Studying the Effectiveness of Bromelain Enzyme Extracted from Pineapple Against Anti-Inflammatory and Anti-Cancer Cells

S A Mohamad; Z T Sedrah

doi:10.1088/1755-1315/1252/1/012158

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Publication Date

Fri Dec 01 2023

Journal Name

Iop Conference Series: Earth And Environmental Science

Volume

1252

DOI

10.1088/1755-1315/1252/1/012158

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Studying the Effectiveness of Bromelain Enzyme Extracted from Pineapple Against Anti-Inflammatory and Anti-Cancer Cells

S A Mohamad

Z T Sedrah

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AbstractThe experiment was carried out in the laboratories of the College of Agricultural Engineering Sciences, University of Baghdad, Bromelain enzyme was extracted from pineapple fruit crown and pineapple peel. An extraction solution was used with different mixing ratios from 1:1 to 5:1. The mixing ratio of 2:1 was the best extraction ratio, as it reached the highest enzymatic activity among the other mixing ratios, which was 33 units. The enzymatic activity of the crown extract was higher than the enzymatic activity of the peel extract, reaching 9.8 units/ml and 1.1 units/ml, respectively, the enzyme was precipitated using ammonium sulfate at a concentration of 30 % - 90 %. This concentration 60% is (total activity 77 units) The enzyme’s anti- inflammatory activity was tested. The enzyme’s anti-cancer activity was tested after selecting two single breast tissue-derived human cancer cell lines, MDA-MD 231 and MCF-7

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Publication Date

Thu Nov 19 2020

Journal Name

Indonesian Journal Of Chemistry

Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using p-Amino-N,N-dimethylaniline as a Derivatizing Agent

dispersive liquid-liquid microextraction

derivatizing agent

eugenol

p-amino-N

N-dimethylaniline

Bahaa Malik

Omar

Sarmad Bahjat

Keith Edward

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Two simple methods for the determination of eugenol were developed. The first depends on the oxidative coupling of eugenol with p-amino-N,N-dimethylaniline (PADA) in the presence of K3[Fe(CN)6]. A linear regression calibration plot for eugenol was constructed at 600 nm, within a concentration range of 0.25-2.50 μg.mL–1 and a correlation coefficient (r) value of 0.9988. The limits of detection (LOD) and quantitation (LOQ) were 0.086 and 0.284 μg.mL–1, respectively. The second method is based on the dispersive liquid-liquid microextraction of the derivatized oxidative coupling product of eugenol with PADA. Under the optimized extraction procedure, the extracted colored product was determined spectrophotometrically at 618 nm. A l

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