Background/Aim: Capsicum annuum Grossum Senise, a widely cultivated sweet pepper in Iraq contains a diverse array of bioactive compounds such as polyphenols, carotenoids and capsinoids, known for their beneficial effects on health. The study aimed to employ advanced analytical techniques to elucidate the compound's chemical properties and concentration within this specific Capsicum cultivar, contributing to the growing body of knowledge regarding plant-derived sterols with potential nutraceutical application. Methods: This study focused on the extraction, isolation, purification and quantitative analysis of b-sitosterol from Iraqi Capsicum annuum Grossum. Advanced chromatographic and spectroscopic techniques were employed to ensure accurate quantification and structural characterisation. Results: The chloroform fraction exhibited a high b-sitosterol concentration (0.492 mg/mL dry weight), suggesting efficient biosynthesis of this secondary metabolite, possibly due to favourable biochemical and environmental factors. Conclusion: The findings underscore the potential of Iraqi-grown Capsicum annuum Grossum as a valuable source of b-sitosterol, supporting its use in nutraceutical and pharmaceutical applications. Further research is warranted to explore its mechanistic pathways and therapeutic efficacy in clinical settings.
