12 receptor at a site distinct from that of the endogenous agonist adenosine diphosphate (ADP). Inhibition of platelet aggregation stimulated by ADP is a commonly used pharmacodynamic parameter for P2Y12-receptor antagonists.

                  Ticagrelor is a crystalline powder with an aqueous solubility of approximately 10?g/mL at room temperature.

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Determination local geoid Heights Using RTK-DGPS/Leveling and transformation methods
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Geodesy is concerned with the relative positioning of points and the gravity field of the earth. For this task, a well-defined coordinate system is needed on which measurements are normally tied to a set of reference points called a geodetic datum (geoid or ellipsoid). The Global Positioning System GPS gives accurately the three-dimensional position of a point (latitude, longitude, and ellipsoidal height) and can measure under all weather conditions. The coordinates of the GPS reference to the World Geodetic System1984 (WGS 84), a global ellipsoid having its origin as the mass center of the earth, and height, referenced to the surface of the ellipsoid . In this research , using RTK-DGPS technique Data collection for study local and level

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Publication Date
Sun Mar 01 2009
Journal Name
Baghdad Science Journal
Purification and Characterization &#946; - lactamase produce from local isolate Klebsiella pneumonia
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Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration.Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ? C .During storage the enzyme retained 72% at -20 ? C and retained 25% of the activity at the same period at 4 ? C.

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