Background: Plasma-activated water (PAW) is considered one of the emerging strategies that has been highlighted recently in the food industry for microbial decontamination and mycotoxin detoxification, due to its unique provisional characteristics. Aim: The effectiveness of PAW for aflatoxin B1 (AFB1), ochratoxin A (OTA), and fumonisin B1 (FB1) detoxification in naturally contaminated poultry feeds with its impacts on the feed quality were inspected. Methods: PAW-30 and PAW-60 were utilized for feed treatment for six time durations (5, 10, 15, 20, 40 and 60 min) each. The alterations in the physicochemical properties of PAW after different time durations of plasma inducement and treatment with and without feed samples were monitored. Competitive Enzyme-Linked Immunosorbent Assay (ELISA) was employed for estimation of mycotoxin levels and High Performance Liquid Chromatography (HPLC) was utilized for results confirmation. Feed composition analyses with peroxide values (PVs) estimation were implemented according to standard analytical methods. Results: The physicochemical properties of PAW showed a significant decrease (P<0.05) in pH value from 6.72 to 2.68 and a significant increase (P<0.05) in oxidation-reduction potential (ORP), electrical conductivity (EC) and temperature from 235 mV, 5.1 μS/cm and 20.5 °C to 499.2 mV, 727.6 μS/cm and 26.8 °C, respectively after 60 min of plasma inducement in a time-dependent manner. The mycotoxins decay kinetics after PAW application were illustrated. Mycotoxins degradation efficiency significantly increased (P<0.05) with increasing water activation time. A significant increase (P<0.05) in AFB1, OTA and FB1 degradation levels was reported mainly during the first 10 min of treatment for AFB1 and the first 15 min for OTA and FB1 to record values of 28.33, 32.14 and 34.62% and 33.80, 40.70 and 43.38% after 60 min of feed exposure to PAW-30 and PAW-60, respectively. Significant differences (P<0.05) between examined mycotoxins in their degradation levels were recorded, where FB1 exhibited the highest degradation levels. Generally, feed compositions were slightly affected by PAW and fats were still having good quality. Conclusion: The possibility of PAW for degrading more than a quarter to a third of the original quantity of targeted mycotoxins in poultry feeds after 10 min of treatment with a slight effect on feed quality.
This study compares the effects of plasma jet and plasma-activated water on teeth root canals contaminated with Escherichia coli bacteria. A plasma jet system was developed for biological purposes that operate at atmospheric pressure. The plasma jet works with argon gas and is generated by a power supply, which supplies a sinusoidal alternating voltage of 12 kV of 20 kHz frequency. The system was optically diagnosed, as it was found that the peaks of the nitrogen spectrum were obtained at the wavelength (300- 450) nm with the appearance of hydroxide peaks at 380 nm. Extracted teeth with one root canal were used, which were contaminated with bacteria and divided into two groups to be treated with a plasma jet and plasma-activated
... Show MoreThe protective effects of red cabbage and garlic extracts against liver, kidney and thyroid gland damage induced by fumonisin B1 (FB1) in male mice were studied. Sixty mice divided in to six groups. Group one are the healthy mice, Group two are mice that received a daily oral dose of only FB-1 (100 µg/kg.b.w) for 1 month, Group three: are mice that received red cabbage extract (500 mg/kg.bw) plus FB1, Group four: are mice that received red cabbage extracts, Group five: are mice that received garlic extract (500mg/kg.bw) plus FB1, group 6:are mice that received only garlic extract. After finishing the experiments, samples of blood were used for biochemical examination. The results indicated
... Show MoreA simple, fast, selective of a new flow injection analysis method coupled with potentiometric detection was used to determine vitamin B1 in pharmaceutical formulations via the prepared new selective membranes. Two electrodes were constructed for the determination of vitamin B1 based on the ion-pair vitamin B1-phosphotungestic acid (B1-PTA) in a poly (vinyl chloride) supported with a plasticized di-butyl phthalate (DBPH) and di-butyl phosphate (DBP). Applications of these ion selective electrodes for the determination of vitamin B1 in the pharmaceutical preparations for batch and flow injection systems were described. The ion selective membrane exhibited a near-Nernstian slope values 56.88 and 58.53 mV / decade, with the linear dy
... Show MoreThe development of a reversed phase high performance liquid chromatography fluorescence method for the determination of the mycotoxins fumonisin B1 and fumonisin B2 by using silica-based monolithic column is described. The samples were first extracted using acetonitrile:water (50:50, v/v) and purified by using a C18 solid phase extraction-based clean-up column. Then, pre-column derivatization for the analyte using ortho-phthaldialdehyde in the presence of 2-mercaptoethanol was carried out. The developed method involved optimization of mobile phase composition using methanol and phosphate buffer, injection volume, temperature and flow rate. The liquid chromatographic separation was performed using a reversed phase Chromolith® RP-18e column
... Show MoreStatistical methods and statistical decisions making were used to arrange and analyze the primary data to get norms which are used with Geographic Information Systems (GIS) and spatial analysis programs to identify the animals production and poultry units in strategic nutrition channels, also the priorities of food insecurity through the local production and import when there is no capacity for production. The poultry production is one of the most important commodities that satisfy human body protein requirements, also the most important criteria to measure the development and prosperity of nations. The poultry fields of Babylon Governorate are located in Abi Ghareg and Al_Kifil centers according to many criteria or factors such as the popu
... Show MoreThis study sought to determine malformation caused by Ochratoxin-A (OTA) on mouse embryos. Twenty adult female white Swiss mice (mus msculus) were divided into four groups, with five females per group, and with one male placed with two females in a cage. Avaginal plug was observed in the early morning and the day of mating was considered as day of pregnancy followed by the first day of pregnancy. Three sub lethal concentrations of OTA were applied to the respective groups (other than the control), 1mg/kg, 2mg/kg and 4mg/kg. The animals were given 0.1 ml per 10 gm body weight per concentration of OTA once a day during days 7-14 of pregnancy. The control group animals were given distilled water. The pregnant mice were dissected, and the embry
... Show MoreGranular carbon can be used after conventional filtration of suspended matter or, as a combination of filtration - adsorption medium. The choice of equipment depends on the severity of the organic removal problem, the availability of existing equipment, and the desired improvement of adsorption condition.
Design calculations on dechlorination by granular - carbon filters considering the effects of flow rate, pH , contact time, head loss and bed expansion in backwashing , particle size, and physical characteristics were considered assuming the absence of bacteria or any organic interface .
The eggshell cuticle is the proteinaceous outermost layer of the eggshell which regulates water exchange and protects against entry of micro-organisms. Outer eggshell and cuticle protein was extracted from domestic chicken. The aim of the research is to find out the effect of the treated and untreated nano particles of egg shells with micro wave cold plasma on the effectiveness of E. coli (negative bacteria) that infect the skin and measure the diameter of bacterial inhibition zone, the eggshell has been prepared by a chemical method (sol gel) and measure the level of acidity and the PH is neutral. The result of Atomic Force Microscope (AFM) shows that the particles diameters become smaller with nano-particles solution than for egg
... Show MoreThe goal of this research is to use optical emission spectroscopy to investigate the parameters of exploding silver wire plasma. The silver discharge plasma's emission spectra were recorded and studied. For silver wire of diameter 0.4 mm and different currents 75,100, and 125A in deionized water, the plasma electron temperature ( ) was calculated by Boltzmann plot and container plasma medium temperature by thermal camera, and the electron density ( ) was computed by Stark broadening using the hydrogen (H line) at 656.279 nm With increasing current from 75 to 125 A, the electron density (ne) increased from 3.160× to 8.762× , while electron temperatures increased from 0.571 to 1.334 eV under the same conditions.
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