The aim of this study is to investigate the effect of nano fertilizers and application method on growth and yield of date palm. The experiment was carried out at AL- Rabeaa date palms station - Department of Horticulture - Ministry of Agriculture on 10 years old of Khastawi date palm cultivar during 2016 - 2017. Factorial experiment (3×6) within randomized complete block design with three replications .carried out using two factors: the first was three application methods; foliar application, injection with trunk and soil fertilization (A1, A2, A3) respectively. The second factor was different types of fertilizer, seaweed extract of the nanotechnology product with two concentrations: first (F1) (1,0.5,2)ml.L^-1and the second (F2) (2,1,4) ml.L^-1 respectively, depending on the method of application and optimus-plus fertilizer the product of nanotechnology with two concentrations: first (F3) (1,0.5,2) ml.L^-1 and the second (F4) (2,1,4) ml.L^-1 respectively depending on the method of application also used NPK fertilizers (F5) (2,1,4) g.L^-1 and control treatment (F0).The results showed that treatment (F1) was increased fruit weight (5.26 g). While (F2) produced 36.75 kg yield. The highest percentage of maturity appeared in (F3) (73.56%). Treatments F4 and F5 showed an increase of amino acids in fruits (9.68 and 10.88 mg^-1 dry weight).injection treatment superior in the percentage of dry matter in leaves (38.60%), chlorophyll content (32.19 mg, 100 g^-1),and yield (36.07 kg). we could be concluded that using nutrients and injection fertilizer improved vegetative growth and increase yield of date palm.