The present study was carried out to investigate the therapeutic effect of Nigella sativa seeds oil emulsion as against Eimeria stiedae experimentally infected rabbits. After isolation of local E. stiedae strain from infected gallbladders, a total of 90 local rabbits (6-8 weeks age) and body weighing (750-1000 gm) were used. They were divided into 6 groups as follow: Group: 1 uninfected and untreated (control group), Group: 2 infected (untreated) with 10,000 sporulated oocysts of E. stiedae, Group: 3 uninfected and given 200mg/kg B.W. N. sativa L. oil emulsion, Group: 4 infected with 10,000 sporulated oocysts of E. steidae. and treated with N. sativa L. oil emulsion of 200 mg/kg B.W on day 16, post infection, Group: 5 uninfected and give
... Show MoreThe clinical, microbiologic and pathologic findings of swollen head syndrome in broiler chickens in Iraq are reported in this study. A consistent clinical feature of this syndrome was swelling of the face. The facial subcutaneous tissue, conjunctivae and nictitating membranes were edematous. Microscopically, there was acute or chronic dermatitis and cellulitis characterized by necrosis of the dermis and subcutaneous tissues, with infiltration by heterophils in acute cases and by macrophages and giant célls in chronic cases. Esherichia coli was constatnly isolated from the facial subcutaneous tissue.
A total of 100 animals were examined during the period of beginning of September - 2010 till the end of March 2011 at dept of Microbiology college of Veterinary Medicine Baghdad University Baghdad Iraq. These animals include 50 cow and 50 sheep. Hairs and scales were submitted to direct KOH mount smear and culture on modified Sabouraud's Dextrose agar medium The direct smear was positive in 40 ( 80%) for both cows and sheep while the growth of dermatophyte was positive in 35 ( 70%) and 38 ( 76%) for cows and sheep respectively. Species identification revealed the presence of Trichophyton rubrum ( 19 isolates ) Trichophyton verrucosum ( 10 isolates ) Trichophyton mentagrophytes ( 5 isolates ) and Microsporum canis ( one isolate ) in cow w
... Show MoreIn this study 72 blood samples were collected from healthy individuals. Thirty blood samples were taken from children with age range (12-18) and twenty blood samples from females with age range (12-15) years old 2-4 after two weeks of vaccination with rubella vaccine. Control group included 12 healthy children and 10 healthy females both did not have rubella vaccine through their life. These samples were tested to evaluate the immune-status of individuals after immunization. In this study specific haemagglutination antibodies and IgM antibodies were measured and total Immunoglobulines (IgM, IgG and IgA) also determined. Lymphocyte transformation and ability of phagocyte cell to phagocytosis were measured. The results showed decrease in t
... Show MoreStudy was conducted to isolate and diagnosis of bacterial which sensitive
and resistance for components of Hg and Pb, and the ability of these isolate
for resistance to remove high concentrations of Pb and Hg from components
derived soil extract. Personalize Enterobacter cloacae 2w isolated filename
as isolated from soil extract wetthass to a concentration of 5 mg Pb/L and
0.25 mg Hg/l. It also found that isolation resistance and treatable with
ordinary two Pseudomonas putida1N resisted concentration was 150 mg Pb/L
and 3.5 mg Hg/l. And isolation resistance added massively vaccine Center
6.2 Log cfu/ml of growth density 6.5 Log cfu/ml with a shot in the Center to
focus 90 mg Pb/L and remove 60% of It also has t
In this study a total of 60 samples of lived fishes (common carp) and frozen fishes were collected from 15 local markets in Baghdad city ,for isolation of Aeromonas hydrophila to determine the hemolytic ,cytotoxic activity of the isolates and their antibiotic susceptibility , 65% of our samples were found to be positive for Aeromonas hydrophila isolation 76.6% were in life fish samples and 53.3% in frozen fish 94.87% exhibited α and β hemolysis, 100% of life fish isolates show β hemolysis while frozen fish isolates show 85.7% β hemolysis and 14.3% α hemolysis , 97.43% of isolates show cytotoxic effect on Vero cells the highest frequency occur in the isolates of life fish group 60.50% , all isolates were 100% resistant to pencillin ,
... Show MoreThis study was carried out to determine the changes in oxidant – antioxidant status and some biochemical parameters in pregnant Iraqi female camels in comparison with non pregnant one. To determine this objective (30) blood samples from pregnant female camels and (30) blood samples from non pregnant female camels was taken at last state of pregnancy (number of animals are 30 in each group) , blood samples were put in the test tubes contain anticoagulant (EDTA) , after centrifugation serum samples were taken for measurement of Malondialdehyde (MDA) , albumin , uric acid , total protein , alkaline phosphatase and urea concentrations.
The results revealed significant increase (P < 0.05) in malon dialdehyde concentration in pregnant
This study was carried out in the laboratories of fish for 90 days in 16 glass aquaria (60 cm, 40 cm and 30 cm/70 L) to investigate the effect of some organic acids salts on some growth indices of common carp fish Cyprinus carpio L. A 128 fish were used with an average weight of (40.8±1) gm. and have been distributed on eight treatments with two replicate and eight fish for each replicate. Formulated eight diets that equally iso-nitrogenous (33.85%) and iso-caloric (1535.605 KJ/gm) were investigated. Organic acids salts (Sodium lactate, Sodium acetate and Sodiumformate) were supplemented singly (0.5% of each salts) and mixed to the experimental diets (0.25% of each salts). The statistical results showed
... Show MoreThis study is conducted on the samples of urine obtained from buffaloes. Eighty six urine samples were obtained from local buffaloes of different ages, of both sex in Mosul abattoir. The results showed that urine of infected animals was cloudy, dark yellow to reddish color with putrid odor of low specific gravity. Microscopic examination of urine sediment demonstrated increased number of pus cell, erythrocytes, epithelial cell, presence of casts and crystals/ high power filed. Chemical examination revealed elevated urine pH and protein content. Serum examination displayed increase levels of urea and creatinine compered to normal urine samples. Culture of urine samples confirmed 24 positive sample
... Show MoreThe aim of present work is to induce ovulation in 40 female goats in non-breeding seasons and pregnancy diagnosis using RIA of progesterone serum level and trans abdominal ultrasonography with 3.5 MHz prop. The 40 Iraqi goats were naturally inseminated during estrous phase, using fertile backs, after withdrawal of intravaginal impregnated sponges with 20 mg of cronolone (Fluorogestone Acetate progestagen) kept for eleven days and 400 IU of equine chorionic gonadotrophin inject I/M 24hrs. before sponge withdraw. The results indicated that all does were showed (100%) estrous sign, the estrous time was 24-66 hrs. after sponge withdra
... Show MoreSome enzymes of seminal plasma such as, Glutamic oxaloacetic transaminase, Glutamic Pyruvic transaminase, Acid phosphatase and Alkaline phosphatase are essential for metabolic processes which provide energy for viability, motility and fertility of spermatozoa. These enzymes are used as good indicators of semen quality as they measure the plasma membrane stability of spermatozoa, so the aims of study are to estimate the enzymes level and total protein in seminal plasma of Holstein bulls. In present the available knowledge there is no study in Iraq regarding the effect of using magnetically treated water on enzymes and total protein in seminal plasma of Holstein bulls born in Iraq to improve the Artificial Insemina
... Show MoreThe study aimed to investigate the morphological and histological variations of the aortic arch of local breed rabbits (Oryctylagus conniculus). Nine adult local rabbits were obtained to study the aortic arch (four animals for corrosion cast technique, two injected with colored latex and three for histological study). All the rabbits were euthanized and exsanguinatd, for the cast of the aortic arch they injected by self polymerizing resin via the left ventricle of the heart and the specimens were macerated in (KOH %25) for two weeks and the cast of the aortic arch were prepared. Two main branches were identified from aortic arch (except in one cadaver has three branches). The brachiocephalic tru
... Show MoreThe present study were made for analysis of major chemical and biochemical components of twenty hydatid cysts fluids include two group (10 fertile cysts and 10 sterile cysts) obtained from the lung and liver of 29 infected patients admitted to medicine city hospital for operation during a period of 6 month these components were measured by colorimetric assay kits (Biolabo®, Human). Results revealed marked (P<0.05).differences in the mean values, particularly among enzymes (Glutanicoxaloacetic transaminase, Glutanic pyruvic transaminase and Alkaline phosphatase), between fertile and sterile fluids while Lactate dehydrogenase level was exhibited high activities in fertile and sterile iso
... Show MoreThis study aimed to investigate the immune responses (Cellular and humoral) to Klebsiella pneumoniae and Eimeria tenella by using Delayed Type Hypersensitivity Test (Skin Test) and Tube Agglutination Test in sixteen local breed rabbits. Animals were divided into four equal groups; First group was immunized subcutaneously with 1 ml (1000 µg/ml) of sonicated Eimeria tenella oocysts –SETO-ET), second group was immunized subcutaneously with 1 ml (1000 µg/ml) of sonicated Whole Cells Klebsiella pneumoniae antigen –SKWC-KP), third group was immunized subcutaneously with 1 ml (500 µg/ml of sonicated Eimeria tenella –SETO-ET and 500 µg/ml SKWC-KP).
... Show MoreThe present study investigated the effect of synthetic non-methylated oligonucleotides containing Cytosine-phosphate-guanosine dinucleotides (Cytosine-phosphate-guanosine oligodeoxynucleotide) on caprine mastitis with Heat Killed Cryptococcus neoformans Ag. 20 healthy local breed does were used with weight ranging of 25-30 Kg and free of mastitis by examination via California Mastitis Test and Somatic Cell Count. The does were allotted into four equal groups, the first group (G1) was treated intramammary with 100μg/kg of Cytosine-phosphate-guanosine oligodeoxy nucleotide on fifth day postpartum in the right mammary gland while the left mammary gland served as control and were infused wi
... Show MoreThe aim of the present study is to establish the relationship between gestational age and placentome measurement via real-time ultrasound. This study is conducted in the college of Veterinary Medicine at Diyala University. Seventeen multiparous does were synchronized for estrus and naturally served to obtain pregnancy and subsequently scanned using both transducers. Ultrasonic (U.S) examination was performed transabdominally (TA) with sector-probe (5MHz) and transrectally (TR) with linear-probe (7.5MHz), in 10 day interval done started from day 35-135 post mating. The placentome was observed for the first time on day 35 by using TR ultrasonic examination (7.5MHz) linear transducer, as echogenic d
... Show MoreThis study is designed to diagnosis blood Toxoplasmosis in human (women and men) and sheep (male and female) by using Elisa test; as well histopathological examination of heart, liver and lung of sheep were also done, internal organs were also obtained heart, liver and lung. Women diagnosis was performed by collection 96 samples from aborted women ageing from 15-45 years and divided into three groups; first group 15-25 years involved 50 sample, second group 25-35 years include 37 samples; and the third group 35-45 years included 9 samples. The results of Elisa test showed six positive of IgG, (two for each group) and one positive IgM for the second group, a significant (P<0.05) differen
... Show MoreThe study includes six adult healthy hen turkey. The cloaca has three compartments separated by two circular folds and next to rectum is the coprodeum which the largest and measured (2.5±0.2cm) in length and (2.1±0.1cm) in diameter. The middle and smallest is the urodeum that measured about (1.01±0.2cm) in length and (1.2±1cm.) in diameter, that receives the ureter and left oviduct. Posteriorly, the proctodeum that measured about (1.4±0.1cm) in length and (1.6±1cm.) in diameter. The coprodeum mucosa has short and flat villi. The urodeum mucosa has tall branching pyramidal mucosal villi. The mucosa of proctodeum has lost the villi pattern. The three compartments were lined with mucous secreting columnar cell
... Show MoreNewcastle disease is one of serious pathological problems and causes of vast economic losses during 2011-2016 in Iraq. The disease caused high mortalities in all types of poultry nevertheless of vaccination. In this study all samples were collected from infected flocks with clinical signs of the disease. Inoculation of chicken embryonated eggs was carried out for virus isolation, identification, Haemagglutination and Haemagglutination Inhibition assay. Using Reverse Transcriptase-Polymerase Chain Reaction to confirm the presence of the virus, Intra Cerebral Pathogenicity Index and Mean Death Time were used to confirm all the isolates that were velogenic. The important determinant of Newcastle disease virus pathog
... Show MoreEffect of different concentrations of Nisin, Potassium sorbate and Sodium chloride on the keeping quality of the locally produced soft cheese samples against the Staphylococcus aureus viability was conducted during November 2015/ May 2016. Staphylococcus aureus were isolated from 20(73.33%)out of 30 bovine and ovine locally produced soft cheese samples and their identification were confirmed based on the morphological, rapid biochemical reactions and serological properties. Staphylococcus aureus isolates that obtained by plating on the Chromogenic agar were further tested serologically. The highest significant (P<0.05) prevalence levels of Staph aureus were found in the cow'
... Show MoreThe study was conducted to evaluate antitumor effects of green tea (Camellia sinensis) extracts (aqueous and methanolic) on Rhabdomyosarcoma; cell line and a normal cell line; mouse embryo fibroblast; Chemical detections of green tea extracts revealed that the aqueous and methanolic extracts were positive for flavonoids, alkaloids, phenol and glycosides. The percentage growth inhibition of five plant concentrations (50, 100, 250, 500 and 1000 µg/ml) were assessed in vitro using tumor cell lines Rhabdomyosarcoma and normal cell line mouse embryonic fibroblasts. The results revealed that the five concentrations of the plant extracts showed anti-tumor properties in a concentration-dependent manner
... Show MoreThis study was undertaken to explore the adding effect of manganese chloride (MnCl2), co-enzyme Q10 (Co-Q10) as well as α-lipoic acid to Tris extender on Freeze ability of Holstein bulls’ semen. This study was carried out at the department of artificial insemination at the Directorate of Animal Resource, Ministry of Agriculture in Abu-Ghraib, Baghdad for the duration from October\ 2013 to Jun\ 2014, including three experiments. Seven Holstein bulls of 3.5-4 years old were used in this study. Semen was collected via artificial vagina by one ejaculate/ bull/ week. The assessments were conducted for fresh semen, which was later pooled, equally divided for various treatments within each experiment, usin
... Show More
Cryptosporidiosis is one of the most important zoonosis diseases distributed worldwide causes severe diarrhea in animals and is lethal in quails. This study detected Cryptosporidium spp. in quails in Baghdad province Iraq by flotation and staining methods and compared these results with PCR results from the previous study. This research was carried out from January 2022 to September 2022. Out of 180 quails (Coturnix coturnix) in total were investigated. The overall infection rate of Cryptosporidium spp. in quails was 23.9% (43/180). Young quail had a greater infection rate of 44.2% (19/43) while adult quails had a lower inf |
The aim of this study was to perform a comparison between quercetin and deferoxamine (DFO) on the heart, liver, and duodenum in iron overloaded rabbits. Four groups of adult rabbits (each of seven) used in the current study were treated as follows for 28 days: control (C): were injected intraperitoneally (IP) normal saline every 72 hours + normal saline orally daily. T1, T2, and T3 groups were IP injected with iron dextran 100 mg/kg every 72 hours, T1 was left without treatment, group T2 was administrated with quercetin 350 mg/kg orally daily, and group T3 was injected with DFO 125 mg/kg intramuscularly (IM) daily. At the end of experiment, all animals were sacrificed, and tissue specimens w |
أجريت هذه الدراسة في نينوى على الجاموس المحلي، إذ جمعت البيانات عن 154 جاموسنة فني ليلني مخيل ني فني
المحافظة لدراسة إنياج الحليب اليومي وذلك للمندة من 1/7/2010 ولغاينة 18/7/2010 ولخمسنة أينام ميوالينة يالينوم ا و
والخامس والياسع والثالث عشر واليوم السنابع عشنر منع جسنجير درجنات الحنرارة الونغرى والعظمنى. جنم جحلينر البياننات
باسنيعما يريينة ا نمنوذج الخاني العنام ي General linear model-GLM ضنم البرننام SAS لدراسنة جناتير
تهدف الدراسة الحالية التحري عن تأثير المستخلص المائي لثمار نباا الطبا Tribulus terrestis فاي بعا
المعايير الكيموحياتية وهي الفوسفاتيز الطاعادي والحدياد والفوسافا والداوديوم و البوتاسايوم واسلدوساتيرول و الكورتيكاو
ستيرود في ثلاثين أرنب ا مان الاوكور البالغاة قسام حيواناا التررباة علا ساتة مرااميا المرموعاة اسولا هاي مرموعاة
السيبرة ، و المرموعة الثانية جرع ب 051 ملغم\كغم\يوم من المستخلص المائي للطبا ، والمر
هدفت الدراسة الحالية لتحديد تاثير نبات الحنظل فى بعض الصفات الفسلجية كوزن الجسم ، بعض معايير الدم
ووظيفية الغدة الدرقية لذكور الارانب انجزت تجربتين لستين ذكر ارنب بالغ و قسمت بشكل متساوي وعشوائي الى 03
ارنب في كل تجربة . تحوي كل منها على ثلاث مجاميع معالجة وثلاث مجاميع سيطرة وكل مجموعة ضمت 5 ارانب .
في التجربة الاولى والثانية وبشكل منفصل , اعدت الارانب المعالجة) (T1, T2 and T3 groups وجرعت في التجربة
الاولى بجرعة
Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs, causing
acute hemorrhagic enteritis and myocarditis. In this study different mutant strains of the virus
were characterized by polymerase chain reaction (PCR).The fecal samples from infected dogs
suspected for CPV2 infection were collected in a suitable medium. The viral DNA from fecal
samples was extracted using specific kits, PCR were carried out with five different primer,
pCPV-2ab and pCPV-2b, to distinguish the strain prevalent in field condition. The primer
pCPV-2ab recognized both variant CPV-2a and CPV-2b, whereas the primer pCPV-2b
recognized only the variant CPV-2b, using the third primer pCPV to recognize the residual
base pair
In this study, a sero-survillance on ovine and caprine serum samples from all over Iraq
except the governorates of Iraqi Kurdistan region was conducted. It aimed for the detection of
specific antibodies directed against VP7 protein - the sero-group specific antigen of blue
tongue virus – by using a competitive ELISA technique.The results showed that among a total
of 3277 serum samples, 1436 samples were found to be positive forming 43.82% of total
samples, while 1687 samples were negative with 51.48 % and 154 sample were suspicious
forming 4.69 %. The highest positive serum samples were found in Al Basrah Province
(southern of Iraq) with 60.41% and in Al Anbar Province (Western of Iraq) with 61%, while
the lowes
Salmonella typhimurium and salmonella enteritidis were isolated from infected goat and
prepared an antigens of whole cell sonicated antigen of S.typhimurium
(WCS.Ag.S.typhimurium ),whole cell sonicated antigen of S.enteritidis (WCS.Ag.S.
entertidis) and combination of whole cell sonicated antigen (Salmonella typhimurium and
Salmonella enteritidis) (CWS.Ag) . Their efficacy was evaluated by using tube agglutination
test and enzyme linked immune sorbent assay (ELISA). Twenty rabbits were randomly
divided into four groups; the 1st group was immunized by WCS. Ag - Salmonella enteritidis,
2nd group immunized by (WCS Ags .typhimurium), 3rd group immunized by CWCS.Ag
compound and 4th left as control group which injected