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Evaluation of the Antibiofilm Activity of Laurus nobilis Leaves Extract and Assessment of Its Effect on fimA and papC genes in Escherichia coli isolates

Background: The beneficial gut bacterium E. coli can cause blood poisoning, diarrhoea, and other gastrointestinal and systemic disorders. Objective: This study amid to examines the antibiofilm activity of Laurus nobilis leaves extract on E. coli isolates and compares pre- and post-treatment gene expression of fimA and papC genes. Subjects and Methods: Ten isolates of E. coli were obtained from the Genetic Engineering and Biotechnology Institute, University of Baghdad, which was previously collected from Baghdad city hospitals and diagnosed by chemical tests, the diagnosis was confirmed using VITEK-2 System. The preparation of the aqueous and methanolic Laurus nobilis leaves extracts was done by using the maceration method and Soxhlet apparatus respectively. HPLC were conducted to determine the active compounds in the extracts. Moreover, molecular detection of fimA and papC genes and analysis of the gene expression by comparing the isolates treated with sub MIC of methanolic L. nobilis leaves extract with the untreated isolates. Results: Methanolic and aqueous extracts contained alkaloids, tannins, phenols, saponins, flavonoids, and glycosides. Seven polyphenolic compounds, four flavonoids derivatives (Apigenin, Luteolin, Rutin, and kaempferol) and three phenolic acids (Caffeic acid, Gallic acid, and Syringic acid), were identified by matching retention time with the standards. Laurus nobilis methanolic leaf extract inhibited 90% and 100% of E. coli biofilm development at 32 and 64 mg/ml. Conclusion: The result of the gene expression revealed that there is a decrease in the expression of the fimA and papC genes. The present study concluded that the Laurus nobilis leaves extract have rich phytochemical contents, so the methanolic extract had an excellent reduction effect on biofilm formation and showed remarkable down-regulation on the papC and fimA genes, which are responsible for the biofilm formation in E. coli.

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Publication Date
Mon Sep 02 2019
Journal Name
Journal Of Legal Sciences
The Special Conditions for the Crime of Extradition in Accordance With International Law Provisions and Iraqi Law

The title of this research is "The Special Conditions for the Crime of Extradition in Accordance With International Law Provisions and Iraqi Law." The examination of the conditions of extradition is of paramount importance because it establishes the general provisions on the basis of which extradition is made, if such conditions are met at the time of the issuing of the extradition decision. Crimes vary according to their types, gravity and description. For this reason, the conditions for determining the crimes in which extradition is permissible have been established. These conditions are that the crime committed is of a certain gravity. The second condition is dual criminality in the requesting State and the States to be extradited; th

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Publication Date
Mon Nov 30 2020
Journal Name
Journal Of Economics And Administrative Sciences
Building a model to reduce the cost of hajj in the Iraqi Authority for Hajj and Umrah

The research is summarized in the construction of a mathematical model using the most common methods in the science of Operations Research, which are the models of transportation and linear programming to find the best solution to the problem of the high cost of hajj in Iraq, and this is done by reaching the optimum number of pilgrims traveling through both land ports and the number Ideal for passengers traveling through airports by Iraqi Airways, instead of relying on the personal experience of the decision-maker in Hajj and Umrah Authority by identifying the best port for pilgrim's travel, which can tolerate right or wrong, has been based on scientific methods of Operations Research, the researcher built two mathematical models

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Publication Date
Tue Mar 19 2019
Journal Name
Political Sciences Journal
Dialectical relationship between the civil state and the application of Sharia In contemporary Islamic political thought ج

The political movements of Islam are among the most prominent phenomena of the popular uprisings witnessed by the Arab world. However, this rise and the rise of some movements led to many problems on the political theses of Islam, especially those associated with the ideas of Islamic ideologues and their slogan Legitimacy and the authorities as the origin of the divine, and said the application to achieve the Islamic solution, and then became the state in theses of some Islamists a tool to apply the law and then the preservation of religion.

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Publication Date
Sun May 10 2015
Journal Name
Al-academy
Compound Shapes in the work of the artist Max Ernst and Ali Najjar: صاحب جاسم حسن البياتي

the Research problem entitled: (compound shapes in the work of the artist Max Ernst and Ali Najjar) the nature of the concept of compound shapes and mechanisms of functioning in contemporary art done. The research consisted of(4) four parts: the first part is dealing with the methodological framework of the Search represented the research problem , which is manifested through this study to reveal the technical features of the composite shapes, that linked achievement both artists (Max Ernst) and (Ali Al Najjar). Which is crystallized into two objectives as follows: 1 - Detection of the technical features of the composite shapes in the work of Max Ernst and Ali Al-Najjar.2 - Detection of similarities and differences in the recruitment of

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Publication Date
Tue Dec 01 2020
Journal Name
Journal Of Economics And Administrative Sciences
The economic implications of the housing problem In Iraq and ways to address it - a theoretical research

The problem of housing in Iraq is a long-rooted and rooted problem, and it needs a great effort to find out its causes and thus give essential points that contribute to alleviating and addressing it, and it is worth noting that research into the housing problem and the housing sector in Iraq is not done in isolation from other sectors and studies. As well as the economic policies pursued and the social and political conditions. It is known that the Iraqi economy is a unilateral (rentier) economy, that is, it depends almost entirely on exporting oil and obtaining revenues, and that the economic decision and economic policies followed in Iraq are greatly affected by several factors, including the economic, social and political fact

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Publication Date
Tue Apr 01 2008
Journal Name
Journal Of The Faculty Of Medicine Baghdad
the role of hyperlipidemia on nerve conduction

Background:

previous reports suggested a connection between hyperlipidemia and neuropathy

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
On The Dependent Elements of Reveres Bimultipliers

The objective of this paper is to study the dependent elements of a left (right)
reverse bimultipliers on a semiprime ring. A description of dependent elements of
these maps is given. Further, we introduce the concept of double reverse ( , )-
Bimultiplier and look for the relationship between their dependent elements.

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Publication Date
Mon Jul 20 2020
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
On the Space of Primary La-submodules

     Suppose that F is a reciprocal ring which has a unity and suppose that H is an F-module. We topologize La-Prim(H), the set of all primary La-submodules of H , similar to that for FPrim(F), the spectrum of fuzzy primary ideals of F, and examine the characteristics of this topological space. Particularly, we will research the relation between La-Prim(H) and La-Prim(F/ Ann(H)) and get some results.

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Publication Date
Tue Sep 01 2020
Journal Name
Baghdad Science Journal
On The Normality Set of Linear Operators

            In this paper, the Normality set  will be investigated. Then, the study highlights some concepts properties and important results. In addition, it will prove that every operator with normality set has non trivial invariant subspace of  .

 

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Publication Date
Sun May 17 2020
Journal Name
Iraqi Journal Of Science
On the Construction of d-Koszul algebras

An algebra has been constructed from a (D, A)-stacked algebra A, under the conditions that , A 1 and . It is shown that when the construction of algebra B is built from a (D, A)-stacked monomial algebra A then B is a d-Koszul monomial algebra.

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