Tannins have been demonstrated to inhibit the growth of several chicken illnesses in vitro.
The complex compositions of tannins make it difficult for microorganisms to develop bacterial resistance. This study aimed to evaluate the effect of condensed tannins (CT) extracts on metabolic profile of Avian Pathogenic Escherichia coli (APEC) using Nuclear Magnetic Resonance (1H-NMR). The experimental groups were divided into three groups:control (no CT added), high in procyanidins (PC-CT) group, and high in prodelphinidins (PDCT) group, with exposure times of 0, 10, and 24 h. APEC was observed to respond to CT extracted from Tilia flowers (high PC-CT) and black locust leaves (high PD-CT). The levels of amino acids including lysine, leuc
Peafowl are a species of bird for which there is limited scientific research, despite the importance of the respiratory system on bird health. This study aimed to provide a detailed anatomical examination and comparative description of the lungs in adult male and female indigenous peafowl (Pavo cristatus) to further our understanding of the respiratory system in this species. Sixteen healthy adult peafowl (8 peacocks and 8 peahens) were obtained from commercial markets in Dyalla, and Baghdad, Iraq. The birds had a mean live weight of 3.5±0.59 kg for females and 3.0±0.39 kg for males. After being anesthetized, th |
E. coli O157:H7, is one of the main causes of diarrhea and the most prevalent bacterial infection that causes serious illnesses. This research was conducted to investigate the effect of Capparis spinosa fruit ultrasonic extract against resistant E. coli O157:H7 was isolated from stools of calves that were suffering from diarrhea; the bacteria were identified by the Vitek 2 system and a latex agglutination test. C. spinosa was extracted by ultrasonic waves water bath. The phytochemicals were carried out on C. spinosa fruit extract to detect the secondary metabolites. The MIC of the ext |
The aim of this study was to investigate the prevalence of Theileria equi infection in racing horses in Baghdad governorate, utilizing clinical signs, microscopic examination, molecular assays, and hematological tests to identify significant differences between infected and non-infected horses. Between January and December 2021, a total of 160 racing horses from three locations in Baghdad governorate (Al-Amiriya Equestrian Club, Arabian horses in Alzwraa Zoo, and Iraqi Equestrian School) were randomly selected for exami |
Bovine rotavirus was successfully isolated in vero cell line and secondary calf kidney cell culture from faecal samples of newly bom calves affected with diarrhoea. The isolated viruses were cultivated from cell culture produced cytopathogenic effect which consisted of
... Show MoreThe carotid body in an endogenous duck was closely related to the ventral surface of the common carotid bifurcation zone caudal to the parathyroid glands. The carotid body was characterized by its compact and fusiform shap
... Show MoreFourteen different bacterial species were used in a study of their abilities to survive on cement and porcelain surfaces. A dose of 5x10 cfu/drop (50 ul<
... Show MoreThe stomach, small and large intestine of 140 donkeys were examined for Gastrophilus larvae. The rate of infection
... Show MoreImmunofluorescence on incoulated cell cultures, latex agglutination test and enzyme-linked immunosorbant assay (ELISA) were compared
... Show More
This study aimed to assess the hepatoprotective efficacy of quercetin against hepatotoxicity induced by cyclophosphamide in a rat model. A total of 28 male Wister albino rats (Rattus norvegicus), with body weights ranging from 195.5 to 198.2 g and approximately three months of age, were randomized into four different groups: the untreated Control group received no interventions; the CYP group was treated with an intraperitoneal injection of cyclophosphamide at a dose of 200 mg/BW; the Qt group received an oral administration of quercetin at 100 mg/kg BW daily for ten days; and the combined (Qt+CYP) group received quercetin orall |
Mycotoxin aflatoxin B1 (AFB1) is a threat to food safety and human health because it is present in animal feed and is metabolized into aflatoxin M1 (AFM1), a more toxic form, during lactation. The aim of this study was to quantify AFM1 concentrations in raw milk of buffalos, cows, sheep, and goats sampled randomly from four regions within Baghdad Province, Iraq, and to compare these levels with the maximum allowable levels set forth by the European Commission (EC), the Iraqi Standard Specification (IQS), and the food and Drug Administration (FDA). The carry-over of AFB1 from feed to AFM1 in milk were also calculated for each of the studied species. A total of 200 random samples, including 50 each from c |
Muco-adhesive gel formulations are advantageous in extending the stay at the nasal absorption place, promoting drug absorption. Frovatriptan succinate (FVT) exhibits a 35% oral bioavailability and undergoes hepatic metabolism, making it a viable candidate for nasal delivery. This study aimed to assess novel FVT intranasal formulation for brain targeting in rat animal models. A total of 78 female rats (Rattus norvegicus domestica, Wister albino rats) were randomly divided into three groups: group A (considered a negative control), group B (includes 36 rats given FVT IV solution), and group C (includes 36 rats given FVT binary ethosome in situ gel intranasally). Drug level |
Acetaminophen (N-acetyl-para-aminophenol, APAP), widely used for pain relief across specie, poses significant toxicity risks. This study aimed to assess the acute toxicity profile of APAP by determining the median toxic dose (TD50) in a murine model. Sixty male Balb/c mice, aged 8 weeks and weighing 20-30 g, were randomized into six equal groups. Five groups received single oral doses of APAP (150, 200, 300, 500, and 700 mg/kg BW), while the control group received distilled water. The TD50 was computed utilizing the probit method. Animals were monitored for 24 h for any sign indicative of clinical toxicity. Post-exposure, liver and kidney necropsies were conduc |
Over one third of the world’s crops– including fruits, vegetables, nuts, spices, and oilseed–require insect pollination, and human reliance on pollination services by honey bees (Apis mellifera) to promote these crops continues to rise due to increasing demands from growing human populations. Identifying the effects of urbanization on genetic diversity on this pollinating insect is important in the field of bioscience. This study aimed to investigate genetic diversity of A. mellifera in Kwara State, Nigeria, using the random amplified polymorphic DNA (RAPD) marker. Thirty honey bees were simultaneously collected from both rural and |
This study concentrate on the effect of synthetic insulin in laboratory animals, 80 white mice, randomly divided into four main groups of males and females control –untreated, treated as low dose, intermediated dose and high dose, then every group also divided in two subgroups, the first subgroup was given Actrapid and second subgroup was given Monotrade , the tested material was given daily subcutaneously untreated control group .
The injection was continued until animals were killed after 24 weeks of treatment .Clinical, macroscopical and microscopical studies of experimental mice in comparison with the control group and the result were as follows :
1- Injected mice showed normal activity, with increase body weight an
... Show MoreThe study was conducted on 107 patients of cutaneous leishmaniasis, from
Dour town, Salahadeen province attending the Dermatology Department of
Saddam General Hospital, Tikrit.
Patients were divided to 4 groups for purpose of treatment and comparison,
followed up for two months duration.
Group A: treated with intralesional hypertonic sodium chloride solution (9 %
HSCS).
Group B: treated with intralesional pentostam injection, 1 cc. per day for 6
consecutive days.
Group C: treated with silver nitrate cauterization one to two doses of three days
interval.
Group D: left without treatment as a control group.
It was found that the efficacy of 9% hypertonic sodium chloride solution and
pentostam were 94%
This study was carried on 24 adult multipara Iraqi ewes in experimental
farm related to College of Veterinary Medicine, University of Baghdad during
2005. This study revealed the effect of prostaglandin F2 during puerperium
period through the evaluation of vaginal epithelial cells by using vaginal smear
methods. Intramuscular injection of 1 ml prostaglandin F2 (Iliren®) (tiaprosttrometamol=
0.196mg) was done in the 4th days after parturition to 12 ewes. The
vaginal smears were taken in the 4th, 16th and 28th days. Histological
examination revealed that the intermediate epithelial cells were a predominant.
Keratinized cells were takes place in all epithelial cells. Vacuolations were
present in the cytopla
This trail was carried out on twelve Awassi ewes in the Animal Farm of College of Veterinary Medicine in June 2005 to study the cellular changes that occur in the vaginal epithelium after parturition, by using vaginal smear method. The post-parturient period was divided into three periods; 4th, 16th and 28th days after parturition. Each period presented different sizes of vaginal epithelial cells, some of them were predominant. Keratinization takes place in all periods. The 2nd period was characterized by cellular division, presence of vacuolated epithelial cells and WBCs mainly neutrophils cells. The conclusion, variations in the vaginal epithelium may relate to the physiological status and the hormonal activity of the animals.
This study was carried out to determined the effect of adding 0,150,200 ppb
of organic chromium in feed on dressing percentages and meat composition of three commercial broiler strains (Lohman ,Ross and Hubbard) .A total of 450
day old chicks (150 chicks per strain)were distributed into nine treatments and each treatment
Divided into two replicates (25 birds per replicate) .The data obtained reveald the following results:
Adding 150 and 300 ppb organic chromium increased significantly ( P<0.05) dressing percentages with or without giblets , and no significant differences appeared in edible organs percentages includes liver, heart and gizzard , also adding 150 or 300 ppb of organic chromium increased mea
... Show MoreSurvey on distribution of hydatidosis in cattle in Anbar province have been done
during period from 1/2002-12/2002 .This survey showing that 8.9 ℅ of cattle
suffering from hydatosis also showing that the infection of cows had been
occurred in all months of the year ,meanwhile the bulls show resistance to the
infection of hydatidosis .There was direct relation between the age of cattle
(male&female)&infection of hydatidosis .About 879 of slaughtered cattle being
inspected in Abattoir of province during the year of 2002
The aim of this study was to diagnose fetal mortality and the time of
occurrence using real-time ultrasonography. 118 ewes were included in this
experiment, to synchronized estrus of 47 ewes were carried out by using
intravaginal sponges containing 40 mg progesterone for 14 days and then
injected intramuscularly with 500IU of eCG at the time of sponge withdrawal,
while 71 ewes were not treated and left to cycling normally. All ewes were
mated naturally. In order to determinate of fetal numbers and observing fetal
mortality, ewes were examined by 5MHz real- time ultrasonography at interval
20-24, 25-29, 30-34, 35-39 and after day 40 after breeding. The results showed
that embryonic mortality rate in non treated
To study the prevalence of brucellosis among buffalo in Baghdad, 144
serum samples were collected and tested serologically using Rose Bengal Plate
Test (RBPT) and Tube Agglutination Test (TAT), also 44 milk samples tested
by Milk Ring Test (MRT). For isolation and identification of Brucella species
and behavior, 15 buffalo aborted fetuses were used. And for evaluation of the
health hazzard caused by the consumption of the locally manufactured cream
from the milk of buffalo, 76 cream samples were used for cultivation.
The results of the RBPT, TAT and MRT revealed the prevalence of the
disease in the percentages (38.19%, 29.86% and 20.45%) respectively.
It was found that Brucella abortus was the main cause of abo
Genetically altered stable non reverting aromatic dependent (aro)
Salmonella typhimurium,strain SL1479 was administrated intramuscularly to
healthy pregnant guinea pigs as alive vaccine. Twenty one pregnant guinea pigs
were divided into two groups, the first group (15 animals) was vaccinated twice
with 1ml containing 17 C.f.U /ml approximately fourth and second week preparturition
and the second group (6 animals) injected with 1ml trypticase soy
broth (TSB) as a control group.
Adverse reaction to vaccination were not observed in the pregnant guinea
pigs, which parturated normally. The vaccine induced humoral and cellular
immune response as measured by tube agglutination test and delayed type
hypersensitivity
A total number of cows artificially inseminated and became pregnant in
Sulaimani region during 1999-2002 were 36952 and 15020 respectively.
The year 2001 showed the highest numbers of those inseminated and became
pregnant,while year 1999 showed the lowest numbers,1039 (5.197% ) .
The overall mean of fertility rate was 40.647% however the total number of
cows those treated with hormone was 8101 ( 21.92%) .
In August 2002 the total number of cows artificially inseminated was
2070, Sharazoor center performed the highest numbers followed Rania center
then Kalar and Chamchamal, while Sulaimani center performed the lowest
numbers. The fertility rate after GnRH treatment was found to be (74.71% )
while the fertilit
The evaluation of gingerol as medical entities in Iraq and Jamaica cultivated
ginger was done in this experiment. Two different types of cultivated ginger
(Iraq and Jamaica) of the same species (Zingiber officinal) were extracted to
achieve the subject of this study. Extraction was performed by two different
stages: the first stage preparative and extraction. Harvested ginger was prepared
for extraction by wash, denotation, drying, grinding, and extracting by acetone
under heating. The second stage is separation by two steps via TLC and HPLC.
The quality and quantity of oleoresin and gingerol in each step of separation
were checked by spots diameter and RF value of spot migration in TLC ginger
compounds and qua
This study was conducted to investigate the hypolycemic effect of the aqueous
extract and some active ingredients of Nigella Sativa seeds in normal rabbits. Twenty
four male rabbits were divided into six equal groups. The first five groups were received
by gavage needle 160,240,320,400 and 480 mg/kg B.W. of the aqueous extract, while
the sixth group which served as control received normal saline solution. After two hours
of administration, the group which received 320 mg showed the lowest blood sugar level
as compared to control and other treated groups. This dose was considered for the next
studies.
Lectin was isolated from Nigella Sativa seeds by 0.2M phosphate buffer containing
0.15M NaCl at pH 7.0, and the
The study was conducted to find out the effect of some feed additives in
reducing the infection rate of broilers with Salmonella typhimurium.
Forty broiler chicks at one week of age were divided into 4 treatments, 10
chicks each as follows:
T1: uninfected control.
T2: Salmonella typhimurium infected + Iraqi Probiotic.
T3: Salmonella typhimurium infected + Poultrygrow 250.
T4: Salmonella typhimurium infected only.
One day after feeding on feed additives, the birds were experimentally
infected with Salmonella typhimurium through the mouth.
Results show that Iraqi probiotic did not decrease the severity of infection
with Salmonella typhimurium in comparison with those of Poultrygrow 250,
shown by the perc
An epidemiological study was done on 2 groups of children infected
with watery diarrhea in some hospitals of Baghdad (group 1: immunocompetent
outpatients, group 2: immunosuppressed inpatients).
Modified cold Ziehl Neelsen stain was used in diagnosis. The whole
oocysts antigen prepared after conduction of experimental infection in 2 calves
by using isolated oocysts from children stool, and this prepared antigen was
used in Indirect Fluorescent Antibody Technique (IFAT).
The percentage of infection in the 2 groups of outpatients and inpatients
were 21.2% and 32% respectively, and there was a difference in percentage of
infection between the 3 groups of children, the highest was 36.4% in group with
leukemia an
A total of 54 broiler chicks were used to study the steady state levels of
Tilmicocin and Enroflxacin in lung tissues and plasma.
Microbiological assay, using Bacillus subtilis (ATCC 9225) or Saricinea leutus
(ATCC 272) as test organisms for Enrofloxacin and Tilmicocin respectively,
was employed to estimate the drugs levels.
The birds were divided equally into two groups at the age of 21 days.
The first group received Enrofloxacin at an estimated dose of 10 mg/kg body
weight in drinking water for three consecutive days, while Tilmicocin was given
in the same way but at a dose of 15 mg/kg body weight .Samples of lung tissues
and plasma were collected from birds of each group at scheduled periods (6, 12,
18, 2
Transfer factor has been prepared from sensitized guinea pigs against heat
killed Mycobacterium bovis antigen and protective efficacy were evaluated in
guinea pigs that gave 1 ml of transfer factor equivalent 5x108 cell / ml of sensitized
and normal cell donor; then challenged with dose 0.01 mg / animal of virulent
Mycobacterium bovis, After 40 days of challenge all animals were sacrificied.
Histologically: - TFs recipient group showed a mono-nuclear cells
infiltration’s (Lymphocytes & Macrophages) with a mild emphysema of lungs, and
liver showed mononuclear cells ,a small necrotic foci in the lymphocytic organs
(spleen & lymph nodes); the kidney showed mononuclear cells in glomeruli and
urinary tubul