Preferred Language
Articles
/
wBiY4ZQBVTCNdQwCXSE3
Isolation, optimization, and redesigning of phages of methicillin-resistant Staphylococcus aureus from clinical hospital isolates in Baghdad
...Show More Authors

Background: A global health concern is methicillin-resistant Staphylococcus aureus (MRSA). The use of bacteriophages is one of the many novel control strategies against MRSA that are frequently sought. However, it is quite challenging to isolate enough lytic anti-MRSA phages. In order to extract, optimize, and remodel anti-MRSA phages, this study sought novel approaches.

Methods: Two ATCC MRSA strains and nine clinical MRSA isolates were used to isolate wild anti-MRSA phages from hospital settings, dirt, and sewage. The wild phages were optimized using plaque-based biokinetic techniques. Using chemicals that weakened bacterial cell walls, the resulting highly lytic and specific anti-MRSA phages were subjected to unique physicochemical phage redesign processes. This allowed the phages to enter host bacteria and acquire the specificity of the new host. Three different protocols were tested using combinations of Tween 20, lysozyme, and nisin A.

Results: Nisin A and lysozyme protocols at different rates were found to be successful in producing newly redesigned, transiently stable, anti-MRSA phages.

Conclusion: Unlike self-depleting antibiotic-based applications, phage redesign is self-fortifying. In order to address the increasing number of epidemic MRSA strains, this model could prove to be a perfect platform for developing trustworthy control and treatment strategies. Additionally, it is believed to be an infinite supply of anti-MRSA lytic phages from which several permanent phage lysins can be isolated and refined.

Scopus Crossref
View Publication
Publication Date
Wed Dec 30 2020
Journal Name
Al-kindy College Medical Journal
Effectiveness of conventional phototherapy, intensive phototherapy and exchange transfusion in treating neonatal jaundice at Fatima Al-Zahra Hospital for maternity and children in Baghdad
...Show More Authors

Background: Neonatal hyperbilirubinemia is a common disease in neonates especially in early days of birth that requires a good and successful treatment for reducing the severity and its complications that can produce important and irreversible effects.

Objectives: To evaluate the effectiveness of conventional phototherapy, intensive phototherapy and exchange transfusion on outcomes of neonatal jaundice at Fatima Al-Zahra Hospital for maternity and child care in Baghdad.

Patients & Methods: A retrospective study was carried out using medical records of neonates with diagnosis of unconjugated jaundice, admitted in the septic neonatal care unit of Fatima Al-Zahra hospital over 6 months period b

... Show More
View Publication Preview PDF
Crossref (3)
Crossref
Publication Date
Wed Jan 01 2020
Journal Name
Plant Archive
Isolation of candida spp. From cancer patients who suffered oral candidiasis due to immunodeficiency
...Show More Authors

solation of candida spp. From cancer patients who suffered oral candidiasis due to immunodeficiency

Scopus (2)
Scopus
Publication Date
Thu Jan 13 2022
Journal Name
Iraqi Journal Of Science
A Comparative Study of Amoxicillin Sensitivity Against Escherichia coli Isolates Isolated from Urinary Tract Infections
...Show More Authors

The purpose of this study was to investigate the bacterial etiology of urinary tract infections microbiologic properties of Escherichia coli isolated from urinary tract infection patients against nine amoxicillin antibiotic. E.coli isolates were collected from patients samples suffering from urinary tract infection, based on biochemical tests of Epi 20 system .Nine Amoxicillin antibiotics were selected (some vials and other are capsules) which manufactured in different countries were bought from local pharmacies in Baghdad, for the purpose of knowing the inhibitory activity of these antibiotics on E.coli one of the main microorganisms to cause urinary tract infection, the antibiotics were prepared in a concentration of 100mg/ml and their

... Show More
View Publication Preview PDF
Publication Date
Sun Jun 27 2021
Journal Name
Iraqi National Journal Of Nursing Specialties
Prevalence of Smoking among Health Workers and Effectiveness of Instructional Booklet concerning Risks of Smoking on Health Workers′ Knowledge in Baghdad Teaching Hospital
...Show More Authors

Objective(s): The present study aims at assessing the prevalence of smoking among health workers in Baghdad Teaching Hospital and to find out the effectiveness of instructional booklet concerning risk of smoking on health workers′ knowledge in hospital.

Methodology: A pre-experimental design study was conducted from 1st of October 2019 to 17th of March 2020. A non-probability sample consists of (500) participant (343 non-smokers and 157 smokers) from (1500) health workers in Baghdad Teaching Hospital, they included the physicians, nurses, pharmacists, physiotherapists, Laboratory Technicians, Medical Assistants. A questionnaire is constructed to evaluate the effectiveness of instructional booklet on health workers’ knowledge

... Show More
View Publication Preview PDF
Publication Date
Fri Jun 16 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Optimization of Bis-anthraquinones Production from Endophytic Fungi Diaporthe sp. GNBP-10
...Show More Authors

Bis-anthraquinones with a unique molecular backbone, (+)-2,2’-epicytoskyrin A (epi) and (+)-1,1′-bislunatin (bis), was produced by endophytic fungi Diaporthe sp GNBP-10 associated with Gambir plant (Uncaria gambier). Epi and bis possess robust antimicrobial activity toward various pathogens. This study focus on knowing the optimum condition of epi and bis production from Diaporthe sp GNBP-10. A series of culture media with various nutrient compositions was investigated in epi and bis production. The content of epi and bis was determined by measuring the area under the curve from TLC-densitometric (scanner) experiment. The linear regression analysis was then applied to obtain the results. The optimi

... Show More
View Publication Preview PDF
Scopus Crossref
Publication Date
Wed Jul 20 2022
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Optimization of process parameters for biodiesel production from three indigenous vegetable oils
...Show More Authors

Optimization procedures using a variety of input parameters have gotten a lot of attention, but using three non-edible seed oils of Jatropha (Jatropha curcas), Sesame (Sesamum indicum), and Sweet Almond (Prunusamygdalus dulcis) has a few advantages, including availability and non-food competitiveness. Optimizing a two-stage trans-esterification process using a sodium hydroxide-based catalyst at a fixed catalyst (1.0wt %) and temperature (60 oC) while varying molar ratio (1:3, 1:6, 1:12),  time (20–60 min), and mixing speed (500–1000 rpm), to produce optimal responses of yields were studied using response surface methodology (RSM). The optimization solution of molar ratio (1:3), time (40.9 min.),

... Show More
View Publication Preview PDF
Crossref
Publication Date
Fri Jan 01 2021
Journal Name
International Journal Of Drug Delivery Technology
Phytochemical screening and isolation of new compounds
...Show More Authors

Scopus (4)
Scopus
Publication Date
Wed Jan 01 2020
Journal Name
Annals Of Tropical Medicine And Public Health
Isolation and identification of fungi from fish feedstuff of cyprinus carpio and detection of aflatoxin b1 and ochratoxin a using ELISA technique
...Show More Authors

This study was conducted at the College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad. The aim of this study was to isolate and diagnose fungi from fish feedstuff samples, and also detection of aflatoxin B1 and ochratoxin A in fish muscles and feedstuffs. Randomly, the samples were collected from some fish farms from Baghdad, Babil, Wasit, Anbar, and Salah al-Din provinces. This study included the collection of 35 feedstuff samples and 70 fish muscle samples, and each of the two fish samples fed on one sample of the feedstuff. The results showed the presence of several genera of different fungi including Aspergillus spp, Mucor spp., Penicillium spp., Yeast spp., Fusarium spp., Rhizopus spp., Scopiolariopsis spp., Ep

... Show More
View Publication
Crossref (1)
Crossref
Publication Date
Thu Jan 13 2022
Journal Name
Iraqi Journal Of Science
Performance of VITEK 2 in the routine identification of bacteria from positive blood cultures in Sulaimani pediatrics’ hospital
...Show More Authors

Sepsis is a major cause of death worldwide among hospitalized patients, however, an accurate and advanced identification method is associated with improved sepsis patient survival. This Retrospective study carried out in Sulaimani pediatric teaching hospital from January 2014 to July 2015 and aimed to compare the effectiveness of VITEK 2 system with traditional manual procedures for identification of pathogenic bacteria in patients with a serious disease like sepsis. The positive blood cultures were divided into two groups; 138 positive cultures identified by conventional manual methods and 104 positive cultures identified by automated VITEK 2 system. The results showed that VITEK 2 system identified 16 genera and 30 species whereas only

... Show More
View Publication Preview PDF
Publication Date
Fri Jul 01 2022
Journal Name
Iraqi Journal Of Science
Extraction and Partial Purification of Lipopolysaccharide from Clinical Proteus mirabilis Isolate and Compared with Standard Bacteria
...Show More Authors

Ninety five samples were collected from different samples (urine, ear and wounds swaps), from hospitals in Baghdad city. The results of cultural, microscopic , biochemical tests indicated that in urine samples E.coli have high occurrence frequency 19 (47.5%) followed by Proteus mirabilis 18(45%) and Klebsiella species 1 (2.5%), while in wounds samples each of Pseudomonas spp. and Proteus mirabilis 10 (25%) , then followed by E.coli with 5 (12.5%) and Klebsiella species 3 (7.5%). Ear swaps samples revealed that Pseudomonas aeruginosa 7 (46%) was the major bacterium followed by Proteus mirabilis 4(26.6).Sensitivity test against eleven antimicrobial agents was done for all of the Proteus mirabilis isolates (32 isolates). The results display

... Show More
View Publication Preview PDF