Background: Raoultella planticola is a developing pathogen that can infect both humans and animals. Raoultella spp. commonly colonize the gastrointestinal and upper respiratory systems. It usually causes pneumonia, bile tract infections, and bacteremia. Aim: In this study, the anticancer activity of an isolated brown pigment of a Raoultella planticola isolate was tested in vitro. Methods: The laboratory examination included culturing in brain-heart infusion broth, conducting biochemical tests, using the automatic VITEK2 Compact for bacterial isolate identification, performing the MTT assay to evaluate the cytotoxic effect on both the mammary cancer cell line (AMN3) and normal rat fibroblast (REF), and utilizing enzyme-linked immunosorbent assay to measure cytokine levels. Results: R. planticola was found in cow nasal swabs with respiratory problems. The extracted pigment had a significant cytotoxic effect (decreased cell viability) at doses of 2067.5 μg/mL (p < 0.0001 and 0.0001, respectively) and 1033.5 μg/mL (p < 0.0001 and 0.003, respectively). However, the lowest dose (516.875 μg/mL, p > 0.43 and p > 0.18) could not inhibit the growth of malignant or healthy cells. The first and second highest pigment concentrations reduce IL-1β and TNF-α and increase Fas in the AMN3 cell supernatant (p < 0.05). In the AMN3 cell supernatant, the lowest concentration significantly increased IL-1β levels (p < 0.0472) and Fas levels (p < 0.0146), but did not affect TNF-α levels (p < 0.1274). Pigment concentrations at the first and second highest levels significantly reduced IL-1β and TNF-α levels in the REF cell supernatant (p < 0.05), whereas the lowest concentration did not. All pigment doses did not affect the Fas levels in the REF cell supernatant (p > 0.05). Conclusion: In a concentration-dependent manner, the extracted pigment may exert cytotoxic effects and impact cytokine secretion levels in cancerous and normal cell types.
The adsorption behavior of Bismarck brown (BB) dye from aqueous solutions onto graphene oxide GO and graphene oxide-g-poly (n-butyl methacrylate-co-methacrylic acid) GO-g-pBCM as adsorbents was investigated. The prepared GO and GO-g-pBCM were characterized by Fourier transform infrared spectroscopy FTIR, which confirmed the compositions of the prepared adsorbents. Adsorption of BB dye onto GO and GO-g-pBCM was explored in a series of batch experiments under various conditions. The data were examined utilizing Langmuir and Freundlich isotherms. The Langmuir isotherm was seen as increasingly reasonable from the experimental information of dye on formulating adsorbents. Kinetic investigations showed that the experimental data were fitted ve
... Show MoreBackground. Vitex negundo is a medicinal plant rich in bioactive phytochemicals, particularly phenolic and flavonoid compounds, which have been associated with antioxidant and anticancer activities. Natural plant-derived compounds have gained increasing attention as potential alternatives for cancer therapy due to their lower toxicity and diverse pharmacological properties. Aim. To evaluate the antioxidant and anticancer activities of aqueous and alcoholic leaf extracts of Vitex negundo through phytochemical analysis, antioxidant assessment, and cytotoxic evaluation against the human breast cancer cell line (MCF-7). Methods. Leaves of Vitex negundo were extracted using aqueous and 80% methanolic solvents by Soxhlet extraction. Total flavono
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This paper presents an intelligent model reference adaptive control (MRAC) utilizing a self-recurrent wavelet neural network (SRWNN) to control nonlinear systems. The proposed SRWNN is an improved version of a previously reported wavelet neural network (WNN). In particular, this improvement was achieved by adopting two modifications to the original WNN structure. These modifications include, firstly, the utilization of a specific initialization phase to improve the convergence to the optimal weight values, and secondly, the inclusion of self-feedback weights to the wavelons of the wavelet layer. Furthermore, an on-line training procedure was proposed to enhance the control per
... Show MoreBackground: The dimensional change of alveolar ridge is normal effect of teeth extraction, with unfavourable functional and esthetical outcomes that might jeopardize implant placement. To reduce the biological effects of the dimensional changes of alveolar ridge, various surgical procedures have been recommended, called alveolar ridge preservation. In the previous decade, autogenously teeth have been proposed as bone graft substitution in fresh extraction socket with successful results, yet with various preparation protocols. Aim: Evaluate the formation of new bone derived from the freshly extracted teeth that are grafted immediately in the post extraction sockets, in comparison with sockets without graft filling, by using CBCT and to compa
... Show MoreMetals are vital cellular components chosen by nature to perform various critical metabolic activities in living beings. Metal complexes are keys in catalysis, material production, photochemistry, and biological systems. Many studies were conducted to determine their role in biological processes. Transition metal complexes are becoming more important as therapeutic compounds due to their role in inorganic chemistry. Recent advances in inorganic chemistry have created transition metal complexes with target organic ligands that may be therapeutic. Researchers interested in medical applications may find this study useful since it highlights the importance of metals and current developments in medicinal chemistry, such as new approaches to crea
... Show MoreIn the current work, aromatic amines and alkyl halides have been converted to the corresponding azides 2a‒d and 4a-d by the reaction with sodium nitrite and sodium azide respectively for amines and sodium azide for halides. Then, dipropargyl ether derivative of D-mannose 8 has been synthesized from diacetone mannose that has been obtained by the treatment of D-mannose (5) with dry acetone in the presence of sulfuric acid. Then, aldol condensation has been used to prepare diol 7 from the mannose diacetonide 6. The reaction of compound 7 with propargyl bromide in alkaline media has been afforded dipropargyl
... Show MoreThis paper presents a new azo dye 3-[2-(1H-indol-2-yl)ethyldiazenyl]quinolin-2-ol] from the reaction of the diazonium salt derived from tryptamine and 2-hydroxyquinoline. Azo dye was used to prepare a series of complexes with the chlorides of Ni(II), Pt(IV), Pd(II), Cd(II), and Zn(II). Compounds were analyzed and characterized using elemental analysis, magnetic measurement, UV-vis, IR, MS, NMR, and conductivity. The findings demonstrated that the ligand acts as ionic in complex form, O-bidentate, supporting the proposed formula. The complexes generally exhibited tetrahedral and octahedral geometries, except the palladium complex, which adopted a square planar geometry. TGA was used to investigate the thermal characteristics of compo
... Show MoreNanocrystalline TiO 2 and CuO doped TiO 2 thin films were successfully deposited on suitably cleaned glass substrate at constant room temperature and different concentrations of CuO (0.05,0.1,0.15,0.2) wt% using pulse laser deposition(PLD) technique at a constant deposition parameter such as : (pulse Nd:YAG laser with λ=1064 nm, constant energy 800 mJ, with repetition rate 6 Hz and No. of pulse (500). The films were annealed at different annealing temperatures 423K and 523 K. The effect of annealing on the morphological and electrical properties was studied. Surface morphology of the thin films has been studied by using atomic force microscopes which showed that the films have good crystalline and homogeneous surface. The Root M
... Show MoreChronic myeloid leukemia (CML) is a myeloproliferative disorders characterized by formation of Philadelphia chromosome. After disease development, several events may associate with the reduction of anti-tumor immunity. The present study was designed to investigate the immunological profile of innate and adaptive immune response in Iraqi patients with CML. Patients were grouped into untreated (UT), treated (T) with chemotherapy, while another apparently healthy individuals were recruited to represent the control (C) group. Methods: ELISA technique was used to estimate serum levels of GM-CSF, IL-1a, IL-8, IL2, INF-?, IL-4, and IL-10 while SRID was used to estimate serum levels of C4, IgM, IgA, and IgG. Results: Regarding to innate immune resp
... Show More40 blood sample were taken from children infected with kala-azar at age less than ten years, who were admitted to El-Eskan child hospital, and central health laboratory in Baghdad. At the same time 15 sample collected from healthy child for comparing. During research estimated immunoglobulins level ( IgG, IgM, IgA) and (IFAT) IFAT test recorded a higher indicate antibodies level witch was synergistic with infected severity, the stander level of antibodies arrange between 1/16, 1/32 and 1/64 that means it graduate from the lowest one 1/16 into the highest one 1/64 within groups. During infectious the IgG ,IgM level recorded the higher level (2012.9 mg/dl) and (154.3 mg/dl) compared to the control group (874.3 mg/dl),
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