Trichoderma harzianum produced 5.383 U/mg of laccase in solid state fermentation (SSF) using 10gm of wheat bran as substrate with moisture content 1:0.7 w/v, using  4 plugs×5mm as inoculums size, for 7 days at 28C°. Laccase was partially purified with 7.260 fold and yield 84.01% by ion exchange chromatography DEAE-cellulose. Partially purified laccase had an optimum pH of 4 and was stable in pH range from 3.5 to 5.5. The optimum temperature for laccase activity was 35C° and stability was range from 15 to 35C° with 1h of incubation. The ability of T. harzianum isolate to decolorize textile dyes on solid media appears completely decolorization of Blue dye with concentrations 50,100 and pa

The PRNCR1 gene variant among sample of Prostate Cancer (PCa) Iraqi patients case-control study was used, with PCa patients (n=32) and healthy individual control (n=33). It was successfully identified two SNPs in prostate cancer-associated noncoding RNA-1 (PRNCR-1) gene by using PCR technique and direct sequencing . The first SNP is rs13252298 (A>G) have three genotypes (AA,AG,GG )and the second SNP is rs5013678 (T> C) have three genotypes TT,TC,CC),these genotype frequencies were not in agreement with Hardy-Weinberg Equilibrium (HWE) in patients and controls .It was observed that AG genotype  was more frequent in patients than controls(

 This study was aimed to development and modification the method of analysis for active ingredient in Tamsulosin drug by using high performance liquid chromatography (HPLC) also determined shelf life and storage conditions for Tamsulosin. Chromatographic conditions utilized stationary phase C18 (250*4.6) mobile phase 0.05Nmixture of  di hydrogen ortho phosphate and Acetonitrile 55:45 , preserve the flow rate near (1ml/min) and length of wave has been 275nm, The retention time found to be 10 minute for the HPLC process . The Tamsulosin assay result was found to be 99.93% . The calibration curve linearity analysis results showed a strong linear relationship in the concentration range (10-

This study was aimed to detect the spreading of E.histolytica among children with diarrhea, to achieve that,221 stool specimens were collected from diarrheal children of all ages and both gender, in Baghdad city at a period extended from early September 2019 to the end of February 2020. The collected specimens were examined directly by the light microscope for detecting the presence of E.histolytica. DNA was extracted from positive microscopically stool specimens, then<

In this study out of 130 different clinical specimens, 70 Acinetobacter baumannii were collected from hospitalized patients. Then; all the isolates were characterized using biochemical tests and confirmed by using the Vitek II compact system. All isolates of Acinetobacter baumannii were subjected to the disc diffusion method to determine it's a susceptibility to 8 types of antibiotic disc, and the result showed these bacteria were resistant to Cefepime, Imipenem, trimethoprim, Piperacillin, tetracycline, Amikacin, ampicillin-sulbactam and levofloxacin. According to bacterial susceptibility test, all isolated were showed highly resistance to Trimethoprim (88.57%) and the lower resistance to tetracycline

Extraction of Copper ion from its water solutions using plant waste (compost), sludge and Bentonite metal as cheap natural adsorbed materials. It was found that Copper ion extraction was based on concentration of  Copper  and amount of the adsorbed materials. Highest amount of adsorption was obtained at 180 µg Cu ml^-1 as Bentonite, plant waste and sludge respectively. Thermal equilibrium of the system was studied by Langmuir equation, R² gave a good linear relationship  in succession. Maximum regulatory capacity and regulatory capacity at equilibrium is 1.0 , 0.99 and 0.96 to  plant waste, sludge and Bentonite respectively, as the preference coefficient

This study was aimed to measure marketing efficiency and study important factors affecting , using TOBIT qualitative response model for wheat crop in Salahalddin province. Results revealed that independent factors such as (marketing type, crops duration in the field, average marketing cost, distance between farm and marketing center, and average productivity) had an impact on wheat marketing efficiency. This impact varied in size and direction due to value of parameters. Values of marketing efficiency fluctuated within cities and towns in the province. The average value on the province level was 76.75%. This study was recommended developing marketing infrastructures which is essential to efficiency increases. In addition, it is i

A field experiment was conducted on a gypsiferous sandy clay loam soil to examine the effects of blending Triple superphosphate (TSP) with urea on N, P concentrations in plant and growth parameters of broad bean. The experiment was a factorial randomized complete block design (RCBD) with three replicates. The first factor was type of application as briquettes which include T1 (one layer of TSP between two layers of urea) and T2 (one layer of urea between two layers of TSP), the second factor was application depth (5 and 10 cm D1 and D2), and the third factor was application rate (1.0, 1.25, and 1.50 as much as N and P fertilizer recommended for broad bean, R1, R2 and R3). Broad bean was planted and the following growth parameters

The research aims to study the profit functions of vegetable crops. Cross-sectional data was used based on a random sample that included 200 farmers in Alqosh region within Nineveh Province for the 2018-2019 agricultural season. The results showed that there are vegetable crops which need a large amount of labor as labor costs amounted to about 71% of the total costs per acre in tomato production. It was also found that total costs were higher in tomato crop cultivation at the dunum level compared to other vegetable crops. In addition, profit function shows that the price of the product has a significant effect on profit compared to the rest of the independent variables in the functions of vegeta

The research aims to identify the reality of the extension services provided to workers in the vegetable production in the farms of Imam Hussein and Al-Abbas’s shrines by the agricultural and extension departments in the holy Karbala governorate. The research included the vegetable farms belonging to Imam Hussein and Al-Abbas’s holy shrines, as well as the agricultural extension departments that provided extension services to those farms. The research sample was chosen from all the agricultural extension agents in the agricultural extension departments in Karbala governorate, totaling (36) agricultural extension agents. In order to achieve the objectives of the research, the researcher prepared a questionnair

This research was aimed to study and analyze the costs, revenues, and profits of meat chicken breeding projects. The study also estimating the random frontier profit function, the profit inefficiency function and profit efficiency which based on cross-sectional data that included 59 projects for raising meat poultry in Waist governorate in 2020.The results of the analysis showed through the descriptive analysis of the cost structure that, the costs of chicks and fodder account for more than 70% of the costs, as well as achieving economic profits for projects. Through the random border profit function, the significance of both the total revenue and the costs of chicks, fodder, medicines, vaccines, and services (water, electricity

This research was aimed to study the exposure of Razzazah Lake  to major hydrological changes in recent years as a result of natural climatic changes  and  drought, high evaporation in lake due to stop discharge from Habbaniyah Lake  by Al- majera channel. During 2019, we collected surface water samples at three locations, and three samples from groundwater, in addition one samples from each location Imam Ali Drop and Sewage water  of Karbala. The Results  show that the heavy isotopes in lake and groundwater well are enriched during the warm period, and depleted during the cold period. Chemically, The dominant cations and anions in Al-Razzaza lake water are mainly of

This study was aimed to investigate the occurrence of chloritization phenomenon in these soils. Three of these pedons were chosen in soil sites differing in their agricultural exploitation, which included: one pedon in an uncultivated soil at Daratw . While the second pedon was dug in soil exploited by the cultivation of  wheat at Bardarash . The third pedon was chosen as representative of a forest soil at Tawska Valley. Also, another two soil pedons were exposed to successive cycles of wet and dry, were chosen in Aski Kalak , and  Altun Kupri districts. All studied soils were examined by X-ray diffractions. Results showed the presence of Mica, Smectite, Real chlorite and Kaolinite mine

A study was conducted to evaluate the quality of Diyala River water with some heavy metals (Pb, Cu, Ni, Cd), Boron and their effect on pollution of agricultural soil surrounding river that is irrigated with river water. Nine areas located on river within Al-Jisr district of Al-Madain district, southeast of Baghdad Governorate / Iraq, were selected, namely Umm Al-Ubaid, Al-Uwaija, Al-Khafaji, Al-Kargouliya, Jurf Al-Naddaf, Al-Samadiyah, Abu Thila, Al-Arifiyah, Al-Tuwaitha, and northern shores of Salman Pak, for comparison purposes. Water samples were taken for three months during summer (June, July, and August) and three months during the winter (January, February, and March) of 2024, and average values ​​for two seasons were

A set of 60 samples were collected from different sources of multiple areas of Hyderabad (India) in order to select a local Escherichia coli isolates that have the ability to produce HSP70. 26 isolates were characterized after studied the morphological characteristics and microscopic properties of the isolates on macConkey agar and EMB recorded after 24hr. and grams staining was done. The isolated bacteria were screened for various biochemical tests to get 11 isolates that submitted for molecular identification, and the amplification of 16S rRNA regions using universal primer (16S rDNA) verified only 5 E. coli isolates which were sequenced and NCBI accession numbers were acquired. Five distinct isolates (RU-1, R

This research aimed to form a molecularly imprinted polymer by adding aluminum ion at the first step were to the allyl bromide monomer resulted was formation using bulk polymerization. To acquire the highest adsorption capacity, molar ratios of template, monomer, and cross-linking agent, as well as solvents and multiple monomers, were investigated. Scanning electron microscopy (SEM) was used to analyze the produced aluminum. the maximum adsorption capacity of Al-IIP were 0.1531 µmol/g and 0.2362 µmol/g respectively. aluminum adsorption followed a Langmuir isotherm model. Solid-phase extraction (SPE) syringe packed with ionic imprinted polymers (IIP) were used to selective separation and preconcentration for alu

This study was conducted to detecting the FASN gene polymorphism and its relationship to daily milk yield (DMY), lactation period, and milk composition in addition to polymorphism distribution and allele frequency in 52 Awassi ewes and their lambs. It was carried out from 5/1/2022 to 30/10/2022 at the Sheep Farm of the Al-Fayhaa station in the Jableh sub-district / Al-Musaib project (55 km south of Baghdad), in addition to the Biotechnology Laboratory at the College of Agricultural Engineering Sciences/University of Baghdad. Three polymorphism appeared in this variant (A>G SNP), which are AA, AG, and GG, with the percentages of 63.46, 32.69, and 3.85%, respectively. The variation among them were highly significant (P≤0.

This study was aimed to evaluate detection of T. gondii in blood DNA based on the B1 gene and serologically, also to investigate the effect of some risk factors (sex, ages, breeds and flocks). A total of 190 goats were included. The blood samples were obtained from the jugular vein and subjected to nested PCR (nPCR) and Elisa for detection of T. gondii. The results obtained that the prevalence was 24.21% by nPCR and 46.31% by ELISA with significant differences. The degree of agreement between nPCR and ELISA was a slight (Kappa=<

This study was conducted to estimate (Co) variance components of a sexual and egg production traits in Iraqi brown local chickens. Data on 2234 pedigreed hens for successive six generations of selection for high egg production during the first 24 weeks of laying were used. Data of first egg weight (FEWT), body weight at sexual maturity (BWSM), age at sexual maturity (ASM), egg number (EN), egg weight (EW) and egg mass (EM) were recorded individually. The univariate and bivariate animal models under Average Information Restricted Maximum Likelihood (AI-REML) were applied to estimate the variance component and the BLUP breeding values. The average of the traits mentioned above was 35.7g, 1489.59g, 149.31d, 109.32 egg/hen, 44.80g an

This study aimed to examine how including dietary dried rosemary leaves (DRL) of Holstein bulls affects methane production and certain rumen fluid characteristics in vitro. Rumen fluid was obtained from newly slaughtered calves and used in two separate trials: one for gas production and another for digestion. In the trials, two diets were implemented. The first was the standard diet for bulls, consisting of a concentrate diet and alfalfa hay (control group). The second diet included the addition of 250 g DRL to the essential diet. There was a significant decrease (P≤0.01) in methane and N-NH3 production, as well as in the ratio between unsaturated fatty acids (USF) and saturated fatty acids in the rosemary group compar

The current study was aimed to ascertain the impact of ciprofloxacin, an acidifier acid, and a Bacillus subtilis on immunological function and growth efficiency after a starter-phase challenge with Salmonella pullorum. 250 broiler chicks (Ross 308) were put into five groups of fifty each, all groups except the fifth group, orally challenged with S. pullorum at one day old, and provided with a supplement in drinking water after three days as follow G1: treated with B. subtilis (2×10⁷ CFU/g) (250 g/1000 L). G2: treated with acidifier acid (0.5-1 ml/L). G3: treated with ciprofloxacin 10% (0.5 ml/L). G4: only infected with S. pullorum. G5: control negative. Th

This study was conducted to investigate the polymorphism of ACTN3/EXON-19 gene and their relationship the performance of local horses in Iraq. This study was carried out at the Iraqi Equestrian Club (15 km west from Baghdad city center) period during d from 1 February to 30 December 2022, to investigate the gene polymorphism of ACTN3 region EXON-19 and their relationship with performance of local horses in Iraq. Were found three variants (3 SNPs) in the ACTN3 gene - exon 19 and in different locations, namely rs1148960207, whose genotypes were CC, CT, and TT, as their distribution rates were significantly different (P≤0.05).

This study investigates the efficiency of three types of flat fan nozzles (blue, red, and brown) when operating with a boom sprayer under low-volume spraying conditions in the Gorno Botevo village area of Central Bulgaria. The experiment evaluated the sprayer's operational speed and working pressure across eight working regimes, focusing on the impact of speed and nozzle type on application performance. Statistical analyses, including univariate ANOVA and correlation analysis, revealed significant differences in performance among the nozzles. The blue, red, and brown nozzles achieved optimal performance at 8.5 km/h, 12 km/h, and 16 km/h, respectively, while maint

This study was conducted at two locations, Qlyasan Agricultural Research Station and Kanipanka Nursery Station, during the 2023–2024 winter season to investigate the effects of organic fertilizer and stubble height on forage yield attributes of grass pea genotypes. A split-split plot design was used with three factors: fertilizer application (F0= no fertilizer, F1= organic fertilizer), genotypes (G1: Local, G2: IGC-2011-62, G3: IGC-2011-35, G4: IGC-2011-9), and stubble height (SH1= 5cm, SH2 = 10cm). Fertilizer application significantly increased DFY in Kanipanka. Genotype significantly influenced most forage yield attributes, with G1 showing the highest values. Stubble height significantly affected GFY and DFY, particularly in

The aim of this study was to investigate the role of some hydrocolloids in improving the physical and sensory properties of gluten-free bread manufactured from millet flour. Whole grain millet flour (T2), whole grain millet flour with xanthan gum (T3), whole grain millet flour with guar gum (T4), unprocessed millet flour with extraction rate of (71%) with xanthan gum (T5), unprocessed millet flour with extraction rate of (71%) with guar gum (T6) ,were used  to prepare gluten free bread beside another treatment manufactured by the same ingredients with replacing the sugar by glycerol (T7).The wheat flour was used as standard (T1).  The results of the chemical composition showed that whole-grain Proso millet flour had hig

This study was aimed to identification of certain crosses to produce hybrids with higher performance per se can be aided by the determination of simple sequence repeats (SSR), which can improve our understanding of the genetic divergence of maize lines and their classification into different heterotic groups. Variability for each locus was measured using the polymorphism information content (PIC), with an average of 0.55, suggesting that the markers were highly informative. Analysis of the molecular variance (AMOVA) indicated higher divergence among the maize lines, suggesting the existence of different groups. The unweighted pair group method with arithmetic mean analysis (UPGMA) and th

This research aims to determine the effects and limitations of using B. purpurea in complete feed on the performance of male Kacang goats. The study involved sixteen male Kacang goats aged 10-12 months with a weight range of 12-14 kg. The research method employed a Completely Randomized Design with four treatments and four replications, consisting of T0: G. sepium 30% + Natural grass 38% + Rice bran 7% + Milled corn 24,8% + Mineral mix 0,2% (control); T1: G. sepium 30% + Natural grass 38% + Rice bran 6,5% + Milled corn 22,8% + Mineral mix 0,2% + B.

This study was carried out to identify the effect of the quantitative trait loci (QTL) region on some productive and physiological traits of Iraqi chickens. One hundred five and ten-week-old laying hens were used and bred in individual sages from sexual maturity to 100 days of production. Blood samples were collected from the brachial vein for DNA extraction and serum isolation, and then polymerase chain reaction (PCR) was done to determine the target region of the QTL. Two groups were obtained: the first had a PCR product with band 294 bp denoted (P) and the second had no PCR product denoted (NP). The P group had a significant (p < 0.05) effect on egg weight at the second stage of the study amounted 48.23 gm. and a significan

Since phosphorus (P) is lower in agricultural areas with acid sandy soil, it runs out in different parts of crop plants after harvesting. To boost growth and development in the next cropping system, only overuse of inorganic phosphate fertilizers can adversely affect agricultural sustainability. Phosphate-solubilizing fungi like Candida tropicalis play a role in plant P nutrition through soil P dynamics and growth hormones. Three PSF isolates (Sw01, Pu02, and Pu04) from bulk soil of fresh corn were tested for optimal media and incubation times. The results showed that Sw01 produced the highest population of 593.33×10⁸CFU L⁻¹ when cultured<

This study aimed to estimate variability and some genetic parameters for yield and yield components in pea (Pisum sativum L.) genotypes under different phosphorous levels. A field experiment was carried out at the field of Horticulture Department, College of Agricultural Engineering Sciences, Duhok University, on the 1^st of November 2021. the experiment unites layout according to split-plot arrangement, the main plots include phosphorus levels (0, 18, 36, and 54 kg ha^-1 P₂O₅ and the subplot represented the thirteen genotypes within RCBD. The results exhibited that phosphorus levels were highly significant for all traits except biological yield, also the genotyp