The aims of this study to evaluate the antibacterial potential of standard strains (Lactobacillus acidophilus ROO52 and Lactobacillus bulgaricus LB-12) that produce bacteriocins against E.coli O157:H7 in raw milk. Thirty raw milk samples randomly were collected weekly different markets of Baghdad city in order to investigate the prevalence of E.coli O157:H7 in milk. The prevalence of E.coli O157:H7 in raw milk samples was 6 out 30 (20%) by using the modern chromogenic media with serological latex agglutination test kit. The average diameter of the inhibition zone of crude bacteriocin against E .coli O
... Show MoreThis study was conducted on sixty seven cows of different breeds and ages (non-descriptive cows). The female genital organs were examined carefully through the rectum before slaughter. Blood samples were also collected prior to slaughter to estimate the levels of progesterone and estrogen level. Gross examination of the same genital organs was approved post slaughter. A comparison has been done to assess the accuracy of rectal palpation for diagnosis of some normal and abnormal conditions. The incidence of findings in rectal palpation were 39 (58.2%) normal non-pregnant, 16 (23.88%) normal pregnant and 12 (17.91%) abnormal conditions. While the findings in gross examination were 32 (47.76%) , 18 (26.86%) and 17 (25.37%), respectively , g
... Show MoreThis study was conducted to evaluate the effect of feed restricted (off-feeding intervals) on feed intake, water intake, body weight gain, growth rate, feed conversion ratio, mortality, and dressing percentage of broilers. Two hundred fourty, one day old unsexed, (Ross 308) broiler chicks were randomly distributed into four treatment groups. Each group included three replicates each one had 20 birds. Those assigned as; Control group (T1): birds were fed ad libtium, T2 group feed removed from 8 Am. to 4 Pm., T3 group feed removed from 4 Pm. to 8 Am., T4 group feed removed 8 Am. to 8Am. (skip-a day) all birds were allowed to access feed for above intervals for 8-21 days of age, and re-full fed for the remaining of experiment period 42 days
... Show MoreColonies of E.coli O157:H7 were isolated from 35 raw milk sample and their identification were confirmed based on biochemical reactions and both cultural and serological characteristics. Presumptive E.coli O157:H7 isolates obtained by selective plating on both CT-SMAC and Chromogenic agars were further tested serologically for the presence of both O157 and H7 antigenes using the commercial available latex agglutination test kit. The unhygienic practices in the production of milk in Al-Thahab Al- Abiedh , Abu-Graib, Al-Zedan and Khan Dharie were reflected on the highest significant (p<0.01)prevalence level of contamination with E.coli O157:H7 that appeared to be 80%, 80%, 60% and60% respectively. Homogenization pressure of 1000 psi and
... Show MoreThe objectives of this research were to determine the serum cortisol profiles and their temporal interrelationship during progesterone treatment, induced estrous, laproscopical insemination and pregnancy (antepartum) period in goat, with histological fetal adrenal cortex development.Thefirst part of the experiment included studying Hundred and sixty goat fetuses of different Crown Rump Length (CRL) were collected from slaughter house. The adrenal glands in the goat fetus displayed signs of cytological differentiation around the second half of intrauterine life, 20cm/CRL about 90 days old until full term. From histological observation and physical measurements the present study recorded that the adrenal cortex grow faster from 25 cm/CRL(1
... Show MoreThe objective of the current study was to determine the anatomical features of the tympanic cavity in cattle and buffalo and to be recognize all parts of the tympanic cavity for its clinical purposes. There are general anatomical explanation about the middle ear in anatomy text book, however there are no studies in the literature on the morphology of the tympanic cavity in the buffalo, the region is important clinically as a frequent point of attachment for prostheses. As a recent studies have focused on the reconstruction of defects occurring in these tympanic cavity to aid in the development of new surgical technique. Twelve temporal bones from six heads of adult cattle and buffalo were used, the cavity had been investigated on both si
... Show MoreThis study aimed to evaluate the efficacy of different hormonal treatments protocols (PGF2α ,GnRH, estradiol and progesterone) hormones on reproductive performance of postpartum anoestrus native dairy River buffaloes (Bubalus bubalis), endemic south of Baghdad under field conditions . Present study was conducted on 128 animals that had postpartum anoestrus (PPA) for a period between 4 to 8 months. The animals were subjected to two experiments according to the type of anoestrus. In the first experiment 94 animals (73.5%) with persistent corpus luteum on their ovaries without any signs of estrous (sub-oestrus) were classified into two sub-groups.Sub-group1 (n=47) treated with PGF2α hormone alone and sub-group 2 (n= 47) were treated by tw
... Show MoreThis experiment was aimed to investigate the role of pomegranate seed oil (PSO) in ameliorating the deleterious effects of methionine overload on some histopathological structure of heart and aorta in adult female rabbits. Thirty-Two female rabbits divided into four groups eight animals each, and treated for 42 days daily as follows: the first groups were drenched drinking corn oil, serving as control (group C), second group (group T1) were intubated orally with methionine 100mg/kg. B.W, while the third group (groupT2) were intubated orally with methionine 100mg/kg. B.W and pomegranate seed oil (PSO) 30 mg /Kg. B.W, and the animals in group T3 were intubated orally with pomegranate seed oil 30 mg /Kg. B.W. At the end of the experiment ra
... Show MoreThe aim of the present study is to prepare ethanol extract of Pomegranate peel and the effects of this extract on testicular weight to body weight ratio, Serum cholesterol, testosterone concentration and histopathological changes of testes in rabbits treated with carbon tetrachloride (CCl4). Twenty four adult male rabbits were used. They were divided randomly into four equal groups. Animals were treated for 56 days as following: Rabbits of the 1st group were received 1 ml distal water orally once a day and olive oil 0.5 ml /kg B.W. I.P twice a week as control group. The second group were treated I.P with 500mg / kg B.W. of CCl4 mixing with equal volume of olive oil (0.5 ml/kg B.W.) twice a week (group T1). The third group was received po
... Show MoreThe aim of this study was to compare anatomical and histological of the kidney in three type of birds. The study revealed that, in harrier species the cranial lobe of kidney was the largest with elongated shape. In mallard the cranial lobe was small round-oval, while middle and caudal lobe were lobulated and larger .In chicken the caudal lobe was the largest. Statistically, in harrier the mean length of cranial lobe (20±0.1mm) and the width of the middle lobe (5±0.5mm) were significantly higher than those of mallard (10±0.3mm) (8.0.2mm) and chicken (15±0.2mm) (4±0.4mm) respectively. The mean weight of the kidney in harrier is (5.8±0.20g) this value significantly was higher than those of mallared (8.9±0.11g) and chicken (6.8±0.10g
... Show MoreTo study the bacterial contamination of table eggs in Babylon city, a total of 214 eggs collected from different sources, including 100 from farms and 114 from supermarkets, all samples were cultured for the bacteria on Salmonella Shigella Agar(SS Agar) and nutrient agar. The results of the farms samples showed that there are no growth of bacteria in all samples under study whereas the results of supermarkets samples showed that about 21.05% of supermarkets eggs were contaminated with bacterial strains, and the results showed the presence of Stahylococcus aureus in 10.52% of the samples, Pseudomonas aeruginosa in 7.89%, a Proteus mirabilis in 3.50%, S. epidermidis in 0.87% and Bacillus subtilis in 0.87%. Also, the antibiotic sensitivity
... Show MoreThis study was conducted to isolate and identify the fungi that infect burn wounds in humans and
acquired wounds or wounds resulted from surgical operation in farm animals. A total of 110 cotton
swabs, 60 cotton swabs were collected from wounds to 6 kinds of farm animals, which include 10
swabs for each type of these animals (cattle, sheep, goats, cats, dogs, donkeys). As well as 50 swabs
were collected from human burns. All these samples were cultured onto Sabouraud dextrose agar
(oxoid, England) to detect fungi in wounds of humans and animals in Baghdad city.Identification of
Yeasts were done by use RapID™ Yeast Plus System (remel, USA), While the Identification of
moulds were done in the Central Public Hea
In order to investigate the presence of methicillin or multidrug resistant Staphylococcus aureus
in food-chain especially Cows raw milk and white raw soft cheese and its whey, a total of 30 samples
were collected randomly from different markets in Baghdad Province during December 2012 till
February 2013, in which samples were analyzed by a standard isolation protocols of food
microbiology with some modification processing by new, modern and rapid technology tools such as
chromogenic medium Baird-Parker agar, Electronic RapIDTM Staph Plus Code Compendium Panel
System (ERIC®) Dryspot Staphytect Plus and Penicillin Binding Protein (PBP2') Plus assays; as well
as, studying the susceptibility of isolates to differe
The study was carried out to investigate the effect of cryopreservation in liquid nitrogen (-
196ºC) on sperm quality (concentration, motility, morphology) and in vitro fertilization rate (IVF),
24 hours post insemination in mice. The sperms were obtained from caudal epididymis of 20 mature
male mice and prepared by Direct Activation Technique after cryopreservation followed by
insemination the oocyte of 20 female mice using in vitro fertilization procedure. Results showed
that there were significant (P > 0.05) decrease in sperms concentration, motility and morphology post
cryopreservation. In addition, the results demonstrated that cryopreservation significantly decreased
(P > 0.05) fertilization rate in tr
This study has revealed the importance of natural herbal products to control antibiotics resistant
bacteria which are being a threat to human and animal health and focused on antibacterial potential
of aqueous infusion of Dandelion alcoholic cold extract against Pseudomonas aeruginosa. The
experimental studies include (18) healthy mice were randomly divided in to 6 equal groups, all 5th
group injected with P. aerugenosa 106CFU/ ml (0.05 ml) except 6th group which injected with
phosphate buffer saline (control negative). 1st group killed after 48hr of injection, 2nd group treated
with otucalm, 3
rd treated with Dandelion cold alcoholic extract, 4
th group treated with Dandelion
locally and lactobacillus ac
This study was conducted to find out the effect of licorice extract and eCG on the some
reproductive traits in Awassi ram lambs. This study was conducted on State Board for Agriculture
Researches, Animal Researches station. Twenty four Awassi ram lambs at the age of 67-77 days
were randomly divided into four groups according to the lambing date as follow: Group (A) was
injected with eCG 300 I.U /lamb/week. Group (B) was treated with licorice 300 mg/kg B.W/week
orally. Group(C) also was treated licorice 400 mg/kg B.W/week orally. Group (D) were kept as a
control group. Testes measurements were taken at the 12th week of the study period. The level of
testosterone hormone was measured at monthly intervals. Age of
A total of 660 genital tract of adult ewes slaughtered at Baghdad Abattoirs, were examined for
the gross pathological conditions .One hundred thirty three (20.22%) of them showed different type
of abnormalities of the total genital examined, 157 (23.87%) were pregnant and the rest 503 were
non-pregnant ones. Eighteen (11.46%) of the pregnant and 115 (22.86%) of the no pregnant genitalia
showed pathological conditions, Ovarobursal adhesions 51(7.72%) and paraovarian cysts 35(5.31%)
were the most common abnormalities recorded. In conclusion: Such kind of abnormalities observe in
genitalia slaughtered ewe could be considered as the causes of temporary infertility or permanent
sterility, never the less, the percent
This study was conducted to know the effect of ewe age on oocyte quality as well as the relations
between oocyte viability and normal uterine condition. Eighty three (83) reproductive systems of
non-pregnant ewes were collected from Al-shulla abattoir. The Total oocytes were aspirated from
right ovaries reached 61.45% and 38.55% from left ovaries. Immediately after aspiration, the oocytes
were examined by light microscopic and conceded as mature if surrounded completely with cumulus
oopherus. While the stained oocytes by trypan blue were conceded as dead oocytes and excluded.
According to ewes age the oocytes were classified into (3) groups, the first group ranged between 1-
2 years, second group 3-6 years and th
The aim of this study was to detect the effect of toxoplasmosis on some liver enzyme activity
such as bilirubin concentration, Alanine aminotransferase (ALT), Aspartate aminotransferase
(AST), Alkaline Phosphatase (ALP) in serum. Twenty hundred and fifty women were examining
by Enzyme-linked immunosorbent assay (ELISA). One hundred and thirty-four were infected with
toxoplasmosis with different number of abortion (1-4) times were used in this work. All Serum
ALT and AST activities were increased and ALP and total bilirubin level decreased in infected
women below the normal value. The analysis of the plasma enzyme distribution pattern suggests
that liver cell damage occurred during virulent and low virulent inf
This study was conducted to elucidate the morphological changes of the testis of the domestic
rabbit (Oryctolagus cuniculus) during the postnatal life. The animals were divided in to seven age
groups, eight animals for each group. The age of these groups were (1, 15, 30, 60, 90, 120, and 150
days). The mean weights, volume, length, width, and thickness of both left and right testes were
recorded. The positions of testes in all ages were described. The results showed the testes were found
at sub lumber region at one day postnatally, moved and start ingress the inguinal canal at 15 days of
age, and mostly located moving in the inguinal canals and near to the developing scrotum between
30 and 60 days. This become
The aim of the present study was to investigate the analgesic effect of Acepromazine 0.05 mg.
/kg B. w. and Lidocaine 0.5 mg/kg each alone or in combinations on the flank region in sheep after
giving the drugs by lumbosacral injection. Fifteen healthy adult local breed sheep of both sexes
weighting (17.50-30 kg) were used. Sheep were divided into three equal groups. Group (A),
Acepromazine, group (B) Lidocaine, and group (C) Acepromazine and Lidocaine mixture. Data
were collected immediately before administration of drugs (zero time) as control data. Parameters
were included clinical measures: Heart Rate, Respiratory Rate, Rectal Temperature, Scores of
analgesia of the flank, low abdomen, perineum, tail, hind
This study was investigated to recognize the generative capacity of liver that influenced by administration of estradiole benzoate. Twenty four healthy local breed male rabbits weighed 1.5-2 kg were used .They were classified into two equal groups control and treated groups. Animals anaesthetized by intramuscular injection of Ketamine (35mg /kg B.W) and Xylazine (5mg\kg B.W.) In control group partial hepatectomy of liver was done, while in treated animals were subject to a same operation then received an intramuscular injection 0.1ml of estradiol benzoate 0.2% at 3rd and 5th days post operation. At 3rd days microscopic finding showed biopsy were taken at 3rd, 7th and 14 day, post operation to study the histopathological changes in liver
... Show MoreThe main purpose of this study was to convey a more precise explanation of the intrahepatic
pattern of the tributaries of portal and hepatic veins in sheep and goats. Also to give broad
information on the portal and hepatic vein and there topographic relation that may give a useful
base for the hepatic segmentation, aiming to provide a correct base for surgical procedures and liver
transplantation. Twelve livers collected from adult sheep and goats of both sexes. The portal and
hepatic veins were studied by using cross dissection, corrosion cast and radiographic examination.
Subject of this investigation show the portal vein of the sheep is slightly narrower than that of the
goat. Also the pattern of intrahepat
The present study was conducted in two different farms, including (256) foaling mares in : AL-Laith equine herd and equestrian club in AL-Ameria, 4-14 years old foaling mares which are situated in areas around Baghdad and extended one calendar from July 2011 until July 2012 . These mares were examined for pregnancy diagnosis by trans-rectal ultrasonography using a 5 MHz lineararray transducer between Days 10 and 40 after breeding (breeding day = Day 0). Criteria to assess the accuracy of trans-rectal sonography were overall accuracy (correct diagnoses/all diagnoses), sensitivity (correct diagnoses "pregnant"/all pregnant animals), and specificity (correct diagnoses "non-pregnant" /all non-pregnant animals. The sensitivity, specificity an
... Show MoreTo study the influence of whole sonicated Pseudomonas aeruginosa antigens (WSPAgs) on experimental arthritis induced by this bacteria, 15 rabbits were divided into 3 equal groups. The 1st group was inoculated intraarticular with 0.2 ml of p. aeruginosa suspension (2×108 cfu/ml), the 2nd group was immunized with WSP Ags, and inoculated intraarticular as in the 1st group. The 3rd group was served as negative control group. At 30 day post inoculation the immunized (2nd) group showed increase in the cellular (DTH and IFN-γ) and humeral (IgG) immunity and moderate bacterial isolation from joints, blood and internal organs comparing with other groups. The 1st group showed sever symptoms and inflammatory reaction as well as very obvious gross
... Show MoreBrucella spp are important food pathogen those can be infected the human-being during consumption of contaminated milk and milk products from sheep, goats, and cattle with Brucella spp. In this study the polymerase chain reaction (PCR) for direct detection of Brucella spp. from milk of sheep and cattle were employed to amplify 233bp product of highly conserved regions of BCSP31 gene encoding a 31-KDa cell surface protein in B. melitensis and B. abortus. The results showed that the sheep were more frequent for shedding of Brucella spp in their milk, where appeared (6/50 samples) at (12%). Whereas the cattle appeared less frequency for shedding of Brucella in their milk, which showed (2/50 samples) at (4%). It can be concluded that PCR tec
... Show MoreThe aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of ty
... Show MoreThe current study was performed an investigation in seropositive stray cats with Toxoplasma gondii and to correlate the results of pathological lesions with seropositive results of the cats. To achieve these goals, fifty blood samples and specimens from internal organs (liver, spleen, brain, kidney, intestine and lung) of stray cats were collected from different area of Baghdad Province during the period 1.10.2011-1.10.2012. Seropositive ELISA-IgG was demonstrated that 66 % of stray cats while female expressed (75 %) of seropositive ELISA-IgG were higher than male (30% ) and animals with average age 2months showed high percentage of seropositive ELISA-IgG (100%) as compared with age (adult cat) that expressed( 63.82 %) of seropositive. T
... Show MoreObjectives of this study were to evaluate hematological and biochemical parameters in rats ingested toxic doses of CuSO4.5H2O. Thirty six rats divided into 3 equal groups. First group ingested orally distilled water / day for 3 months and considered as control group , second group was drenched 8 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group1 (T1). Third group was drenched 40 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group2 (T2). Blood collection was done by direct cardiac puncture every month for hematological and biochemical examinations. Hematological parameters showed that anemia caused by CuSO4.5H2O toxicity was of microcytic hypochromic type with significant increase of neutrop
... Show Moreتم تصميم التجربة لمعرفة تأثير اضافة مسحوق الزنك في العليقة في الاداء الانتاجي لفروج اللحم المربى خلال اشهر الصيف غير مجنس بعمر يوم واحد. وزعت الافراخ الى خمسة معاملات Ross فرخا نوع 131 في ظل الاجهاد الحراري . تم استخدام فرخ لكل معاملة تم اعطاء مسحوق الزنك في 32 افراخ وبمجموع 8 كل مكرر من ، وبواقع ثلاث مكررات للمعاملة الواحدة ملغم / 21 ملغم(المعاملة الاولى )، 1 يوم بمستويات 23 العليقة اعتبارا من بداية الاسبوع الثاني وحت
... Show More