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Synergistic Activity of Indolicidin and Tigecycline Against Multidrug-Resistant Acinetobacter Baumannii Clinical Isolates
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Background/Aims: Acinetobacter baumannii is a ubiquitous opportunistic microorganism associated with high morbidity and mortality Particularly among burn patients and immunocompromised individuals. This study aimed to assess the time-dependent antimicrobial effectiveness of indolicidin in combination with tigecycline against multidrug-resistant (MDR) A. baumannii isolates from patients with wound infections. Methods: The antimicrobial synergy between indolicidin and tigecycline was evaluated using the checkerboard broth microdilution method And anti-biofilm activity was assessed using the crystal violet assay. Ten isolates resistant to multiple antibiotics were identified and confirmed using the API 20-NE system. Results: Fractional inhibitory concentration (FIC) values ranged from 0.97 to 250 µg/ml. The results demonstrated that the inhibitory activity of indolicidin and tigecycline was significantly enhanced when combined With FIC values between 0.046 and 0.312 µg/ml. The MIC of indolicidin for isolate A4 decreased from 62.5 to 0.97 µg/ml • While the MIC of tigecycline decreased from 500 to 15.6 µg/ml • Indicating a strong synergistic effect. Additionally The combination therapy led to a 78% reduction in biofilm formation. Conclusion: This study suggests a novel combination therapy for treating wound infections caused by MDR A. baumannii • Demonstrating significant efficacy in both bacterial inhibition and biofilm reduction.

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Publication Date
Wed Jun 10 2026
Journal Name
Adolescência E Saúde
Assessment Of The Effect Of 3-Caffeoyl Quinic Acid On Biofilm Formation By Kocuria Kristinae Clinical Isolates From Ear Infections
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Background: Kocuria kristinae (earlier known as Rothia kristinae) is a relatively new opportunistic pathogen responsible for ear infections. The ability of this organism to form biofilms increases its resistance to therapy and promotes the development of antimicrobial resistance, necessitating new treatment methods. Objective: To assess the effect of 3-caffeoylquinic acid on K. kristinae isolated from patients with ear infections. Methods: Isolation of five clinical strains was done by conventional microbiological procedures. The ability of K. kristinae to form biofilms was determined by the crystal violet method (absorbance at OD₅₇₀) before and after 3-caffeoylquinic acid exposure; statistical data analysis was done using a

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Publication Date
Sat Mar 01 2025
Journal Name
Microbial Biosystems
Distribution of cytotoxic necrotizing factor type 1 in clinical isolates of Escherichia coli isolated from urinary tract infections in Iraq
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Uropathogenic Escherichia coli is the main cause of urinary tract infections, the ability of this bacteria to cause urinary tract infections is related to a variety of virulence factors that enhance colonization and evade the immune response, one of these virulence factors is cytotoxic necrotizing factor 1 toxin which converts the glutamine residue to glutamic acid to activated GTPase Rho family. The study was meant to find out the prevalence rate of the cnf1 gene in Uropathogenic Escherichia coli isolated from Iraqi patients. Conventional laboratory methods were used for primary bacterial identification and molecular methods were used to confirm bacterial identity and gene detection. Escherichia coli was identified in 89/165 (53.93%) of th

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Publication Date
Fri Jul 18 2025
Journal Name
Microbes And Infectious Diseases
Molecular discrimination for the most pathogenic serotypes of clinical isolates of Klebsiella pneumoniae using cps locus encoded to capsular antigens.
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Publication Date
Wed Apr 29 2020
Journal Name
Indian Journal Of Forensic Medicine & Toxicology
Scolicidal Activity of Zirconium Oxide (ZrO2) nanoparticles Against Protoscolices of Hydatid Cysts
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Hydatidosis is a sickness that affects human and farm animals. This disease is deemed as a public health problem in different regions of the world until nowadays. Surgical overlaps is the best way to treat the disease, while the risk of surgery lies in the possibility of cyst rupture and leakage of protoscolices and the recurrence of infection again, this prompted researchers to use scolicidal agents before surgery such as ethanol, plant extracts, to reduce parasite spread and recurrence of infection, recently researchers have been using nanoparticles as a scolicidal agent, like gold nanoparticles, silver nanoparticles, selenium nanoparticles, and others. This research aims to evaluate the fatal effect of zirconium oxide (ZrO2) nano

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Publication Date
Sun Jul 29 2018
Journal Name
Iraqi Journal Of Science
Incidence of Ciprofloxacin-Resistant of Methicillin Resistant Staphylococcus aureus isolated from Iraqi patients
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The resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.

Publication Date
Sat Jul 15 2017
Journal Name
Iraqi Journal Of Science
Antibacterial activity of Rosmarinus officinalis and Dodonaea viscosa leaves extracts against Escherichia coli and Staphylococcus aureus
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Publication Date
Wed Dec 06 2023
Journal Name
Indonesian Journal Of Chemistry
Synthesis, Characterization and Biological Activity of New Oleander Complexes against Bacteria Found in Polluted Water
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Natural polymers are often non-toxic, biodegradable, biocompatible, and safe. A novel ligand was synthesized as a natural polymer using chitosan and oleander plant extract [(2R,3S,4R,5S)-5-(acetoxyamino)-4-hydroxy-3,6-dimethoxytetrahydro-2H-pyran-2-yl) methyl (16R)-3-(((2S,4S,5R)-4-methoxy-2,5-dimethyltetrahydro-2H-pyran-2-yl)oxy-10,13,16-trimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl) hexadecahydro-14H-cyclopenta [a] phenanthren-14-yl) phthalate] (Chitosan-Ph-Oleander). This ligand and its complexes with several metals (Cr+3, Mn+2, Fe+3, Ni+2, Cu+2, Zn+2) were characterized using FTIR, UV-visible and 1H-NMR spectroscopy, as well as by molar conductivity, magnetic moment, and TGA analysis. The biological activity for the prepared polymer

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Publication Date
Fri Feb 28 2020
Journal Name
Iraqi Journal Of Agricultural Sciences
INSECTICIDAL ACTIVITY OF EUCALYPTUS SP. VOLATILE OIL AGAINST BACKSWIMMER INSECT ANISOPS SARDEA
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The objective of this study was to evaluate a natural bio-insecticide manufacturing from Eucalyptus sp. volatile oil. The use of Eucalyptus sp. against the Backswimmer insect Anisops sardea Herrich-Schaeffer, 1849 predatory of larvae of common carp fish, Cyprinus carpio L., in artificial closed ponds in Babylon province represented a new idea in Iraq. The volatile oil of the Eucalyptus sp. was extracted by hot water method using the Clevenger, three concentrations of 250000, 450000 and 650000 ppm with benzyl benzoate as a stabilizer were used, which has a boiling point of 324OC (slow evaporation) at field experiment.The results of field and laboratory experiments of the extracted volatile oil in different concentrations, showed that

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Publication Date
Tue Jan 01 2019
Journal Name
Indian Journal Of Public Health Research & Development
Antibacterial Activity of <i>Lactobacillus</i> spp. Against <i>Listeria monocytogenes</i>
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Publication Date
Wed Jun 28 2023
Journal Name
The Iraqi Journal Of Veterinary Medicine
Antibacterial Activity of Lactiplantibacillus plantarum‎ from Dairy Products ‎Against Some ‎Foodborne Bacteria
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Lactiplantibacillus plantarum‎, one of lactic acid bacteria (LAB), is found in various foods, ‎‎including dairy products, meat, and vegetables, and most of these bacteria offer beneficial ‎‎effects to humans and animals as potential probiotics with broad-spectrum antimicrobial ‎‎activities. The aim of this study was evaluating the antibacterial efficacy of L. ‎plantarum‎ ‎against ‎some foodborne bacteria isolated from dairy products. This research involved 34 dairy ‎products, ‎including local and imported milk, cheese, and yogurt sold locally in Baghdad ‎province, Iraq, during May ‎‎2022. For the isolation of L. ‎plantarum‎, a special medium called ‎MRS (de Man Rogosa and ‎Sharpe) was applied. Colonie

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