This study was aimed to test four types of different plants for the purpose of selecting the optimal plant as a source of the amylase enzyme, including wheat, barley, rice, and potatoes. Among these plants, wheat was selected as the best plant source for enzyme extraction because it possessed the highest effectiveness of the enzyme specific activity (3.0 U/mg protein). Furthermore, sodium acetate buffer (0.2 M, pH 6.0) was determined to be the optimal extraction buffer with a 1:5 (w:v) ratio after 75 min, and it provided 68 U/mg protein. The enzyme was concentrated with sucrose before being purified by gel filtration chromatography using Sephadex G-150. The results demonstrated a 1.8-fold increases in final purification folds wit
... Show MoreFreshwater algae (Chlorophyceae-except Zygnematales) were collected from 18 sites (12 springs and 6 streams) in Shaqlawa district within Erbil Province, Kurdistan Region of Iraq from September-2019 to August-2020. Aim of this study isolation and identification new algal species. 27 new algal genera with 40 species records at first time in Kurdistan Region and others provinces of Iraq, which include the following, Scenedesmus considered the most abundance than others algae which seven species identified followed by genus of Chlamydomonas, genus of Chlorophyceae, genus of Characium with three species for each one recorded, but genera Geminella have two species, finally the remaining gene
... Show MoreThis study was aimed to examine and detect the total flavonoid presence from the crude extract use in phytochemical test and HPLC device, and detecting on both extract by UV-spectrophotometer. The objective of this study for inhibition the pyocyanin pigment that produce by pseudomonas aeruginosa by use Marticaria chamomilla crude and flavonoid extract. The extraction of chamomile was accomplished by methanol 80%, where different concentrations and dilutions of the crude and flavonoid extract and used as anti-virulence factor. 130 clinical samples of burns and wounds where collected from patients in the medical city hospital. The result showed that 44 isolates where identified as P.aeruginos
... Show MoreMeropenem (MER) antibiotic is removed from an aqueous solution using Mg-Al-layered double hydroxide (Mg/Al-LDHs) which serves to functionalize the principal object of enquiry in this paper–sunflower husks (SFH). The former sample demonstrated a 51% removal of MER while the latter demonstrated a 67% removal of MER. One of the areas explored was the impact of the rise calcination temperatures resulted in a concurrent increase in the surface area and porosity of LDHs which, in turn, provoked a heightened removal efficacy of MER at calcination temperatures between 300 °C and 350 °C. Nonetheless, LDHs experienced structural deformation and thus, diminished removal efficacy of MER when the ca
... Show MoreThe current research dealt with a comparative taxonomic study of the two types Chenopodium album L. and Chenopodiastrum murale L. at Baghdad , This research was included a cytological study by calculating the chromosome number by sequence n=27,9. and studying the micro-morphological characteristics of the seeds and fruits of the two types, in addition to the surface covering in the leaf part. All phenotypic characteristics of the seeds were studied by scanning electron microscope (SEM) and Light microscope (LM) in terms of shape, colour, dimensions Seed size were measured by the program digmizer , as well as the configuration of the seeds surface , The seeds of Ch.albu
... Show MoreThis experiment was aimed to evaluate and compare the effects of adding natural extracts (clove, Mentha and thyme) with synthetic BHT on some physiochemical, microbial, and sensory characteristics of lamb patties stored at 4 Cº for 12 days. Results indicated that there’s a steady rise (P<0.01) in oxidative rancidity, microbial and pH with the increasing time of storage. Also, a significant (P<0.01) reduction in TBA, microbial and pH was observed in patties treated with clove, thyme, mentha and BHT as compared with untreated samples. It was found that adding natural extracts is more effective in inhibiting microbial count than did BHT. The highest score of overall acceptability was n
... Show MoreOptimal growth of shrimp and harvest in a pond is strongly influenced by water quality because this is a common thing but it cannot be denied that it is also an important thing. The combination of physicochemical parameters and biological indicators has become a classic way of studying water quality. Phytoplankton is a bioindicator that affects the productivity of vannamei shrimp in ponds. Currently, shrimp farming activities are intensive. This study aimed to analyze the diversity and abundance of phytoplankton and water quality in vannamei shrimp ponds. The research objective was achieved by calculating the abundance, diversity index, evenness index, and plankton dominance index in ponds. The results of the study were that the
... Show More A Restricted Selection Index was constructed to genetically evaluate animals using a dataset composed of body weights of Shami kids at the ages of birth, weaning, and 6-month. The variance-covariance matrix (VCV) was generated and used to estimate genetic parameters for the index construction after adjusting the fixed effects; year, birth month, kid sex, type (single, twin or triplet) of birth, doe age, weights at kidding (KW), birth (BWT) and weaning (WWT) as covariates. General Linear Model and Restricted Maximum Likelihood methods were used to estimate BLUE for fixed effects and variance components for random effects, respectively. BW was 3.6
This work was conducted to study the association of variations in the VLDLR gene and mRNA expression in the liver and the ovary of local Iraqi hens with some productive and blood traits. The VLDLR gene variations were studied using blood samples of 173 pedigreed hens, 47 weeks old while the expression was studied using tissue samples of the ovary and liver in 30 pedigreed hens, 60 weeks old. Genotypic and allelic frequencies, production traits, and biochemical serum blood were investigated. The results showed that the genotypic and allelic frequencies of the VLDLR in 173 chickens did not agree with Hardy-Weinberg equilibrium (P<0.0307). The genotypic frequencies were 94.7 and 5.3% for both GG and GT genotypes respectively, whi
... Show MoreThis study was conducted to investigate certain characteristics of the purified Phenylalanine ammonia lyase (PAL). The results revealed that the molecular weight was 180 kDa by gel filtration and Native-PAGE. The optimal pH and temperature for enzyme activity were 7 and 40 °C respectively. The enzyme activity was stable against pH values range of 6-8. The enzyme retained 79% of its total activity after incubation for one hour at 50 °C and retained 7% (lost 93%) of its activity at 70 °C. The entire activity was completely lost when the enzyme was incubated for 1 hour at temperatures over 70 °C. Carbohydrate content of the purified enzyme was estimated to be 24.61%. Km, Kcat and Vmax were estimated with phenylalanine as substra
... Show MoreA field experiment was carried out at Agricultural Researchs and the Experiments Station in Wasit province during 2019-2020 season. This study was aimed to investigate the effect of three factors, the first being biofertilizer at three levels (without addition , a combination of Bacillus megaterium + Glomus mosseae and complet combination of G. mosseae, Azotobacter chroococcum, B. megaterium and Azospirillum brasilense). the second factor was three levels of vermicompost (without,3,6 Mg ha-1).The third factor included three levels of triple superphosphate (0, 40 and 80%) of the fertilizer recommendation. The treatment were distributed in a factorial experiment according to a randomized complete block
... Show MoreThis study was performed to determine whether active optical sensors could develop an algorithm for N recommendation for the potato crop (Solanum tuberosum L.). The experiment was conducted in Maine State, (USA) during the growing season of 2018-2019. Six N rates (0-280 kg ha-1) were applied on eleven locations under a randomized complete block design (RCBD), with four replications. Data of normalized difference vegetation index-(NDVI) were collected via active sensors, GreenSeeker-(GS), and Crop Circle-(CC). Sensors measurements collected at the 20th of the leaf stage were significantly associated with tuber yield, where the exponential model exhibited a b
... Show MoreThis study was aimed to evaluate the Aqua program in calibration and validity and its use in simulation to study the productive and water characteristics of maize crop.. The experiment was conducted in two irrigation methods, sprinkler irrigation and surface irrigation, and two cultivation methods are borders (lines) and furrows (lines and then furrows) and for two cultivars of maize, a hybrid (unlocal) and a local variety. The results that sprinkler irrigation reduced water consumption compared to surface irrigation, as it reached (558.38) mm for sprinkler irrigation, and (668.79) mm for surface irrigation. While the method of furrows after borders outperformed the borders only in improving growth characteristics and increasing
... Show MoreThis study was aimed to investigate the involvement of Myeloid Related Protein 14 (MRP14) in the production of CXC Motif Chemokine Ligand 9 (CXCL9) in thyroiditis patients. The first group composed of 27 hypothyroidism (HO) patients (12 men and 15 women), age average about 46 years old. The second group had 43 patients (8 men and 35 women) with hyperthyroidism (HR), their mean of age was 42, while 30 healthy persons act as control group (HC) had an average age of 33.47 of men (13) and women (17). The three groups were testing for triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) furthermore, testing the thyroglobulin (TG), anti-thyroperoxidase (a-TPO), anti-TSH receptor (TSHR) and serum level of MRP14 by
... Show MoreThis study was aimed to find the most prevalent microbial isolates, as well as measurement of total serum IgE, LPS levels and their correlation with Atopic dermatitis occurrences. A 88 patients were collected randomly (47 females and 41 males), through a period Sep. 2019 to Feb. 2020 from Al Zafaraniyah General Hospital. Patients ages ranged between 10 months to 30 years. In addition, the control samples are collected randomly from 20 apparently healthy people. Microbial isolation results showed that the most prevalence microbial isolates in atopic skin patients were; Staphylococcus spp. 81 (30.10%), fungi 65 (24.20%), Bacillus spp. 53 (19.70%), Enterobacteriaceae spp. 32 (11.90%), Aci
... Show MoreThere is an elevated need for novel antimicrobial preservatives in the food industry, and hydrolysis of waste products from the same sector has for decades been viewed as a potential source of these. In the current study we have purified bovine whey protein using size-exclusion chromatography (SEC), and hydrolyzed it by trypsin, pepsin, alcalase, savinase, and neutrase at different times 30, 60, 120, 240, and 300 min. The highest active time hydrolysate was subsequently fractionated by SEC, monitored for antibacterial and antioxidant activities, and characterized using UHPLC-MS/MS. Alcalase and savinase displayed higher degree of hydrolysis, higher antibacterial activity in their hydrolysates at 60 and 30 min, respectively compar
... Show MoreThis study was aimed to identify the histological and histochemical composition of the Ileum in the Slender-billed gull (Chroicocephalus genei). It was using histological stains and histological chemical techniques. The results showed that the Ileum was relatively long, so it characterized into two parts: an anterior part and a posterior part. The results showed that the mucous layer in the two parts consisted of villi that appeared conical in the anterior region and conical to a triangle in the posterior part, and the villi were more numerous and longer in the anterior part. The villi covered with a simple columnar epithelial tissue containing goblet cells in the two regions, but the goblet cells were significantly more
... Show MoreThis study was aimed description of morphological features of fruits and seeds of eight species related to Brassicaceae family in Iraq, the study showed number of taxonomic differences as the silique was long in all studied except the species Rorippa amphibian (L.)b Besser was short, however the fruits varied in dimensions where the species Turritis laxa L. recorded the highest average length with (160 mm) while the species R .amphibia was record the shortest length (5 mm), in addition the colors ranged from green to brown. This study showed that all of the species was glabrous except Arabis caucasia Willd. was lanute-woolly. The configuration shapes were linear with exc
... Show MoreThis study was aimed to isolation of pathogenic bacteria from different clinical cases like burns, wounds &UTI infections, then study the antimicrobial activity of Laurus nobilis leaves water extract on it. From a total of (80) samples were taken from these cases, the most isolated bacteria were related to Pseudomonas aeruginosa, Klebsiella pneumoniae, Staph aureus & Escherichia coli. Antibiotic sensitivity test was done for isolated bacteria against (9) antibiotic and most of them revealed sensitivity to gentamycin, ciprofloxacin& trimethoprim/ sulphamethoxazole. Different concentration of Laurus nobilis leaves water extract (25, 50, 100,
... Show MoreThis study was aimed evaluation, Zinc sulfide-chitosan nanoparticles (ZnS-chitosan NPs) as an antibacterial agent. The nanoparticles of Zinc sulfide-chitosan were synthesized using a single-step colloidal process. Different factors were optimized, which included pH, temperature, reaction time, concentrations of chitosan and Zinc chloride .The optimal conditions was achieved at pH 7, temperature 60℃, reaction time 60min, with 0.04 mg/ml of Zinc chloride, 2.5 ml 0.1mg/ml Sodium sulfide and 0.009 M chitosan. The size of ZnS-chitosan NPs size was tested by using FESEM which were 35nm, surface morphology was done by using AFM. Moreover, X-ray Diffraction (XRD) characterized the crystal s
... Show MoreThis study aims to determine the vitelogenesis activity by FSH hormone induction in the early stage of gonadal maturation. The treatament is a differences FSH dose with indicators of vitelogenesis activity were estradiol-17β levels in the blood, Hepatosomatic index (HSI) and Gonadosomatic Index (GSI). The study used a treatment A : Dose FSH at 1.0 ml/kg; B: Dose FSH at 1.25 ml/kg; C : Dose FSH at 1.5 ml/kg; control: nothing induction. The results showed that there was an effect of FSH dose on the estradiol 17β levels, HSI and GSI, there ongoing vitelogenesis activity. The trend of the GSI and HSI has a close correlation to levels of estradiol 17β, with the best dose of the hormone is 1.25 ml/kg resulting levels of esradiol 17
... Show MoreThis study was aimed to characterize Roseomonas mucosa isolate using molecular genetics and determination some bacterial virulence factors such as capsular, biofilm formation and antimicrobial susceptibility. By using Polymerase chain reaction (PCR) technique, the molecular characterization of the R. mucosa was done by using 16S rRNA gene sequence and phylogenetic analysis. Antimicrobial susceptibility test was carried out by Kirby-Bauer disk diffusion method. Capsular detection was examined using nigrosin pigment. While Congo red agar and microtiter plate using enzyme-linked immunosorbent assay (ELISA) reader methods are used to determined bacterial isolate capability to produce a biofilm. The molecular charact
... Show MoreA field experiment was conducted at the Grdarasha Research Field station, College of Agricultural Engineering Sciences, Salahaddin University – Erbil using randomized complete block design (RCBD) during the years 2019-2021 to study the effect of five levels 0, 5, 10, 15 and 20 g of dust metals of Erbil steel factory (size 2mm mesh) in combination with compost of solid waste management and sorting of Akre district-Duhok province on growth, yield and chemical composition of Gundelia tournefortii L. The results indicated that powdery metal dust and compost fertilizer significantly increased length, diameter, fresh weight and dry weight of edible portion, and thus escalated the total yield of this plant for the two growing
... Show MoreTow field experiments were conducted during the seasons of 2020/2021 and 2021/2022 at the experimental farm Etay El-Baroud research station to investigate the effect of foliar application with gibberellic acid (GA3) at 50 and 100 ppm and the chitosan (CS) at of 100 and 200 ppm, in addition, to control for each to Giza Red onion. The results showed significant and positive effects of the examined concentrations of GA3 or CS on all the studied characters except leaves number and shape index, in both seasons. Concerning the interaction effect, all of the studied characters were enhanced significantly in all treatment combinations between GA3 and CS levels. The treatments of GA3 (50 ppm) with CS (200
... Show MoreThis study was aimed to evaluate the effect of supplementing fenugreek seed oil to the diet on the physiological, anatomical and histological characteristics of broilers. A total of 200 chicks one day-old of (Ross308) were used in this study and divided into 4 treatments, 5 replicate for each treatment by 10 chicks in each replicate. The treatments were T1(control diet) = without additives (basal diet), T2 = basal diet + 0.25 ml fenugreek oil / kg, T3 = basal diet + 0.50 ml fenugreek oil / kg and T4 = basal diet + 0.75 ml fenugreek oil /kg. The results showed Fenugreek oil inclusion at levels 0.50 ml/kg exhibited significant decrease (P <0.05) in jejunum and ileum relat
... Show MoreThe present study was aimed to detect the accumulative effect of polycyclic Aromatic Hydrocarbon (PAHs) in Arabibarbus grypus collecting along the Tigris River Southern of Baghdad City. A total of 130 specimens were taken from three sites in Al-Zafaranyia City from the period between Summer 2020 until Spring 2021.Results of statistical analysis for total PAHs at S3 showed a significant increase (P ≤ 0.05) compared to S1 and S2 for all seasons. Results revealed that the total concentrations of PAHs in both muscles and gills in
The current study conducted to identify the bacterial isolates from burns and wound infections that are responsible for various skin complication in such conditions and determination the development of antimicrobial resistance among these bacterial isolates. This study is carried out Between September 2020 to December 2020, a total number of (103) burns and wound swabs were taken from different patients from different hospitals in Baghdad city, the specimens were collected from; burns (57) and wound (46) swabs, the specimens were included; (51) female and (52) Male, the age average between (2 - 70 years). The positive bacterial containing specimens are (93) swabs, most of them are mixed (82 specimens) with differ
... Show MoreThis study was aimed to estimate the antioxidants, antibacterial, and antifungal activities of methanolic extract from soybean seeds against pathogenic microorganisms. The methanolic extract was prepared from defatted soybean flour. Total phenolics content (TPC), total flavonoid content (TFC), and the major phenolic, flavonoid, and isoflavone compounds in soybean extract were estimated by HPLC. On the other hand, biological activities such as antioxidants, antibacterial, and antifungal activities were evaluated. The value of phenolic compounds was 8.59 mg GAE/g extract, while the value of flavonoids was 0.82 mg QE/g extract. The chemicals listed below were chromatographed and identified: syringic acid, quercetin, gallic acid, ben
... Show MoreThis experiment was aimed to study the effect of the interaction of herbicides and varieties with different types of foliar nutrition in production the highest yield for three wheat varieties. A field experiment was carried out at the College of Agricultural Engineering Sciences - University of Baghdad / Jadiriyah for two seasons, as the seeds of the first season were sown on 13/12/2018 and harvested on 15/5/2019, and the seeds of the second season were sown on 4/12/2019 and harvested on 10/5/2020. The randomized complete block design (RCBD) was used in the arrangement of split plots, the nutrition included the main plots, while the varieties with herbicides treatments occupied the secondary plots with three replications. The tre
... Show MoreThis research was aimed to analyze the impact of the Iraqi dinar exchange rates for the period 1990-2020 on the imported quantities of rice crop, and to measure the price elasticity of demand for this crop. The (ADRL) model was applied to measure the impact of these prices in addition to the (international price).After conducting the statistical tests (Dickie Fuller test) to examine the stability of the time series of the studied variables included in the model, which stabilized after taking the first difference for them, the results of the analysis showed that international prices had a weak effect on the imported quantities of rice crop, and that these quantities increased at an annual growth rate 6% more than the increase in t
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