Multiple sclerosis (MS) is a chronic, inflammatory demyelinating disease of central nervous system with complex etiopathogenesis that impacts young adults (Lee et al., 2015), and MS impacts younger and middle aged character and leads to a range of disabilities that can alter their daily routines (Yara et al, 2010). Although, the exact cause of MS is still undetermined, the disease is mediated by adaptive immunity through the infiltration of T cells into the central nervous system (Bjelobaba et al, 2017). MS causes the Focal neurological symptomsand biochemical changes in the molecular level and the variation of neural cells such as loss or alteration of sensation, motor function, visible signs such as blurred vision or transient blindness, disturbance of conjugate eye movements, bladder and bowel dysfunction and cognitive impairment (Induruwa et al, 2012 and Jafarzadeh et al, 2014). Autoimmune diseases (ADs) are chronic conditions initiated by way of the loss of immunological tolerance to self-antigens (TodorovicDilas et al, 2011). It is a heterogeneous group of disorders in which more than one modification in the immune system can be specific to a particular tissue or organ or might also be systemic, non-specific, involving multiple tissues or organs (Ray et al, 2012). One possible cause behind this is a lack of understanding of pathogenic mechanisms driving progressive multiple sclerosis. Due to the indolent nature of symptom progression, current disease criteria used to signify the course of disease (Lublin et al, 2014) indicate diagnosis is generally retrospective and based totally on history of gradual worsening. Clearly, diagnosis is primary based on clinical judgment, as there is no fully reliable diagnostic test (Ontaneda et al, 2015). In latest years, the elements involved in the etiology of the disease have also included oxidative stress (OS), which is described as an imbalance between the generation of reactive oxygen species (ROS) and the mechanisms that are responsible for their elimination, andthe imbalance between OS agents and antioxidants leads to OS activating the inflammatory process (Phaniendra et al, 2015). In the absence of enough antioxidant defenses, ROS can reason oxidative damage to macromolecules resulting in oxidation of lipids, proteins and deoxyribonucleic acid (DNA) (Griffiths, 2002). Some reseach report that ROS play a main role in myelin phagocytosis (Ghabaee et al, 2010 and Tasset et al, 2012). The inflammatory response gives rise to the manufacturing of both ROS and Reactive Biochem. Cell. Arch. Vol. 19, No. 1, pp. 31-35, 2019 www.connectjournals.com/bca ISSN 0972-5075 Nitrogen Species RNS through monocyte interactions with brain endothelium; ROS manufacturing induces cytoskeletal rearrangements, loss of blood-brain blood BBB integrity, tight-junction alteration and the extravasation of leukocytes into the central nervous system (Van et al, 2011; Witherick et al, 2011). Aim of study The aim of this study focuses on determination 8-H2-dG, MDA and PON1 in multiple sclerosis disease and finds the relationship between newly marker 8-H-2-dG with MDA and PON1. MATERIALS AND METHODS Subjects This study was performed on 25 female patients with age (25-35) years who diagnosed by physicians as a multiple sclerosis in Misan governorate. The patients compared with 25 apparently healthful in the identic rangel of age. In this study sample was collected five mL of venous bloods, placed in to plain tubes until coagulation was performed. Serum was separated from blood cells by centrifugation 4000 r.p.m. Assay method Determination of serum of 8-H-2-dG This assay that can be used for quantification of 8- H-2-dG in urine, cell culture, plasma and other sample matrices. The ELISA utilize an 8-H-2-dG coated plate and HRP- conjugated antibody or detection which allows for any assay range of 0.94-60 ng/mL, with sensitivity of 0.59 ng/mL. Determination of MDA The concentration of MDA,which is the consequence of lipid peroxidation and a marker of oxidative stress, was measured using thiobarbiturc acid. Determination of PON1 The quantitive sandwich enzyme immunoassay (ELISA) technique was employed for determination of PON1.
Gestational diabetes mellitus is glucose intolerance of varying degree with onset or first detection duringpregnancy,it can causelong and short term morbidities in both the mother and the child, such as shoulder dystocia,preeclampsia, and high blood pressure. The most powerful endogenous vasoconstrictor peptide, urotensin II, andits receptor are involved in the etiology of gestational diabetes mellitus.Aim of the study: The study’s goal was to see if there is a link between Urotensin II levels and insulin resistancein pregnant women with gestational diabetes.Patients and method: A case-control study that was conducted in obstetrics and gynecology department atBaghdad Teaching hospital from the first of January 2019 to the end of D
... Show More
Coupling reaction of 2-amino benzoic acid with the 8-hydroxy quinoline gave the azo ligand (H2L): 5-(2-benzoic acid azo )-8-hydroxy quinoline.Treatment of this ligand with some metal ions (CoII, NiII and CuII ) in ethanolic medium with a (1:2) (M:L) ratio yielded a series of neutral complexes with general Formula[M(HL)2],where: M=Co(II), Ni(II) and Cu(II), HL=anion azo ligand (-1).The prepared complexes were characterized using flame atomic absorption,FT-IR and UV-Vis spectroscopic methods as well as magnetic susceptibility and conductivity measurements.
The aim of the present study is to highlight the role of total cholesterol (TC), triacylglycerol (TG), Glycated hemoglobin A1c and iron in Iraqi women with multiple sclerosis and also to examine the biochemical action of copaxone (which is the most widely used in the 21st century to treat multiple sclerosis) on these biochemical parameters. This is the first study in Iraq which deals copaxone action on TC , TG , HbA1c and iron. Ninety women in their fourth decade suffering from multiple sclerosis were enrolled in this study. They were divided into: the first (group B) composed of (30) women without any treatment related to multiple sclerosis or any treatment linked with chronic or inflammatory diseases. The second (group A1) included (30)
... Show More
A series of new coumarin and N-amino-2-quinolone derivatives have been synthesized. The reaction of coumarin (1) with excess of Hydrazine hydrate 98% yielded 1-amino-2-quinolone (2), Compound (2) was reacted with different Sulfonyl chloride to yield Sulfonamides [ N-(2-oxoquinolin-1(2H)-yl) methane sulfonamide (3), N-(2-oxoquinolin-1(2H)-yl) Benzene sulfonamide (4) and 4-methyl-N-(2-oxoquinolin-1(2H)-yl) benzene sulfonamide (5) ], while reaction of 2-(4-methyl-2-oxo-2H-chromen-7-yloxy) acetic acid (8) with different amines yielded compounds [ 2-(4-methyl-2-oxo-2H-chromen-7-yloxy)-N-(2-oxoquinolin-1(2H)-yl) acetamide (9) and N-(5-methyl-1,3,4-thiadiazol-2-yl)-2-(4-methyl-2-oxo-2H-chromen-7-yloxy)acetamide (10) ] th
... Show More
(1)
Background: Coronavirus disease 2019 (COVID-19) is an emerging zoonotic disease caused by the new respiratory virus SARS-CoV2. It has a tropism in the lung tissues where excess target receptors exist. Periostin plays a role in subepithelial fibrosis associated with bronchial asthma. Since the Coronavirus's target is the human respiratory system, Periostin has been recently described as a valuable new biomarker in the diagnosis and evaluation of disease in patients with COVID-19 lung involvement. Objectives: To assess the level of Periostin in the serum of COVID-19 patients and to correlate its role in disease severity and prognosis. Subjects and Methods: Periostin serum levels were measured for 63 patients attending three main COVID
... Show More
(1)
The objective of the study: is to investigate the correlations between the HER2 neu gene status with the clinicopathological parameters of infiltrative breast carcinoma. A total of seventy four Iraqi breast cancer patients were collected from one center (Department of Public Health) paraffin blocks were collected from histopathology department central public health laboratories, Bagdad, Iraq from 2014-2015. The cases which has been taken included invasive ductal and invasive lobular carcinoma type Women age were ranged from 24-80 years old. Evaluation of Her-2/neu gene amplification status was done using FISH and CISH techniques that showed a significant correlations with clinicopathological parameters.
New simple and sensitive spectrophotometric methods for the determination of paracetamol in aqueous medium were developed. The first method is based on coupling of paracetamol with p-amino-2-hydroxy sodium benzoate (AHB) in the presence of sodium periodate, as oxidizing agent, to form a brownish-orange compound which shows a λmax at 470 nm. The molar absorptivity (εmax) of the colored product was found to be (3371) l. mole1. cm-1 and Sandel’s index 0.0449 μg. cm-2. The method follows Beer’s law in the concentration range of 12.5-500.0 μg of paracetamol in a final volume of 25 ml (0.5-20.0) μg. ml-1 with relative standard deviation percent (RSD%) ranged between 0.26-4.71% and accuracy, expressed by recovery percent, 95-106% for five
... Show More4-amino-3-(4-(((4-hydroxy-3, 5dimethoxybenzyl) oxy) methyl) phenyl)-1, 2, 4-triazole-5-thione was synthesized by to method the first one from melt reaction of 4-(((4-hydroxy-3, 5-dimethoxybenzyl) oxy) methyl) benzoic acid with Thiocarbonyldihydrazide, the second method from convert the corresponded acid hydrazide to potassium 2-(4-(((4-hydroxy-3, 5-dimethoxybenzyl) oxy) methyl) benzoyl) hydrazinecarbodithioate salt then react with hydrazine hydrate. Newly Schiff base (7a-7f) were synthesized from reaction the 4-amino-1, 2, 4-triazol with substituted hydroxybenzaldehyde. The resulting compounds were characterized by IR, 1H-NMR, 13C-NMR, and HRMS data. 2, 2-Diphenyl-1-picrylhydrazide (DPPH) and ferric reducing antioxidant power (FRAP) assays
... Show MoreAnkylosing spondylitis (AS) is a common, highly heritable inflammatory arthritis affecting primarily the spine and pelvis. This study was aimed to investigate the relationship between the rs27044 polymorphism in Endoplasmic reticulum aminopeptidase-1 (ERAP-1) with the susceptibility and severity of AS correlated with some biochemical markers such as hematological parameter (Erythrocytes sedimentation rate (ESR)) and immunological parameters (C-reactive protein (CRP), Human leukocyte antigen-B27 (HLA-B27), Interlukin-6 (IL-6) and Interlukin-23 (IL-23)), and oxidative stress parameters (Glutathione (GSH) and Malondialdehyde (MDA)) in a sample of Iraqi population. A total of 60 blood samples were collected from AS patients requited Rhe
... Show More