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Fifteen local isolates of Pseudomonas were obtained from several sources such as soil, water
and some high-fat foods (Meat, olives, coconuts, etc.). The ability of isolates to produce lipase
was measured by the size of clear zone on Tween 20 solid medium and by measuring the
enzymatic activity and specific activity. Isolate M3 (as named in this study) was found to be
the most efficient for the production of the lipase with enzymatic activity reached 56.6 U/ml
and specific activity of 305.94 U/mg. This isolate was identified through genetic analysis of the
16S rRNA gene. and it was shown that the isolate M3 belongs to Pseudomonas aeruginosa
with 99% similarity. The DNA of isolate M3 was extracted and lipase gene was amplified
through PCR tec
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