ABSTRACT Possible interference of vamin nutritional solution with the activity of several B-lactam antibiotics against E.coli was evaluated in vitro.In Minimal basal salts-glucose medium rapid growth inhibition of sensitive E. coli was induced by 4 µg/ml of ampicillin / cloxaillin, 8 µg/ml of ampicillin, 6 µg/ml of carbencillin, hostacillin, and cephalotin, and by 32 µg/ml of penicillin G and cloxacillin. Significant inactivation of up to 32 µg/ml of carbencillin, cephalotin, penicillin G, and hostacillin was induced by addition of 1:20 v/v vamin. This inactivation was due to the presence of specific amino acids in the mixture. Deletions of amino acids revealed that valine, leucine, isoleucine, tyrosine, tryptophan, phenylalanine, cysteine, methionine, asparagine, or threonine were essential for such inactivation while the other ami no acids (alanine, glycine, aspartic acid, argenine, glutamine, glutamic acid, histidine, serine, proline, and lycine) were not Furthermore, the presence of the three branched chain amino acids ( Valine, leucine, and isoleucine ) was essential to inactivate cephalotin and carbencillin. While the aromatic amino acids ( Tryptophan, phenylalanine and tyrosine) were essential to However, cephalotin. inactivate cloxacillin, and ampicillin / cloxacillin inactivated by any amino acid treatment ampicillin, were not These results suggest that certain B-lactam antibiotics undergo significant decrease in activity when sensitive E. coli is treated with vamin and this effect is due to the presence of specific amino acid in the solution
Aflatoxin B1 (AFB1) is a mycotoxin produced mainly by fungus Aspergillus flavus in food and feed . It is considered as a carcinogenic toxin for human and animals. The current study was designed for produce antibody (IgG) against aflatoxin B1.It was achieved by immunization of experimental animals (New Zealand White rabbits) with prepared antigen consist of aflatoxin B1-BSA Conjugate (100 and 200 μg ) concentrations, and detection of produced antibody using Ouchterlony double immunodiffusion and ELISA techniques,. Ochterlony and ELISA techniques revealed that, high titer of IgG antibody was obtained by rabbit’s immunize, and the titer of antibody was increased steadily during the immunization schedule. The highest titer of antibody rea
... Show MoreCrude-colicin was extracted from g.coli isolated from urinary tract
infection patients. Toxicity of various concentrations of crudt: colicin
on the viability of immune cells [phagocytic ceUs(M0,PMN) and lymphocytes] was studied. Results indicated that toxicity ciTect of crude colicin is dependent on concentration. Low concentrations (I0,
25, 50 mg/ml) didn't have toxic effects, but when there was an increase in the consternation of this extract, the viability of the immune cell s began to decrease.
In this study, only four isolates produce CNF1 from 76 isolatesof uropathogenic Escherichia coli.cnf1 gene was detected by using PCR technique, while cytotoxic necrotizing factor 1(CNF1) was determined by Immunoblotting assay.
Twenty-seven S. aureus isolates were obtained from patients referring various hospitals in Baghdad. Only 17 isolates produced DNase. SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar. Nevertheless, only those phenotypically-producer of DNase harboured nuc gene. Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column. SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight. A matter indicates that the enzyme is consistently pure. Results proved that SNase was able to significantly (P< 0.05) reduce th
... Show MoreThis research focuses on the characteristics of polyvinyl alcohol and starch polymer blends doping with Rhodamine-B. The polymer blends were prepared using the solution cast method, which comprises 1:1(wt. /wt.). The polymer blends of PVA and starch with had different ratios of glycerin 0, 25, 30, 35, and 40 % wt. The ratio of 30% wt of glycerin was found to be the most suitable mechanical properties by strength and elasticity. The polymer blend of 1:1 wt ratios of starch/PVA and 30% wt of glycerin were doped with different ratios of Rhoda mine-B dye 0, 1, 2, 3, 4, 5, and 6% wt and the electrical properties of doping biodegradable blends were studied. The ratio of Rhodamine-B 5% wt to the polymer blends showed hi
... Show MoreStaphylococcal enterotoxin B (SEB) is a potent superantigen produced by
Lead toxicity elicits neurological damage which is a well-known disorder that has been considered to be a major cause for multiple condition such as behavioral defect; mental retardation; and nerve insufficient activity.
This research is designed to estimate potential protective effect of vinpocetine on neurotoxicity stimulated by lead acetate in rats.
Eighteen adult rats of both sexes were randomly enrolled into three groups. Each group includes 6 rats as followings: Group I- Rats were given 0.3ml normal saline solution orally; then intraperitoneal injection of 100μl of the normal saline was given 1h later; this group was considered as control. Group II- Rats were given an intraperitoneal injection of 20mg/kg lead acetate
... Show MoreIn this paper, a new class of nonconvex sets and functions called strongly -convex sets and strongly -convex functions are introduced. This class is considered as a natural extension of strongly -convex sets and functions introduced in the literature. Some basic and differentiability properties related to strongly -convex functions are discussed. As an application to optimization problems, some optimality properties of constrained optimization problems are proved. In these optimization problems, either the objective function or the inequality constraints functions are strongly -convex.
So muchinformation keeps on being digitized and stored in several forms, web pages, scientific articles, books, etc. so the mission of discovering information has become more and more challenging. The requirement for new IT devices to retrieve and arrange these vastamounts of informationaregrowing step by step. Furthermore, platforms of e-learning are developing to meet the intended needsof students.
The aim of this article is to utilize machine learning to determine the appropriate actions that support the learning procedure and the Latent Dirichlet Allocation (LDA) so as to find the topics contained in the connections proposed in a learning session. Ourpurpose is also to introduce a course which moves toward the student's attempts a
In the partition theory, there is more then one form of representation of dedication, most notably the Abacus diagram, which gives an accurate and specific description. In the year 2019, Mahmood and Mahmood presented the idea of merging more than two plans, and then the following question was raised: Is the process of separating any somewhat large diagram into smaller schemes possible? The general formula to split e-abacus diagram into two or more equal or unequal parts was achieved in this study now.