Extracellular Histones Inhibit Complement Activation through Interacting with Complement Component 4

Yasir B. Qaddoori; Simon T. Abrams; Paul Mould; Yasir Alhamdi; Stephen E. Christmas; Guozheng Wang; Cheng-Hock Toh

doi:10.4049/jimmunol.1700779

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Publication Date

Fri Jun 15 2018

Journal Name

The Journal Of Immunology

Volume

200

DOI

10.4049/jimmunol.1700779

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Extracellular Histones Inhibit Complement Activation through Interacting with Complement Component 4

Yasir B. Qaddoori

Simon T. Abrams

Paul Mould

Yasir Alhamdi

Stephen E. Christmas

Guozheng Wang

Cheng-Hock Toh

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Abstract<p>Complement activation leads to membrane attack complex formation, which can lyse not only pathogens but also host cells. Histones can be released from the lysed or damaged cells and serve as a major type of damage-associated molecular pattern, but their effects on the complement system are not clear. In this study, we pulled down two major proteins from human serum using histone-conjugated beads: one was C-reactive protein and the other was C4, as identified by mass spectrometry. In surface plasmon resonance analysis, histone H3 and H4 showed stronger binding to C4 than other histones, with KD around 1 nM. The interaction did not affect C4 cleavage to C4a and C4b. Because histones bind to C4b, a component of C3 and C5 convertases, their activities were significantly inhibited in the presence of histones. Although it is not clear whether the inhibition was achieved through blocking C3 and C5 convertase assembly or just through reducing their activity, the outcome was that both classical and mannose-binding lectin pathways were dramatically inhibited. Using a high concentration of C4 protein, histone-suppressed complement activity could not be fully restored, indicating C4 is not the only target of histones in those pathways. In contrast, the alternative pathway was almost spared, but the overall complement activity activated by zymosan was inhibited by histones. Therefore, we believe that histones inhibiting complement activation is a natural feedback mechanism to prevent the excessive injury of host cells.</p>

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Publication Date

Sat Dec 11 2021

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

Association of Age, Parity and Body mass Index with Hemoglobin and Serum Ferritin Levels in Pregnant Women in Baghdad City

Anemia

Body mass index

Hemoglobin

Ferritin

Parity

Mayada M.

Ali Abdulhussain

Rawaa Dawood

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Hemogloin (Hb) and serum ferritin levels are used to assess anemia in pregnancy. Some studies referred to the influence of maternal age, body mass index (BMI) and parity on Hb and serum ferritin levels. The study aimed to examine the possible association of maternal Hb and serum ferritin with maternal age, parity, and BMI in a sample of pregnant women in Baghdad.

Ninety healthy pregnant women, grouped in three equal groups according to the pregnancy trimester, and thirty apparently healthy non-pregnant women from Baghdad were enrolled in this observational study. Blood and serum samples were obtained for the estimation of Hb and serum ferritin levels.

The pooled data of participants showed a n

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Publication Date

Thu Jan 06 2022

Journal Name

Iraqi National Journal Of Nursing Specialties

Effectiveness of Self-Regulation Fluid Program on Patients with Hemodialysis Self-Efficacy for Fluid Adherence in Al-Diwaniyah Teaching Hospital

Self-regulation

Hemodialysis

Self-efficacy

Fluid

Adherence

Alaa Hamza Hermis

Rajaa Ibrahim Abed

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Objectives: to evaluate patient knowledge with hemodialysis and to determine the effectiveness of Self-regulation Fluid Program on Patients with hemodialysis self-efficacy for fluid adherence in Al-Diwaniyah Teaching Hospital.

Methodology: A quasi-experimental design (two group design: pre-test and post-test) was used. This study was conducted in Al-Diwaniya Teaching Hospital for the period from (15^th of October 2018 to 20^th of May 2019) on a non-probability (purposive) sample consisting of (60 patients) treatment in hemodialysis units. A questionnaire was built as a data collection tool and consisted of four parts:

First part: Demographic characteristics of the pati

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Publication Date

Thu Mar 05 2020

Journal Name

International Journal Of Psychosocial Rehabilitation

Special exercises with ropes, rubber bullets and their impact in some biomechanical variables in the 100m hurdles ran for juniors

Ali

Sarmad

Intisar

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Publication Date

Sat Jan 31 2015

Journal Name

Journal Of Al-qadisiyah For Pure Science

synthesis ,spectral studies and antimicrobial evalution of 6,6-(1,2-benzenediazo)bis(3-amino benzoic acid) complexes with some metal ions

Dhafir

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International Journal Of Applied Engineering & Technology

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Hind Suhail Abdulhay

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Publication Date

Fri Sep 01 2023

Journal Name

Iranian Journal Of Veterinary Science And Technology

Investigation of bacterial contamination with Klebsilla and E. coli in the prepucal cavity of pubertal and adult age in caprine

Klebsiella

Escherichia coli

Prepucal cavity

Pubertal age

Adult age

Goat

Mohammed A.K.

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Publication Date

Mon Jan 01 2024

Journal Name

Clinical Laboratory

Histopathological Tracing of HPV Genotypes 6 and 11 in Pediatric Patients in Medical City with Chronic Palatine and Pharyngeal Tonsillitis

Ahmed

Shamil

Saad

Shakir

Athraa

Khalil

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(1)

Publication Date

Thu Oct 25 2018

Journal Name

Al-nahrain Journal Of Science

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