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Isolation, optimization, and redesigning of phages of methicillin-resistant Staphylococcus aureus from clinical hospital isolates in Baghdad
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Background: A global health concern is methicillin-resistant Staphylococcus aureus (MRSA). The use of bacteriophages is one of the many novel control strategies against MRSA that are frequently sought. However, it is quite challenging to isolate enough lytic anti-MRSA phages. In order to extract, optimize, and remodel anti-MRSA phages, this study sought novel approaches.

Methods: Two ATCC MRSA strains and nine clinical MRSA isolates were used to isolate wild anti-MRSA phages from hospital settings, dirt, and sewage. The wild phages were optimized using plaque-based biokinetic techniques. Using chemicals that weakened bacterial cell walls, the resulting highly lytic and specific anti-MRSA phages were subjected to unique physicochemical phage redesign processes. This allowed the phages to enter host bacteria and acquire the specificity of the new host. Three different protocols were tested using combinations of Tween 20, lysozyme, and nisin A.

Results: Nisin A and lysozyme protocols at different rates were found to be successful in producing newly redesigned, transiently stable, anti-MRSA phages.

Conclusion: Unlike self-depleting antibiotic-based applications, phage redesign is self-fortifying. In order to address the increasing number of epidemic MRSA strains, this model could prove to be a perfect platform for developing trustworthy control and treatment strategies. Additionally, it is believed to be an infinite supply of anti-MRSA lytic phages from which several permanent phage lysins can be isolated and refined.

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Publication Date
Sun Mar 02 2014
Journal Name
Baghdad Science Journal
Inhibition activity of Saccharomyces boulardii and Lactobacillus acidophilus against pathogenic E.coli isolates from Recurrent Urinary Tract Infection in women In Vitro
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The aims of study is to detect the inhibitory effect of Saccharomyces boulardii and Lactobacillus acidophilus on Escherichia coli that has been isolated from recurrent urinary tract infection in women. The sensitivity of E.coli isolates to antibiotics had been studied and the most resistant E.coli isolate to antibiotics had been studied .The cup assay was used on nutrient agar and Muller-Hinton agar to detect the inhibitory activity for each S.boulardii yeast grown on YEGP media and L.acidophilus grown on MRS media in which the result showed a high inhibition activity for each of them .Also in this study the adhesion property of E.coli had been evaluated in the presence of S.boulardii at concentration of 1×109 and L.acidophilus at conc

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Publication Date
Thu Jun 01 2017
Journal Name
Journal Of Economics And Administrative Sciences
Robust Queues Models and its Role in Improving Performance in the City of Medicine / Baghdad Teaching Hospital / Clinic Internal Medicine Advisory
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The purpose of this research is to a treatment the impact of Views outliers to the estimators of a distributed arrival and service to the theory of queues and estimate the distribution parameters depending on the robust estimators, and when he was outliers greatest impact in the process of estimating the both distributions mentioned parameters, it was necessary to use way to test that does these data contain abnormal values ​​or not? it was used the method ( Tukey ) for this purpose and is of the most popular ways to discover the outliers , it shows that there are views abnormal (outliers ) in the estimators of each of the distributional arrival and service, which have a significant impact on the calculation of these estimato

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Publication Date
Fri Aug 01 2014
Journal Name
Journal Of Economics And Administrative Sciences
Constructing Quality House To Improve Health Services – an applied research in Educational Baghdad Hospital
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     Quality function deployment tool is trying to improve health services through this study that will be applied in health sector environment , and be based on applying quality function deployment tool (QFD) TO preferable evaluation of main patients requirements in order to determine the technical requirements that need most attention across improving and developing health services .                

   Main requirements are determined to patients lying in the hospital (under research) which is (educational Baghdad \ medicine city office) in Baghdad, and other technical requirements through pers

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Publication Date
Sat Jul 01 2023
Journal Name
Biomedicine
Antibacterial action of AgNPs produced from different isolates of Gram positive and Gram-negative bacteria on biofilm of Klebsiella pneumoniae isolated from RTI
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Introduction and Aim: Klebsiella pneumoniae is a Gram-negative bacterium responsible for a wide range of infections, including respiratory tract infections (RTIs). This research was aimed to study the antibacterial and anti-biofilm effect of AgNPs produced by Gram positive and negative bacteria on RTIs associated with K. pneumoniae.   Materials and Methods: The biofilm formation of K.  pneumoniae was determined by tube method qualitatively from select bacterial species characterized by UV-Visible spectroscopy. The antibacterial susceptibility of the bacteria AgNPs was tested for their antibacterial and antibiofilm activity on a clinical isolate of K. pneumoniae.   Results: K. pneumoniae isolated from RTIs were strong biofilm prod

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Publication Date
Thu Jun 01 2023
Journal Name
Biomedicine
Antibacterial action of AgNPs produced from different isolates of Gram positive and Gram-negative bacteria on biofilm of Klebsiella pneumoniae isolated from RT
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Introduction and Aim: Klebsiella pneumoniae is a Gram-negative bacterium responsible for a wide range of infections, including respiratory tract infections (RTIs). This research was aimed to study the antibacterial and antibiofilm effect of AgNPs produced by Gram positive and negative bacteria on RTIs associated with K. pneumoniae. Materials and Methods: The biofilm formation of K. pneumoniae was determined by tube method qualitatively from select bacterial species characterized by UV-Visible spectroscopy. The antibacterial susceptibility of the bacteria AgNPs was tested for their antibacterial and antibiofilm activity on a clinical isolate of K. pneumoniae. Results: K. pneumoniae isolated from RTIs were strong biofilm producers. The ant

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Publication Date
Sun Mar 02 2008
Journal Name
Baghdad Science Journal
Colonization of Staphylococcus xylosus in the kidneys and bladder of mice
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Two isolates of Staphylococcus xylosus (urease producer and non urease producer) were injected in mice at a dose of 2 × 108 colony-forming units (CFU) intraurethrally. Results showed that both isolates were able to colonize kidney and bladder of the injected mice, regardless of their urease production. Moreover, there were insignificant differences between the two groups. These results emphasized the pathogenicity of this bacteria in UTI.

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Publication Date
Thu Mar 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
DNA Sequences of LasB Gene in Pseudomonas aeruginosa Isolated from Some Clinical Cases
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 Out of 120 isolates from different clinical cases, only 75 were found and confirmed that they belong to the Pseudomonas aeruginosa bacteria. The result revealed that the LasB virulent gene was present in 63 isolates with 63% percentage. The gel electrophoresis showed that the molecular weight of LasB gene was 300 bp. DNA sequences of LasB gene was done, and the results showed the presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotides sequence with the original sequence of amino acids revealed that there are no effects of gene mutations on translation of the product protein. 

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Publication Date
Mon Apr 23 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Antimicrobial susceptibility of Enterococcus spp. Isolated from different clinical sources in Kirkuk provency
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        One of the most important problems confronts hospitals is the strains emergence  of Enterococcus spp. with multiple resistance to antibiotics, which propel researchers to modify or produce new antibiotics or combination between two antibiotics so that to be more effective against Enterococcus . This study was aimed to susceptibility some of local Enterococcus spp. Isolates with of 21 antibiotic using  disc diffusion method. The results showed absolute resistant 100% toward (Cephalexin , Gentamycin , Amikacin ,Erythromycin and Nalidixic acid), while showed a high sensitivity toward (Vancomycin and Impenem ) at percentage of 92.3% for each . Also highl

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Publication Date
Thu Sep 12 2019
Journal Name
Al-kindy College Medical Journal
The outcome of newborn admitted in neonatal care unit, center teaching hospital of pediatric , Baghdad Al-krakh, 2015
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Background: Neonatal period is a very vulnerable period of life due to many problems, In spite of advances in perinatal and neonatal care still, the mortality rate of neonate high especially in developing country The World Health Organization estimates that globally four million neonatal deaths per year, Developing countries account for around 99% of the neonatal mortality in the world, In Iraq. Neonatal mortality rate about 19 per 1000 live births which represent 56% of child death below 5 years age in 2012. The hospital in the study represents the larger pediatric hospital in Iraq. It contains 400 children's beds and 24 neonatal incubators.

Aims of the study: are to determine the institutional

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Publication Date
Sat Oct 28 2023
Journal Name
Baghdad Science Journal
Isolation and Identification of Polyethylene Terephthalate Degrading Bacteria from Shatt Al-Arab and Sewage Water of Basrah City
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Biodegradation is utilizing microorganisms to degrade materials into products that are safe for the
environment, such as carbon dioxide, water, and biomass. The current study aims to isolate and characterize
bacteria with polyethylene terephthalate (PET) degradation ability isolated from Shatt al-Arab water and
sewage from Basra, the bacteria were identified as Klebsiella pneumonia. According to the findings, the
isolates showed a highly significant difference in degradation of PET (24% during 7 days) and the percent of
degradation increased to 46% at 4 weeks compared to the control. The study also involved determining the
optimum temperature of K. pneumonia growth, which was 37°C, while the preferred

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