The study was carried out to investigate the effect of cryopreservation in liquid nitrogen (-
196ºC) on sperm quality (concentration, motility, morphology) and in vitro fertilization rate (IVF),
24 hours post insemination in mice. The sperms were obtained from caudal epididymis of 20 mature
male mice and prepared by Direct Activation Technique after cryopreservation followed by
insemination the oocyte of 20 female mice using in vitro fertilization procedure. Results showed
that there were significant (P > 0.05) decrease in sperms concentration, motility and morphology post
cryopreservation. In addition, the results demonstrated that cryopreservation significantly decreased
(P > 0.05) fertilization rate in treated mice (36%) compared to control mice (56%). It is conclusion
that frozen sperm can be used without compromising pregnancy chances.
Effect of Cryopreservation on Sperms Function Parameters and In vitro Fertilization Rate in Mice
Cryopreservation
Sperm
In vitro Fertilization
Male mice