Azotization
Spectrophotometric determination
Sulfamethoxazole.
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New, easy, simple, and fast spectral method for estimation of sulfamethoxazole (SMZ)
in pure and pharmaceutical forms. The proposed method is based on the azotization of
the drug compound by sodium nitrite in an acidic medium and then coupling with
2,3dimethyl phenol reagent (DMP) in a basic medium to yield an orange-coloured dye
which shows λmax at 402 nm. Different affection of the optimization reaction has been
completed, following the classical univariate sequence. The concentration of
sulfamethoxazole about (1-15) μg. mL-1 with molar absorptivity of (14943.461) L.mol1
.cm-1
that obeyed Beer’s law. The detection and quantification limits were (0.852,
2.583) μg. mL-1
respectively, while the value of Sandell’s sensitivity (
...
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