Pigeonpox virus was isolated from severe cases of avipoxvirus infection affecting 64 pigeons in Ba'aquba of Diyala governarate. The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immuno-peroxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection.
This research aimed at identifying the relationship of iron-deficiency anemia caused by insufficient dietary intake and the iron-deficiency anemia caused by parasitic worms such as hookworms. Whole blood was drawn from 40 specimens; 20 males and 20 females, normal healthy controls with age ranges 8-50 year. Blood samples were collected from 80 patients with symptoms of anemia, with age range from 10-50 year. After fecal examination, they were divided into two groups: Group one, iron-deficiency anemia with non-parasitic; and group two, iron-deficiency anemia with parasitic. Blood samples were divided into two container, one for the hemoglobin, other for serum ferritin and elements of iron, zinc in tubes without an
... Show MoreThe present study aims at using the biological techniques in a genotoxicity assessment of low doses of radiation in samples of workers in Al-Tuwaitha site due to decommissioning to radioactive contamination as a result of work. This study included 50 male blood samples, aged 35 - 63 years as well as 35 blood samples from non-smokers and non-alcoholic as control group which included 25 male and 10 female aged 25 -57 years. The endpoints used were sister chromatid exchange (SCE) and hypoxanthine guanine phosphoribosyl transferase (HPRT) mutation assay. The sister chromatid exchange in the human lymphocyte for radiation worker was significantly (P<0.05) higher than in the control group. While the results of mutat
... Show MoreThis research aimed at studying the effect of different concentration of hydroalcoholic leaves extract of Datura stramonium concentration against pathogenic Candida albicans isolated from clinical cases of diarrhea in cows and dogs in Baghdad province. Hydroalcoholic extract of the leaves of Datura stramonium were prepared in different concentrations for In vitro and In vivo study against Candida albicans. In vitro test includes the determination of minimum inhibitory concentration 50, 25, 12.5, 6.25, 3.12 and 1.75mg/1ml), and it was found that the minimum inhibitory concentration was 3.12 mg/1ml. Whereas the in vivo test was performed for the determination of sensitivity test of Candida al
... Show MoreThis study conducted to find out the protective role of ethanolic extract of Eruca sativa leaves against the deleterious effect of hydrogen peroxide on some aspect of male reproduction in adult rats. Forty adult male rats were randomly assigned into four equal groups as follows: control group received tap water (untreated); group T1 were received tap water containing 0.5% H2O2; group T2 were received tap water containing 0.5% H2O2 plus administration of 300 mg/kg. B.W. ethanolic extract of Eruca sativa leaves and group T3 administration ethanolic extract of Eruca sativa leaves only at the same dose of group T2.
... Show MoreThe aim of the study is to compare the histological changes that occur in the sensory neurons of dorsal root ganglion at L6 and L7 levels of the spinal nerve in different age groups in rabbits. Fifteen rabbits were divided into three groups of equal number according to their age (weaning, maturation and adult). Dorsal root ganglion of spinal nerve at L6 and L7 levels were removed and examined histologically under light microscope. Comparison were made in diameters of neurons and their numbers in different age. The results showed a significant (P<0.05) decrease in the number of sensory neurons and a significant (P<0.05) increase in their diameters with advancing age. In conclusion, the structures of sensory
... Show MoreThis study is carried out to determine the prevalence of abomasal nematodes of slaughtered sheep in AL-Kut province from the beginning of October 2013 to the March 2014. A total of 450 abomasum from sheep with different ages and sexes were collected and examined for nematodes detection. The results showed that 311 samples of examined sheep abomasal were infected with Haemonchus contortus. Ostertigia spp. and Parabronema skrjabini and the highest level of prevalence was showed in December (100%) and lowest level was recorded in March (43.47%) and the percentage of infected in young animals (92.35%) compared with adults while the females revealed a high prevalence (75.37%) as compared with males (
... Show MoreA research was carried out to detect Brucella antibodies of sheep and goats in Al-Sulaimanya governorate by using modified Rose Bengal test and indirect ELISA. A total of three hundred and eleven (311) whole blood samples (160 sheep and 151 goats) were collected randomly from eight different regions in Al-Sulaimanya governorate from unvaccinated flock with different ages. A total percentages of positive result by using modified Rose Bengal test was 14.46% (20% in sheep and 8.6% in goats), while by using indirect ELISA was 27.6% (35.2% in sheep and 19.2% in goats) with significant (P<0.05) differences. It revealed that rates to modified Rose Bengal test were 14.34% in female and 10.09% in males while t
... Show MoreThe aim of this study is to explain the effect of Ethanolic extract 70% of Metracaria chamomella on some physiological parameters in male rabbits. Twelve adult male rabbits were used in this study and were divided equally into two groups: First group was control (C) and received normal saline for four weeks, the second group (Treated group) was intubated orally with ethanolic extract of Metracaria chamomella in a dose 70 mg/kg B.W. for four weeks. Blood samples were collected by heart puncture from each animal at the end of experiment. Blood sample was divided into a part for hematological study and a part for biochemical analysis. The value of serum urea, and creatinine were re
... Show MoreThe current research aimed at obtaining principal data on the morphological developmental changes that could occur during the post hatching periods in the female genital system of the Indigenous Mallard ducks and to conduct that twenty (immature and mature) ducks were used. Gross findings have revealed that the left ovary in 4 weeks sold ducklings appeared small triangular in shape, located at the dorsal part of coelom in contact caudally with the cranial division of the left kidney and cranially with left lung. In 8 and 12 weeks the left ovary has enlarged showed prominent granular surface. The left oviduct appeared as thin, translucent straighten tube with unapparent regions fixed by a thin doubled folds of oviductal ligame
... Show MoreThe aim of the present work is to determine the hematological parameters in clinically normal local breed goats. The study was conducted on 130 goats (40 males of them 20 bucks and 20 buck kids and 90 females of them 20 doe kids, 20 dry does, 25 pregnant and 25 lactating does) aged 6 months-4 years in AL-Najaf province-Iraq. Blood samples were collected from the jugular vein into EDTA tubes during October 2013 untill February 2014. Results showed that the ranges and means ±SE of blood picture were as follows; packed cell volume (PCV) 26-40% and 31.8±0.32%, Hemoglobin (Hb) 8-14.3g/dL and 10.3±0.13 g/dL, Red blood cell (RBC) 10.6-17×106/μL and 13.3±0.14×106/μL, Mean cell volume (MCV) 1
... Show MoreThis study focuses on diagnosis of Clostridium difficile causing Antibiotic – Associated diarrhea and colitis by Elisa method and to detect of C. difficile Toxin A and B in stool samples by Elisa test. Two hundred forty (240) stool samples were collected from children suffering from antibiotic- associated diarrhea and Colitis cases at ages from after birth to 15 years old from Baghdad hospitals. Samples were taken during the period of first of June 2013 until the end of April 2014. In addition to that 80 samples from healthy children of the same age and sex as a control group. Clostridium difficile Toxin A and B in stool samples were detected. Results of this study in
... Show MoreThis study was designed to investigate the effect of dill (Anethum graveolens) seeds in mitigating the complications of induced diabetes mellitus such as nephropathy of adult female rabbits. Thirty adult female rabbits were randomly assigned to 5 equal groups and treated daily for 45 days as follows: Control group (C) drenched tap water, control group intubated orally with 700 mg/kg B.W ethanolic extract of dill (T1), diabetic group drenched tap water (T2), diabetic group intubated orally700 mg/kg B.W of dill (T3) and diabetic group treated with insulin injection 3 I.U S/C (T4). Blood samples were collected by heart puncture at 0 and 45 days of experiment to assess renal efficiency: Serum creatinine, ser
... Show Moreأجريت دراسة تأثيرالعلاج التمهيدي باستعمال المركن كسيلازين وبجرعة (1.1) ملغم/كغم والمخدرالعام كيتامين بجرعة (2.2) ملغم/كغم والمرخي العضلي المركزي جوايفينيسين بجرعة (50-100) ملغم/كغم مع التخدير العام الانشاقي الايزوفلوران 5% على ستة من الخيول العربية الأصيلة لدراسة تأثيرها على بعض التحاليل الدموية والكيموحيوية حيث أخذت عينات الدم قبل التخدير وبعده بمدد 5 و15 و30 و60 و120 دقيقة وبعد 24 ساعة وأظهرت النتائج أن هنا
... Show Moreصممت الدراسة للتحري عن التأثير الفيزيائي الحياتي للأشعة الكهرومغناطيسية على الجهاز التناسلي الذكري للفئران البيض وعلاقتها بتغير كثافة القدرة بوحدات مايكروواط / سم2، وأظهرت نتائج الدراسة الحالية حصول انخفاض معنوي (P<0.05) في عدد النطف في البربخ وزيادة معنوية في النسب المئوية للنطف الميتة والمشوهة عند الجرع 100 و150 و200 مايكروواط /سم2 عند التعريض لمدة (30) يوما ولمدّة (6) ساعات يوميا
... Show Moreيهدف البحث إلى إيجاد التأثير الفيزيائي الحياتي للأشعة الكهرومغناطيسية على بعض معاملات الدم مثل حجم الكريات المرصوصة والتعداد الكلي والتفريقي لخلايا الدم البيض وعلاقتها بتغير كثافة القدرة بوحدات مايكروواط/سم2. حيث استُعمل أربع مجاميع من إناث الفئران البيض (5/ مجموعة). المجموعة الأولى عدت مجموعة السيطرة، المجموعة الثانية عرضت لـ 100مايكروواط /سم2، المجموعة الثالثة عرضت لـ 150 ما
... Show Moreفُحصت 100 عينة براز لبط بشكل عشوائي من خمسة مناطق مختلفة في محافظة بابل لكلا الجنسين و بأعمار مابين ستة شهور إلى سنة لتشخيص طفيلي الأبواغ الخبيئة (Cryptosporidium) وقد أظهرت النتائج إن نسبة الإصابة الكلية 8%. استعملت ثلاثة تقنيات تشخيصية مختلفة في هذه الدراسة لتشخيص أكياس بيض الطفيلي وهي الصبغة الصامدة للحامض المحورة (Modified Ziehl Neelsen) وتقنية التصبيغ بالأيودين وتقنية التطويف بالمحلول السكري المشبع. قيس
... Show Moreهدف الدراسة هو نتاج مركز بروتيني محور من الألياف العضلية لأسماك الجري Silurus triostegus ودراسة تركيبه الكيميائي وخواصه الوظيفية والحسية عند خزنه لمدة 35 يوماً في درجة حرارة المختبر 25-30 °م، واستعماله في الانظمة الغذائية إذ كانت النسبة المئوية للحاصل 18.6%، أما المحتوى الكيميائي للمركزالمحور المحضر فقد لوحظ أنّه احتوى على نسبة عالية من البروتين ومنخفضة من الرطوبة والرماد والدهن ولطيلة
... Show Moreأجريت هذه الدراسة لمعرفة تأثير التغذية الملحية في بعض الصفات الفسلجية لأسماك الكارب الشائع (Cyprinus carpio). وقد استُعمل ملح الطعام في تركيب علائق غذائية بالنسب الآتية 1 و3 و5 % فضلاً عن عليقة السيطرة الخالية من الملح. مثلت كل عليقة غذائية معاملة بنفسها إشتملت على ثلاثة مكررات. استعمل 200 سمكة من الكارب الشائع ووزعت على 12 حوضاً زجاجيا بواقع 6 أسماك / حوض بمعدل وزن30 ± 10 غم. درست تأثيرات التغذية الملحية في ال
... Show Moreأجريت هذه الدراسة بهدف بيان تأثير إضافة بعض مضادات الأكسدة الانزيمية (الكاتليز) والكلوتاثيون المختزل وخليطهما إلى مخفف Trisفي قابلية التجميد لثيران الهولشتاين بعد الحفظ بالتجميد لمدد مختلفة. استعمل في هذه الدراسة سبعة ثيران هولشتاين بأعمار تتراوح بين 2.5–3 سنة. جمع السائل المنوي بوساطة المهبل الاصطناعي بواقع قذفة واحدة/ثور/اسبوع. جُمع السائل المنوي الطازج للثيران جميعها (Pooled semen) وقُسمَ بالتس
... Show Moreأجريت الدراسة في مختبر امراض الأسماك/كلية الطب البيطري جامعة بغداد للمدة بين 1/3 ولغاية 1/6/2103 لمعرفة مدى تاثير سمية مبيد كلايفوسيت اكوا في عدد كريات الدم الحمر وخلايا الدم البيض وتركيز الهيموغلوبين ونسبة عدد الخلايا المرصوصة، فضلا عن تحديد عدد الأنوية الصغيرة في كريات الدم الحمر في الأسماك التجريبية في سمك البني Barbus sharpeyi ، تراوحت أوزانها بين 10-15غم، قيست الجرعة النصف قاتلة LC50، كما ا
... Show Moreأجريت هذه الدراسة لمعرفة مدى صلاحية ثلاثة أنواع من الأسماك المجمدة المستوردة للعراق هي الأسماك الكاملة (التراوت القزحي) Oncorhynchus mykiss علامة Mersin وأسماك الجري من دون رأس Pangasius hypophthalmus علامة الصياد وشرائح سمك الجري) النوع نفسه (علامة الفاخر والمشتراة من ثلاث مناطق في بغداد وهي العامرية والأعظمية والبياع أجريت الفحوص الكيميائية والنوعية لعينات الأسماك المجمدة إذ ق
... Show Moreتناولت هذه الدراسة معرفة التأثيرات السمية لمادة حليب التين في الفئران حيث استخرجت الجرعة القاتلة الوسطية بعد حقنها داخل الخلب والتي كانت مساوية إلى 30 ملغرام/ كغم من وزن الجسم، ثم عملت 4 تخافيف من مادة حليب التين وهي 1 و3 و4 و5 مليغرام/ كغم من وزن الجسم واختبرت على أربع مجاميع من الفئران بالحقن داخل الخلب وبجرعة مقدارها 0.2 مليليتر يومياً لمدة ثلاثين يوماً، بعدها أجريت الصفة التشريحية لحيوانات ال
... Show MoreInfection with Toxoplasma gondii is a cause of fetal death since T. gondii can be transmitted to the fetus through the placenta (transplacental) from an infected mother or at vaginal delivery. Blood obtained from women and sheep to confirm their infection with toxoplasmosis by using Enzyme Linked Immunosorbant Assay test (ELISA) to ditective positive specific anti-Toxoplasma (IgM, IgG and IgM, or IgG) antibodies. This study used two methodes to extract DNA (the first one was a standard extraction commercial method (CM-PCR) of genomic DNA using a commercial kit (Promega, USA), and the second one was the direct heat DNA extraction using microwave oven (MW-PCR) for whole blood sam
... Show Moreهدفت هذه الدراسـة إلى معرفة تأثيـر مستخلصات عشبتي الشيح (Artemisia herba alba) والقريص ((Urtica dioica على نسبة تبوغ طفيلي ال Eimeria tenella في المختبر فقد انخفضت نسبة التبويغ لأكياس بيض الطفيلي في المجاميع المعاملة بالمستخلصات الكحولية والمائية لعشبة الشيح بالتراكيز %0.1 و%0.5 و %1لتبلغ %60 و65 % و%68 و%64 و 70% و75 % على التوالي، كذلك أثرت المستخلصات الكحولية والمائية لعشبة القريص في خفض نسبة التبوغ لتصل ال
... Show Moreإنَ الغاية من هذه الدراسة هو التعرف على ما يحدثه جنس المولود من أثر في حدوث عسر الولادة في أبقار الحليب الأباكير. أجريت هذه الدراسة في العيادة الطبية البيطرية التخصصية بالأمراض التناسلية والتلقيح الاصطناعي في قضاء بلد بمحافظة صلاح الدين، في الحقبة الممتدة من 1/6/2011 إلى 1/6/2014. شملت الدراسة: 90 بقرة بكر كانت تعاني من عسر ولادة. 60 بقرة ذات ولادات ذكور وإن نسبة عسر الولادة (66.67 %)، 30 بقرة ذات
... Show Moreأجريت هذه الدراسة لمعرفة تأثير إضافة مستويات مختلفة من مسحوق خليط الثوم وعرق السوس المضافةإلى العليقة في الصفات الإنتاجية لفروج اللحم. وإستعمل في التجربة 180 فرخاً من سلالة (Ross-308) بعمريوم واحد، وزعت عشوائيا على أربعة معاملات تجريبية بواقع (3) مكررات لكل معاملة (15 فرخ / مكرر). وكانت المعاملات: الأولى المقارنة خالية من الأضافات، الثانية والثالثة والرابعة تضمنت ( 0.25 و0.50 و 1) % من مسحوق خليط الثوم وعرق ال
... Show More The aim of present study to investigate the prevalence of Cryptosporidium Spp. in broiler
chickens in some regions of Karbala province and study the effect of months, sex and age on the
prevalence of parasite. The results were indicated that the overall percentage of infection was
20.71%. The highest rate of infection in Al-Husseiniya which amounted to 22.41% and the lowest
in the Ain Al-Tamur region (18.86%), while the highest rate of infection was in March which
amounted to 30% and the least in May month reaching 11.42%, and by age groups was the highest
rate of infection at the age of five weeks (28.72%) and the lowest at the age of two weeks (13.18%),
and significant difference was recor
The present study was carried out for the identification and molecular characterization of Salmonella spp. isolated from cattle at abattoir by biochemical, serotyping and virulence gene based polymerase chain reaction (PCR) techniques. Eleven Salmonella were isolated from cattle at abattoir, these isolates were cultured and biochemically characterized by double checking with a conventional method and by KB011 Hi Salmonella TM identification kit then confirmed by serotyping and testing for detection of the invA virulence gene by PCR by using a Salmonella-specific 506 bp invA gene amplicon. The biochemical and serotyping results revealed that the 1
... Show MoreThe study was designed to investigate the histomorphology of Miebomian gland in local Iraqi breed goats (coprus hircus).The research work included specimens of upper and lower eyelids of the eyes of 10 head of local breed black Iraqi goats aged 10-14 months (5 male and 5 female) and the routine histological technique was done from fixation till staining of histological sections. The study revealed that the Miebomian glands present in the sub palpebral conjnctiva arranged in parallel position along the upper and lower eyelids of the eye each gland opened besides the hair follicle (cilia) and its secretion are oily (lipid) called miebum. This material very important to spreading the tear on the cornea to p
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