This study was conducted to extract and titrate the leukotoxin of Fusobacterium
necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in
Sulaimaniyah region. The culture supernatants of these isolates were subjected to the
tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F.
necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer
mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp.
funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).
Crimean-Congo hemorrhagic fever (CCHF) is a viral zoonotic disease with a high
mortality rate in humans. CCHF is caused by genus Nairovirus, in family of Bunyaviridae,
and is transmitted to humans through the bite of ticks Hyalomma spp or contact with blood or
tissues of CCHF patients or infected livestock.
The total numbers of positive patients to CCHF virus was 11 out of 44 suspected samples
were examined from eight provinces during the period from January to December 2010 . The
way of transmission is due to contact with blood and tissues of infected animals, and one
patient slaughtered sheep in his house. ELISA was used to detect Crimean-Congo
hemorrhagic fever (CCHF) virus-specific immunoglobulin M (IgM) in hu
A histological study was conducted to examine the structure of optic tectum in Clemmys caspica caspica. The results showed that the optic tectum consist of seven strata and the third stratum consists of three substrata, as follows: The stratum zonula (SZ), the stratum opticum (SO), the stratum griseum et fibrosum superficiale (SGFS) which consist of three substrata are a, b, c, the stratum griseum centrale (SGC), the stratum album centrale (SAC), the stratum griseum periventriculare (SGP), and the stratum album periventriculare (SAP). The stratum zonula (SZ) was the thinnest stratum in the optic tectum and its main thickness was (17-19) μm, while the stratum griseum et fibrosum superficiale (SGFS) was the thickest and its main thickness
... Show MoreThe present Study Showed that the falciform process and vitereal vessels is extended upper to retina, which are found only in S. triostegus . The visual cells in both fishes have cones and rods with myoid element, myoids are inconspicuous in S. triostegus . The cones in S. triostegus are large but in L. abu found in four types, long single cones, short single cones, dwarf cones and twin cones. It appears that the cones in L. abu arranged as mosaic include square and row patterns, but they are distributed randomly in S. triostegus , also the cones in L. abu have lateral fins which are lack in S. triostegus .The result showed that three types of ganglion cells, long, medium and small as well as there is additional type called Giant ganglio
... Show MoreThis study was conducted to isolate of Enterobacteriace family and designed to determine the prevalence of this bacteria in goats in 6 residance area in Baghdad Governorate ( Yousifia, Mahmodia, Shoulla, Sader city, Khadmia and farm of College of Veterinary Medicine ). 300 fecal samples were collacted from diarrheaic goats aged between (1 day to 2 year) from male and female for many month ,Diagnostic study depending upon the morphological and cultural properties of the isolates on some selective media in addItion to different biochemical tests and API-20E and serological tests isolates of Salmonella. The results showed Isolation of 111bacterial Isolates which included 6 different species of enterobacteriace, Citrobacte braackii )48)isola
... Show MoreThe present study was carried out to investigate the effect of oral administration of hot aqueous extract of beetle cocoon Larinus maculatus Faldermann, in a two doses 50
and100mg/Kg/Bw for 25 and 45days respect- tively on some organs such as liver, lung, kidney, intestine, heart, spleen, and brain in male mice Mus musculus. The results indicated that there were toxicopathological changes in many tissues of experimental animals. Histo -pathological changes was dose and period dependent . It was found that the aqueous extract of beetle cocoon has undesirable effect at the administered doses, since the raw extract of this cocoon is currently being used in Folk medicine as treatment for cough, bronchitis in Iraq. This study revealed that
This study was designed to investigate the correlation of various stress factors (PH, moisture contents, temp., and soil texture) on the ability of E.coli OI57:H7 to persist on/in soil on a dairy farm reared under field conditions at the college of Agriculture /University of Bagdad. The prevalence of E. coli O157:H7 in soil samples was determined for the period January to June 2012. The surface kinetics of E.coli O157:H7 onto the soil (surface kinetics), were theoretically achieved by dividing the farm into 3 zones starting from the fens (Z1),5m, and 10 m (Z5, and Z10, respectively) from the farm in three direction (right, left and rear of the farm) . While the depth kinetics were achie
... Show MoreThe study was designed to investigate the histomorphology of Miebomian gland in local Iraqi breed goats (coprus hircus).The research work included specimens of upper and lower eyelids of the eyes of 10 head of local breed black Iraqi goats aged 10-14 months (5 male and 5 female) and the routine histological technique was done from fixation till staining of histological sections. The study revealed that the Miebomian glands present in the sub palpebral conjnctiva arranged in parallel position along the upper and lower eyelids of the eye each gland opened besides the hair follicle (cilia) and its secretion are oily (lipid) called miebum. This material very important to spreading the tear on the cornea to p
... Show MoreThis study was performed on a 10 of local ewes during 24 hours after lambing. Five of the ewes suffering from uterine prolapse (treated group), and other five ewes didn't show uterine prolapse (control group). The study shows a relation between the calcium serum level and incidence of uterine prolapse after lambing. The result of study indicated that, a low level of calcium in the serum of treated group (2.29±0.33 mg/dl) as compared with the level of the calcium in the serum of the control group (6.52±0.30). The results indicated that the calcium deficiency (hypocalcaemia) in the late stage of pregnancy and at the lambing time may lead to uterine prolapse in ewes.
The present study is designed to evaluate two sutures pattern techniques (inverting and appositional) for urinary bladder closure following experimental cystotomy in dogs. Thirty two adult local breed males’ dogs were enrolled for this study, aged 1-3 years and weighing 18-23 kg. Dogs were randomly allocated into two equal groups. In the first group, cystotomies were closed via double-layers of continuous inverting suture pattern (Lambert and Cushing); while, in the second group, bladders incisions were closed by single-layer appositional suture pattern (simple interrupted) which did not involve the mucosa (extra-mucosal). Synthetic absorbable suture material polydiaxnone PDS 3/0 was used to close the bladder in all experimental animal
... Show MoreThe present study aimed to evaluate the effects of mercuric chloride (HgCl2) at different levels of biological organization in common carp Cyprinuscarpio following exposure for 30 days to a range of environmentally levels of mercuric chloride (0.01 and 0.02 mg L-1) and recovery for 3 weeks. Prior to evaluation of genetic damage (determined in erythrocytes using comet assay), enzymatic activity (ALT and AST), hematological parameters and histopathological examination of gill.The maximum tolerated concentration also determined which was found to be 1.63 µg l-1 above which complete mortality over the exposure period was observed. The results indicated that there was a strong induction
... Show MoreThis experiment was carried out at Animal Farm, College of Veterinary Medicine, Baghdad University, from Mar. up to Sept. 2010. Twenty eight Awassi ram lambs of 4-5 months old were equally divided into four groups of similar body weight and fed 2% of concentrate diet of body weight with grazing for 3-4 hours a day. The first group (C) and the third group (V) were fed concentrate diet free of cumin seeds; while the second group (CU) and the fourth group (CV) were fed the same amount of concentrate diet contain 3% of cumin seeds. All animals were grazed daily for 3-4 hours as one group. The third and the fourth groups were vasectomized by close vasectomy operation at the beginning of the experiment. The resul
... Show MoreA comparison between two techniques of subtotal laparoscopic cholecystectomy was studied to find the best technique for treating severe cholecystitis and to study the fate of the remaining part of the gallbladder. All experimental animals had inducing cholecystitis by clipping the cystic duct for five days which was enough for inducing cholecystitis in goats. 24 adult female goats were used in this study, which were divided randomly into two equal groups; subtotal laparoscopic cholecystectomy with cystic duct and cystic artery clipping by titanium clips (group A) and laparoscopic subtotal cholecystectomy with cystic duct clipping only (group B). Operations were performed under general anesthesia by using thiopent
... Show MoreThe aim of the present study is to investigate the antidiabetic effect of the aqueous extract of Momordica charantia seeds in alloxan - induced diabetic rats. Forty male albino Wistar rats were used, divided randomly into four groups (10 each). Oral administration of the seed extract at a dose of 150 mg/kg B.W. for 30 days showed a significant decrease in fasting blood glucose. Our results were greatly lower after oral administration of aqueous extract of Momordica charantia seeds in alloxan- induced diabetic groups than glibenclamide treated group. Also Momordica charantia extract and glibenclamide administered rats showed progressive increase in body weight
The aim of this work was to establish the vital role of using vitamin B complex and/or vitamin C in the reproduction. Twenty female rabbits were used and they were divided randomly into four equal groups, all animals were fed 100 g of concentrate pellets diet/ head. The first group was offered drinking water free of vitamins, (control group), second group was supplied with water containing vitamin C (40 mg/ 100ml water/ head), third group was supplied with water containing vitamin B complex (0.5 ml/100ml/head), and fourth group was water supplied containing 20 mg of vitamin C and 0.25 ml of vitamin B complex/100 ml water/head. At the beginning of the experiment, male rabbits were introduced to the females for mating for one hour at morni
... Show MoreIn order to investigate the phytochemicals presence in the seeds of local plant of Datura stramonium rather than alkaloids. For this purpose two types of Datura stramonium extract were prepared. The first was precipitated by acetone and the other was destructed by ether then precipitated with ammonium sulphate. The first extract yielded 2.5gram/ 5 gram of seeds weight; while, the second extract yielded 1.23 gram/ 5 gram of seed weight. Biochemical tests exhibited that lectin and trace amounts of glycosides were presented in the acetone precipitated extract. Whereas, the ether extract contained lectin, tannin, flavonoids, and trace amounts of alkaloids
The present study was designed to investigate the antinociceptive activity of seperate and
combined treatments of ethanol extract of Zingiber officinale rhizomes and vitamin C on
visceral nociception induced by intraperitoneal injection of 2% acetic acid which produced
writhing in rats. The preliminary chemical tests were performed on the extract and revealed the
presence of flavanoids, alkaloids and tannins. Visceral nociceptive responses including the
latency time to beginning of the first writhe, number of writhes per hour and the inhibition
percentage of writhing. Seperate i. p. injection of ethanol extract of Zingiber officinale at dose of
25 and 50 mg/kg BW caused significant (P<0.01) increase in latency tim
The aim of the present work was to determine the serum iron, total iron binding capacity (TIBC), unbound iron binding capacity (UIBC), transferrin saturation percentage (TS%) and Serum Copper in normal lactating and Ovine mastitis. The study was conducted on 50 clinically normal lactating and 50 ewes affected with staphylococcal mastitis (19 clinical and 31 subclinical), both groups aged 2-5 years in Salah Al-Din governorate. The blood samples were collected from jugular vein during the period October 2012 untill April 2013. The separated sera were directly used for measurement of investigated biochemical tests. The results showed that the ranges and means ± SE of normal lactating and Ovine mas
... Show MoreThis study was carried out to investigate the effect of the alcoholic extract of fenugreek seed (Trigonella foenum graecum) on thyroid gland function in adult male rabbits exposed to thiourea that induce hypothyroidism state. Thirty adult male rabbits weighing 1500-2000 gm were used, and randomly divided into five equal groups (6 rabbits/group) and treated daily for 42 days as follows: First group (control), (T1) group were treated with alcoholic extract of fenugreek seed(200mg/ kg .B.W. orally), (T2) group were treated with thiourea (10 mg/kg. B.W. orally), (T3) group were treated once thiourea daily by (10 mg/ kg. B.W) and after (3) hours given alcoholic extract of fenugreek 200 mg/kg B.W., and (T4) group were treated once thiourea dai
... Show MoreThe aim of the present study was to assess the effect of a commercial ß-glucan against acute toxic effect of copper sulfate on common carp Cyprinus carpio L. behavioral, hematological parameters and biochemical tests. A total of 250 Fish (150 ± 2 g) were fed a basal control diet or the basal diet plus a ß-glucan (1 g kg-1 diet) for 60 days. LC50 was calculated for 72 h which was 10.83 mg L-1. At the end of the trial, CuSO4.5H2O was added to all treatments at a dose of 5 mgL-1 for T1 and T2, 7mg L-1 for T3 and T4, 9 mgL-1 for T5 and T6 for 96h.
... Show MoreThe aim of this study was to conduct the effect of environmental conditions
(temperature degree and relative humidity) for three seasons ( winter , spring
and summer) on some physiological, productive and functional parameters of
Friesain dairy cow and their relation with two different lactational stages (
Second and third lactational stages). This study was carried out in Al-Tathamin
Dairy plan, Al-Dijyla'a ( Iraqi – Jordan Company) at Waist province. Thirty
dairy cows were selected and divided in two groups, each group contains fifteen
cows depended on lactation stage. Blood sample were collected at monthly
intervals for hematological examination viz; packed cell volume haemoglobin,
milk yield and milk trai
This study was conducted to demonstrate the effect of Metoprolol on chicken embryos during the period of cuddling automated .This study was completed in Babylon hatchery dedicated to the production of chick chickens, which is located in the province of Babylon. One hundred eggs (Belgian origin, CZ 1924) were divided into equal four groups. The first group injected eggs by Metaprolol at dose 10 mg /70kg BW, and the second group eggs was injected by Metoprolol at dose 15 mg /70kg BW, while eggs in the third group was injected by physiological normal saline only and the fourth group did not inject their eggs any material and considered the control group and all the eggs used in this experiment to take on the fourth
... Show MoreThe objjectt of tthiis sttudy was focused on ttransmiissiion of Liitteriia
monocyttogenes tthrough llayer body ,, tthe rolle of tthiis biirds iin tthe spread of tthiis bactteriia
and tthe effectt of experiimenttalllly iinfecttiion dose of tthe llayer oralllly on bactteriia
transmiitttted tto eggs ,,egg componentts and iintternall organs .. A ttottall of 45 llayer were
diisttriibutted iintto tthree groups and each group tto tthree replliicattes (15 llayer per replliicatte)
cosnsiistted of T1 (conttroll group free of iinfecttiion) ,, T2 and T3 were oralllly
experiimenttalllly iinfectted by 1x105 and 1x1010 (CFU \\ mll \\ hen of L.. monocyttogenes ..
Resulltts iindiicatted tthatt ..L.. monocyttogenes were iisollatted fr
This study was carried out at the animal station agriculture and the food
technology research center of Iraqi ministry of science & Technology. In a study to
determine the inhibitory effect of honey in the local growth of some pathogenic
bacteria in growth media (In vivo) and in laboratory animals (Invitro) was used four
types of bacteria brought from the Central Health Laboratory - Baghdad,
Escherichia coli , Streptococcus pneumoniae , Staphylococcus aureus , Streptococcus
pyogenus and Pasteurella hemolytica, and their biochemical characteristics were reexamined.
The study deals with studying the effect of local honey in the inhibition of
the growth of these bacteria on environmental Nutrient Agar (Invitro) t
The main objectives of this study was to determine the influence of soluble β-glucan extracted from the cell wall of Saccharomyces cerevisiae on immune response of broiler chickens reared under heat stress .β-glucan 225μg/ml was supplemented in drinking water to broiler chicken vaccinated with Newcastle disease virus (NDV) vaccine. The parameters of the assessment of the immune response was the Heterophil / Lymphocyte ratio as a measure of stress, determination of the serum antibody titer post vaccination with the NDV vaccine by ELISA test and Immunohistochemical detection of macrophages by using monoclonal antibodies (mouse antichicken macrophage KUL01). A hundred and twenty (120) Chickens one day old were divided into two equal grou
... Show MoreThis study was performed to identify gross and histopathology changes which can be occur in testis and spermatic cord following injection of Acrylic Dental Base Material inside spermatic cord , the agent is widely present ,cheep, quick ,highly solid and used as a substance of chemical castration. It was injected inside spermatic cord of 15 healthy adult bucks in the ratio of 3:1 bilaterally ; Treated animals where divided randomly in to 5 groups depending on time for gross and histopathology examination after injected with the material. Animals were followed up for 7 days, 14 days, 30 days, 60 days, and 150 days after injection. Result showed good and immediate hardening after 20-25 min. of injected material. Gross examination of testis
... Show MoreThis study was conducted to find out the effect of licorice extract and eCG on the some
reproductive traits in Awassi ram lambs. This study was conducted on State Board for Agriculture
Researches, Animal Researches station. Twenty four Awassi ram lambs at the age of 67-77 days
were randomly divided into four groups according to the lambing date as follow: Group (A) was
injected with eCG 300 I.U /lamb/week. Group (B) was treated with licorice 300 mg/kg B.W/week
orally. Group(C) also was treated licorice 400 mg/kg B.W/week orally. Group (D) were kept as a
control group. Testes measurements were taken at the 12th week of the study period. The level of
testosterone hormone was measured at monthly intervals. Age of
The aim of this study was to detect the effect of toxoplasmosis on some liver enzyme activity
such as bilirubin concentration, Alanine aminotransferase (ALT), Aspartate aminotransferase
(AST), Alkaline Phosphatase (ALP) in serum. Twenty hundred and fifty women were examining
by Enzyme-linked immunosorbent assay (ELISA). One hundred and thirty-four were infected with
toxoplasmosis with different number of abortion (1-4) times were used in this work. All Serum
ALT and AST activities were increased and ALP and total bilirubin level decreased in infected
women below the normal value. The analysis of the plasma enzyme distribution pattern suggests
that liver cell damage occurred during virulent and low virulent inf
This study was conducted to isolate and identify the fungi that infect burn wounds in humans and
acquired wounds or wounds resulted from surgical operation in farm animals. A total of 110 cotton
swabs, 60 cotton swabs were collected from wounds to 6 kinds of farm animals, which include 10
swabs for each type of these animals (cattle, sheep, goats, cats, dogs, donkeys). As well as 50 swabs
were collected from human burns. All these samples were cultured onto Sabouraud dextrose agar
(oxoid, England) to detect fungi in wounds of humans and animals in Baghdad city.Identification of
Yeasts were done by use RapID™ Yeast Plus System (remel, USA), While the Identification of
moulds were done in the Central Public Hea
Colonies of E.coli O157:H7 were isolated from 35 raw milk sample and their identification were confirmed based on biochemical reactions and both cultural and serological characteristics. Presumptive E.coli O157:H7 isolates obtained by selective plating on both CT-SMAC and Chromogenic agars were further tested serologically for the presence of both O157 and H7 antigenes using the commercial available latex agglutination test kit. The unhygienic practices in the production of milk in Al-Thahab Al- Abiedh , Abu-Graib, Al-Zedan and Khan Dharie were reflected on the highest significant (p<0.01)prevalence level of contamination with E.coli O157:H7 that appeared to be 80%, 80%, 60% and60% respectively. Homogenization pressure of 1000 psi and
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