Swarming is one of the most important virulence factors used by bacteria to invade new sites. This study aimed to test the effects of gentamicin on swarming motility of Pseudomonas aeruginosa, both phenotypically and molecularly. The present results revealed that 11/25 isolates had gentamicin MIC of 1024 µg/ml. However, gentamicin at sub-minimal inhibitory concentration significantly (P< 0.05) reduced the diameter of swarming in all P. aeruginosa isolates. Noticeably the mean and median swarming diameter before treatment with gentamicin 5.557 and 5.816 cm respectively had significantly (P < 0.001) reduced to 0.871 and 0.766 cm respectively. At the molecular level, amrZ (a global regulator of multiple genes) and rhl (responsible for rhamnolipid production) were variably affected by gentamicin. More likely it can be concluded that amrZ and rhl are not fully responsible for swarming in P. aeruginosa isolates.
Vancomycin Staphylococcus aureus (VRSA) is a strain belonging to S. aureus that is considered the main cause of bacterial skin and soft tissue infections. It has acquired resistance to vancomycin and represents a therapeutic challenge. The current study aimed to compare the possible therapeutic effects of VRSA bacteriocin (VRSAcin) on the treatment of skin infection in mice compared with an antibiotic (linezolid). The results showed that from fifty swabs obtained from human skin wounds, only 30 samples were identified as Staphylococcus spp., and 20 samples of them were identified as VRSA strains. One isolate was selected for VRSAcin extraction depending on its antibiotic resistance using an
This study included an analysis of three stations (Al Dora, Al Za'franiya, and Arab Ejbur) chosen to study the Physiochemical and microorganism (Fungi and Bacteria) loud of the Tigris River in the southern section of Baghdad city. The result of this research shows that the highest temperature recorded in summer in Al Za'franiya was 37Co, while the lowest temperature recorded in winter in Al Dora was 9Co. and the value of pH recorded the highest in summer it was 7.9 in Arab Ejbur, and the lowest value was in winter 7.1 in Al Dora regions, While Total Organic Carbon (TOC) shows the highest values found in the summer was 6.7 Mg L-1in Al Za'franiya Samples, and the lowest values were 2.0 Mg L-1in Arab Ejbur during the winter. The more f
... Show MoreThree types of medical commercial creams Silvazine, Cinolon Tar and Hydroquinon Domina were incorporated in this study. The medical creams were taken directly and placed uniformly on the glass slide. Each type of pharmaceutical was weighed at 1 mg and dispersed on an area of 1x1 cm. This process ensures same thickness for all samples. The creams were analyzed by using double-beam UV/visible spectrophotometer Metertech SP8001. The absorption spectrum for each of samples was measured against wavelength range of 300–700 nm.
Took apple branches Genuine and Architecture tissue cultured in vitro 3 cm long and planted in the middle of food MS that contains different concentrations of inorganic salts and of Mntmat free growth and incubated Transplanter to study their effect on rooting Aalavra
Background: Refractory/relapsed acute leukemia has always been a challenging problem for hematologist. Over the past decade emphasis has been made in the development of regimens containing fludarabine, combined with cytosine arabinoside for the treatment of refractory/relapsed acute leukemias. The aim of this study is to evaluate the efficacy and toxicity of the combination of fludarabine, high dose cytarabine, and granulocyte colony stimulating factor in refractory relapsed cases of acute leukaemia,
Methods: a prospective study is being conducted at the national center of hematology and hematology unit /Baghdad teaching hospital from July 2008 to July 2010.Twenty Patients with refractory/relapsed acute leukemia were treated with flud
A total of 50 wells water samples were collected from 10 wells in Abu Ghraib site/ Baghdad for detection of coliform, fecal coliformand and pseudomonas aeruginosa bacteria, and the pollution of toxic ions (No3-, B+3, pb+2 and Cd+2 ) in wells water. Results showed microbial pollution by coliform, fecal Coliform bacteria in wells water when using presumptive, confirmed and complete tests, P. aeruginosa bacterium was isolated and identificated on Cetrimide Agar media, estimated the Most Probable Number (MPN) of coliforms and P. aeruginosa, results showed difference in mean of (MPN) of wells water. Most of toxic ions concentrations were low comparing with the recommended hygienic standards from the World Health Organization (WHO). Wells wate
... Show MoreIn this research , the structural and optical properties of pure of cadmium oxide, pure (CdO) were studided thin films in a thermal evaporation in a vacuum depositing metal cadmium pure rules of the glass at room temperature (300K) and thickness (300 ± 20nm) and the time of deposition (1.25sec) was oxidation of thin films cadmium (Cd) record temperature (673k) for a period of one hour to the presence of air optical energy gap for direct electronic transitions were calculated (permitted) as a function of absorption coefficient and permeability and reversibility by recording the spectrum absorbance and permeability of the membrane the record
... Show MoreThe Imam Ibn al-Mundhir is likely in Shafi'i school. He has outlined some issues and disagreed with his doctrine. And that the vocabulary on which he relied on the weighting is correct and correct and I choose which I say. One of the most likely issues is the delay of Isha prayer to the darkness. And other issues simplified in the folds of research and study.
This study was designed to stand on the most important causes of contamination in sesarean section in women by the aerobic bacteria species the bacteria isolates were diagnosed in the Maternity and Children Hospital in the Qadsiya province and in the special clinics. Sixty five persent of women having cesarean operation were contaminated with different bacteria in different ratios. Staphylococcus aureus showed the higher percentage (62.4) , Pseudomonas.aerogenosa 18% , E.coli 9.5% and Proteus.spp (6,4%).While Streptococcus pyogens showed lowest percentage 3.6% . The results of antibiotic sensitivity test for all bacteria isolates showed Chloramphenicol and Ami
... Show MoreThis study which was carried out from Jan /2010 to Jan/2011,evolution of the efficiency
of treatment unit of al-Ameen factory , a subsidiary of Vegetable Oils General Company in
al- Za
,
faraniya /Sa
,
idea district /South of Baghdad ,via examing the waters coming out of
treatment unit and the role of this unit in improving waters quality , especially in physical and
chemical characteristics to be main factors in studying water's quality, such as temperature
C°,pH,Ec,DO,BOD5,NO3,TDS,PO4
-3
.
The results showed that the characteristics of treated treated water excepte of the Ec
factor were within the acceptable limits,in spite of the high concentration entering the unit .
This confirms t