This study was conducted by evaluate some haematological and biochemical changes caused by F. gigantica. The haematological and
... Show MoreThe mixing of ginger Zingiber officinale and garlic Allium sativum used at different concentrations ( ginger 10g/kg; garlic 10g/kg; ginger 5g/kg with garlic 5g/kg; ginger 7.5g/kg and garlic 2.5g/kg and ginger 2. 5g/kg and garlic 7.5g/kg in treated 1, 2, 3, 4 and 5 respectively) as a dietary supplements based on their performance in enhancing the fish healthy while control diet was free from ginger and garlic. Haematological parameters of 96 fishes of common carp Cyprinus carpio L. were determined after two months, Red blood cells count (RBCs), white blood cells count (WBCs), hemoglobin concentration (Hb) , and Packed cell volume (PCV) , total plasma protein , albumin and Globulin, liver enzymes (ALT,AST and ALP) using several laboratory
... Show MoreThis study was conducted on 200 samples of females Karadi sheep genitalia contains 207 fetuses . These samples were collected from slaughter house in Sulaimani reigion during the period from June 2007 to the end of May 2008. Sheep genitalia consisted of 193(96.5%) single and 7(3.5%) twin fetuses, amonge them 122 (58.92%)and 84(41.06) were in the right and left horns ,respectively. The genitalia that contain fetuses with clear external sex organs was 141, of these 74 male and 67 female fetuses different Crown Rump Length (CRL), while 66 fetus their sex could diagnosis after abdominal opened. The results showed the weight of the fetal body and testes as well as the length of scrotum were increased with an increasing of the fetal CRL. The a
... Show MoreTwenty two ( 11 from lung and 11 from liver of lung-liver cross infection ) hydatid cysts fluid collected from sheep slaughter in Duhok abattoir and there biochemical analysis done for several parameters (total protein , Glucose, Cholesterol , triglyceride, creatinine , Urea , Uric acid , Calcium and Magnesium ions ) by colorimetric assay kits for the first time in this area . The biochemical parameters varies in there measures as for protein increased significantly ( P<0.01) in lung cysts fluid ,while in liver some increased significantly like cholesterol ( P<0.01) ,triglyceride ( P<0.05 ) , creatinine (P<0.01) , calcium (P <0.01) and magnesium ( P<0.05). In compare with previous studies the results reflected some diff
... Show MoreThis study was conducted on 53 samples of female local Kurdi goats uterus contained one or two fetuses .These samples were collected from Sulaimani slaughter house, A total of 53genitlia were consisted of 36 (67.92%)single and 17(32.08%)twin fetuses ,with a total of 70 fetus,of them 33(47.14%) and 37(52. 86%) were located in the right and left horns, respectively. The number of fetuses with macroscopically clear external sex organ was 55, the total number of male fetuses was 34(48.57%) while the female was 21(30%).The fetuses whose external organ could not be diagnosed were 15(21.42%). The weight of the fetal testes and the length of scrotum were increased steadily with an increasing in the fetal Crown Rump Length(CRL). When the CRL is 2
... Show MoreNerium oleander leaves ethanolic extract at median lethal dose of 520 mg/kg body weight were administered orally to six male mice .Clinical signs of toxicosis in mice began to appear about 1 hr after receiving the oleander extract which included abdominal pain, frequent urination, diarrhea, depression, weakness and convulsive movement and death at the end stage.Four mice died within 4 to 24 hrs and two survived. Haemorrhage spots of varying degrees were observed in internal organs at necropsy.Histopathology revealed extensive tubular necrosis in kidneys with haemosiderin pigment in the cytoplasm of convoluted tubular cells, different degrees of coagulative necrosis in cardiac muscle cells associated with haemorrhage and infiltration of m
... Show MoreThis study was carried out on the pituitary gland of eight male buffaloes 3-5 years old, examined under light microscope. The histological results revealed that the pars tuberalis contained four types of cells, not similar to any other cell types of the pars distalis. This finding and the location of the pars tuberalis at the gate of pars distalis which control the blood circulation give the pars tuberalis a special functional importance.
Safe and effective anaesthetic regimens have been described for use in rabbits,
partially because of the susceptibility of this species to fatal respiratory arrest. The
present study was conducted to evaluate the efficacy of anesthetics, analgesics
combinations an anesthesia in twenty five local breed rabbits. Rabbits were injected
intramuscularly by: Propionylpromazine 0.5mg/kg B.W. as premedictation, after
10minutes later Xylazine and Ketamine Hydrochloride at a dose of 20mg/kg,
50mg/kg respectively. The results of the physiological parameters of the control
group at the period of zero time concerning rectal body temperature, respiratory rate,
heart rate were 38.00±0.29 ºC; 96.36 ±3.33bpm; and 147.20±6.46/mi
Analysis of the decayed apples (van. Starking delicious), collected from the local
market, for patulin mycotoxin, using column chromotography and TLC Plates showed
that the presence of the amount of patulin mycotoxin in the mouldy tissues (1.3 , 2.5 ,
3.4 , 4.2, 5.5, and 6.4 ) cm in diameters were (5.1, 5.8, 6.3, 10.5, 12.3, and 14 ) μg
patulin/g moldy tissues respectively. According to this research also, Patulin
mycotoxin could diffused to the surrounding mouldy tissues. It is found that (0.5and
0.2), (1.0 and 0.5), and (1.5 and 0.5) μg patulin/g tissues in the first and second
centimeters after mouldy tissues 4.2, 5.5 and 6.4 cm respectively, this phenomenon
due to the presence of intracellular spaces in the
The increasing rate of resistance development for commonly used antibiotics have led to search for newer, more effective, affordable and easily available medicine. Medicinal plants have revived as a consequence of current problems associated with the use of antibiotics. Aqueous extracts of nine plants (pomegranate, sumac, sage, anise, hand bull tongue, thyme, cloves, lemon and mint) were qualitatively and quantitatively examined against twenty microbial isolates, mostly food borne including pathogens. (E.coli, Salmonella typhi and Candida albicans) antimicrobial screening was done by agar diffusion (well diffusion) and minimal inhibitory concentration (MIC) methods. Among the screened plants, cloves were the most inhibitor against isolat
... Show MoreThe study revealed that the ampullae of ductus deferens(vas deferens), seminal vesicle and bulbourethral glands are paired in number and well developed in ram and buck except the prostate gland have only disseminated part surrounding the pelvic urethra (embedded in pelvic urethral wall) without body of gland. The anatomical parameters of the ampullae in ram are generally with mean length 4.98± 0.28cm,mean width 0.63 ± 0.04cm ,mean weight 2.24 ± 0.007gms and mean volume 1.4
±0.03cm3.The seminal vesicle have mean length 3.68± 0.05cm, mean width 2.50±0.06cm ,mean weight 4.42± 0.15gms , mean volume 3.35±0.05cm3 .The bulbourethral gland have mean length 1.59± 0.36cm ,mean width 1.493±0.02cm ,mean weight 3.46±0.01gms ,mean volume
In the present study the biological activities of Nigella sativa on limitation and ameliorative detoxification of aluminum were investigated through evaluation of some hematological, biochemical parameters and histopathology. Eighteen mature rabbits were divided equally into three groups. Group 1 was served as a control group; group 2 was administrated orally with distill water both of AlCl3 ( sub lethal dose 200 mg / kg body weight ) and NS in dose of 10 g / liter; group 3 administrated NS only in same dose. The results are revealed significant elevation ( P<0.05 ) in RBCs count, Hb and PCV values in group 3 in comparison with groups 1 & 2; while the WBCs count decreased significantly in group 2 and increased significantly in gro
... Show MoreAqueous extract of DI leaves was given to mice , at graded dose (100&200mg/kgB.W,P.O) to evaluat its antidiarrhoeal potential by using four experimentally induced diarrhoea treated groups each consis of five mice and one control group.Castor oil and magnesium sulphate were used to induce diarrhoea in mice,The extract at both doses showed a remarkable antidiarrhoeal activity manifested by significant delay in onset of diarrhea , decrease in number of wet stools , total number of stools and total weight of fecal output in 4 hours in both castor oil and magnesium sulphate induced diarrheal groups of mice. The results of this study reveal that The aqueous extract of Datura innoxia leaves contains pharmacologically active substance with a
... Show MoreThe aim of this study was concerned on the prevalence of Listeria spp.in the ice cream samples collected from the different pastry shops and downtown stores of Baghdad province during December 2009 till April 2010. A total of 50 ice cream samples (25 locally produced samples from raw and imported powdered milk and 25 imported samples from different origins) were analyzed and processed according to the standard protocols of Food and Drug Administration (FDA) and International Organization for Standardization (ISO) with some modifications. Growth of Listeria spp. was determined in 7 (14%) of total samples as 4 (16%) isolates from locally produced ice creams , from which one isolate was confirmed as Listeria monocytogenes, and 3 (12%) isola
... Show MoreThis study was designed to evaluate the anticancer, effects of the ethanolic (EE), cold aqueous (CAE), and hot aqueous (HAE) extracts of Sonchus oleraceus on cancer cell lines (in vitro). In vitro study was performed on three cancer cell lines (murine mammary adenocarcinoma AMN-3 cell line, laryngeal carcinoma Hep-2 cell line) and rat embryogenic fibroblast (REF) as normal cell line. Periods of exposure of cell lines were measured at 24, 48, and 72-hr in a microtitration plate under complete sterile conditions. Different concentrations starting from (78.125 to 10000) μg/ml of two fold dilution for each extract were prepared and tested on each cell line, with three replicates for each concentration. The three extracts showed concentratio
... Show MoreThis study was carried out to study the toxic effect of smut wheat on male reproductive system of mice. The experimental study was performed in the laboratory using 20 mice, which divided into two groups, one group feed on normal diet, the other fed on a mixture of 50% normal diet with 50% smut wheat. A histopathological study was done on the testes of Mice fed on smut wheat for 30 days. The histopathological study revealed thickness of basement membrane of semiriferous tubule of mice, and desquamation of the sertoli cells and spermatogonium from the basement membrane of seminiferous tubule and has adversely effect on male fertility.
This study was carried out at the Iraqi ministry of science & Technology as well as the Vet. Collage- Baghdad. This research aimed to study the effects of macroscopic and histological licorice extract in the genital tract of ewes as well as to study the effect of the extract on reproductive efficiency to be compared with serum hormone PMSG( pregnant horse) used for this purpose. The study used (36) local ewes which are divided into three equal groups (T 1, T 2, T 3) (Licorice extract 300, PMSG 500 and zero respectively), The first group( T1) of ewes was given licorice extract a single dose of 300 mg / kg body weight weekly for a period of ( 6) weeks, The second group (T 2), they were treated with hormone PMSG with a dose of 500 unit
... Show MoreFifty isolates of Vibrio cholerae obtained from different areas in Iraq from patients with acute secretory diarrhea were diagnosed serologically in central public health laboratory(CPHL), Two different methods were used for detection cholera toxin production ,the first based on the degradation of Nicotin Amide Adinine Dinucleotide( NAD) by Cholera toxin (CT). and the second was by detection CT gene by polymerase chain reaction (PCR).All Vibrio cholerae were cultured in alkaline peptone water, out of 50 isolates 46 (92%) were positive when we used NAD for the detection of cholera toxin(CT).Whereas all isolates gave positive results by PCR. From these data we conclude that the degradation of NAD by CT is simple and can be carried out in sm
... Show MoreA total of (25) stool samples were collected from children and adults (2- 4) years old
suffering from diarrhea to isolate E. coli strains that produce heat-stable enterotoxin a (STa),
and after performing microscopic examination, cultural characterization and biochemical
identification only (11) isolates showed positive E. coli. STa activity was estimated by using
suckling mouse assay (SMA) and from these (11) isolates only (5) showed STa activity and
the one with the highest STa activity was selected for large scale production of STa, which
was followed by partial purification using ion-exchange chromatography (normal phase)
using DEAE sephadex A-50 column. After purification and determination of protein
concentr
The in vivo study was designed to evaluate the anticancer effects of these three types of the extracts on the AM-3 (Murine mammary adenocarcinoma). The LD50 was (5 g/kg, 6 g/kg and 8 g/kg) for ethanolic, hot aqueous and cold aqueous extracts respectively. The therapeutic doses of extracts were daily injected subcutaneously for two weeks, three weeks and four weeks. The histopathological study revealed pathological changes in some organs; the group that treated with ethanolic extract showed sever deposition of amyloid in some organs like spleen. The blood vessel filled with inflammatory cells as neutrophiles and cells infiltration in the lung tissue. Hot and cold aqueous extracts showed inflammatory mononuclear cells infiltration in the k
... Show MoreThe present study is designated to demonstrate the distinctive features, location, shape and boundaries, as well as the histological structures and the blood supply of the thyroid, parathyroid and ultimobranchial glands in the local breed turkey (Meleagris gallopavo). Twelve healthy birds from the local breed of Turkey were taken and divided into two equal groups: Six birds for anatomical study and six birds for histological study (three males and three females) for each study. Some anatomical parameters of the three glands which include: Length, width and volume, gross description, anatomical location and blood supply of the three glands were done. The anatomical results showed that the local breed Turkey has a pair of thyroid glands lo
... Show MoreBovine anaplasmosis is one of the tick-borne diseases caused by Anaplasma marginale bacteria which can cause high economic losses to livestock. Cattle that recovered from acute infection become carriers without clinical signs related to the disease and these bacteria can persist for lifetime in the blood. The present study was conducted to detect antibodies of persistently infected cattle with A. marginale in Wasit province/ Iraq. A total of 100 blood samples were collected randomly from cattle over one year old. Blood smears were prepared, stained with Giemsa,s stain and subjected to microscopic examination for detection of Anaplasma marginale bacteria within an infected RBCs, while serum samples were tested by a competitive enzyme - li
... Show MoreThe aim of this study was to investigate the effect of using milled Zingiber
Rhizome and vitamin E on some reproductivity traits (serum hormones levels, SSH and
testosterone and semen characters).
Eighteen Iraqi Black kids at age of 5-6 months were bought and divided into
three groups equally (body weight was considered), each group contained 6 kids. This
study was carried out in Animal's Farm, College of Veterinary Medicine, University of
Baghdad from 1st November 2007 up to end of May 2008, and treated as followed:
The first group (Z) (Zingiber group) was fed on concentrates diet (400 g/head)
daily which contained 10 g of zingiber (2.5% of diet). The second group (Z+E)
(Zingiber group + vit. E) was given the
The objjectt of tthiis sttudy was focused on ttransmiissiion of Liitteriia
monocyttogenes tthrough llayer body ,, tthe rolle of tthiis biirds iin tthe spread of tthiis bactteriia
and tthe effectt of experiimenttalllly iinfecttiion dose of tthe llayer oralllly on bactteriia
transmiitttted tto eggs ,,egg componentts and iintternall organs .. A ttottall of 45 llayer were
diisttriibutted iintto tthree groups and each group tto tthree replliicattes (15 llayer per replliicatte)
cosnsiistted of T1 (conttroll group free of iinfecttiion) ,, T2 and T3 were oralllly
experiimenttalllly iinfectted by 1x105 and 1x1010 (CFU \\ mll \\ hen of L.. monocyttogenes ..
Resulltts iindiicatted tthatt ..L.. monocyttogenes were iisollatted fr
This study was carried out at the animal station agriculture and the food
technology research center of Iraqi ministry of science & Technology. In a study to
determine the inhibitory effect of honey in the local growth of some pathogenic
bacteria in growth media (In vivo) and in laboratory animals (Invitro) was used four
types of bacteria brought from the Central Health Laboratory - Baghdad,
Escherichia coli , Streptococcus pneumoniae , Staphylococcus aureus , Streptococcus
pyogenus and Pasteurella hemolytica, and their biochemical characteristics were reexamined.
The study deals with studying the effect of local honey in the inhibition of
the growth of these bacteria on environmental Nutrient Agar (Invitro) t
The objects of this study were to determine the effect of Saccharomyces
cerevisiae cell wall (SCCW) to induce immunological response of Broiler chicks that
infected with Listeria monocytogenes . SCCW was administrated to chicks by adding
30 gm / 100 kg feed. A 30 broiler chicks were distributed into 3 treatments ( 10
chicks/ treatment) which were:
T1 : control group for both experiment fed on (SCCW). T2 : chicks fed 30 gm / 100 kg
feed (SCCW) and experimentally infected orally by Listeria monocytogenes 0.1 ml
of 1 × 109 CFU /ml. T3 : chicks were experimentally infected orally by Listeria
monocytogenes 0.1 ml of 1 × 109 CFU /ml. and study some immunological parameter
includes the concentration of - globuli
The aim of the present study is to investigate the antidiabetic effect of the aqueous extract of Momordica charantia seeds in alloxan - induced diabetic rats. Forty male albino Wistar rats were used, divided randomly into four groups (10 each). Oral administration of the seed extract at a dose of 150 mg/kg B.W. for 30 days showed a significant decrease in fasting blood glucose. Our results were greatly lower after oral administration of aqueous extract of Momordica charantia seeds in alloxan- induced diabetic groups than glibenclamide treated group. Also Momordica charantia extract and glibenclamide administered rats showed progressive increase in body weight
The aim of this work was to establish the vital role of using vitamin B complex and/or vitamin C in the reproduction. Twenty female rabbits were used and they were divided randomly into four equal groups, all animals were fed 100 g of concentrate pellets diet/ head. The first group was offered drinking water free of vitamins, (control group), second group was supplied with water containing vitamin C (40 mg/ 100ml water/ head), third group was supplied with water containing vitamin B complex (0.5 ml/100ml/head), and fourth group was water supplied containing 20 mg of vitamin C and 0.25 ml of vitamin B complex/100 ml water/head. At the beginning of the experiment, male rabbits were introduced to the females for mating for one hour at morni
... Show MoreIn order to investigate the phytochemicals presence in the seeds of local plant of Datura stramonium rather than alkaloids. For this purpose two types of Datura stramonium extract were prepared. The first was precipitated by acetone and the other was destructed by ether then precipitated with ammonium sulphate. The first extract yielded 2.5gram/ 5 gram of seeds weight; while, the second extract yielded 1.23 gram/ 5 gram of seed weight. Biochemical tests exhibited that lectin and trace amounts of glycosides were presented in the acetone precipitated extract. Whereas, the ether extract contained lectin, tannin, flavonoids, and trace amounts of alkaloids
The present study was designed to investigate the antinociceptive activity of seperate and
combined treatments of ethanol extract of Zingiber officinale rhizomes and vitamin C on
visceral nociception induced by intraperitoneal injection of 2% acetic acid which produced
writhing in rats. The preliminary chemical tests were performed on the extract and revealed the
presence of flavanoids, alkaloids and tannins. Visceral nociceptive responses including the
latency time to beginning of the first writhe, number of writhes per hour and the inhibition
percentage of writhing. Seperate i. p. injection of ethanol extract of Zingiber officinale at dose of
25 and 50 mg/kg BW caused significant (P<0.01) increase in latency tim