The ability of microorganisms to attach to living and non-living surfaces and create a biofilm is the cause of numerous long-lasting illnesses, as well as their strong resistance to drugs. Bacterial biofilms consist of intricate assemblies of immobile bacteria. These are located in an extracellular matrix and adhere to various surfaces for a long period. The present study evaluated the antibacterial effectiveness of Plantago major extract against Staphylococcus aureus biofilm. The specimens analyzed in this investigation were skin infections of clinical origin. The current study was not previously studied, particularly in terms of S. aureus biofilm breakdown and inhibition. The disc diffusion method was used to test the antimicrobial activity of extracts on planktonic forms. Several antibiotic control tests were conducted utilizing several commercial disks, The sizes of the inhibitory zones were measured in millimeters and normalized. The anti-biofilm effects were evaluated using the microtiter plate technique. Out of 120 clinical samples, only 46 (38.3 %) had positive S. aureus isolates, whereas 74 (61.6%) were negative. The current study demonstrated that ethanolic P. major leaf extract has antibacterial effects on the development of S. aureus isolates with zones of inhibition ranging in size from 9 to 22 mm. Simultaneously, Antibiotic susceptibility tests showed that all isolates were resistant to erythromycin and clindamycin at an 80 % level. Cefoxitin and chloramphenicol resistance was found at 72 %, and 77 %, respectively. significance and impact of study: this research shown that Extracts from Plantago major can be employed as antibacterial agents against S. aureus, and also anti_staphyloccocus biofilm forms. Keywords. Plantago major, staphylococcus aureus, Biofilm, Antimicrobial activities, Microtiter Plate.
The resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.
The resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.
(4)
(1)
Background: Insertion sequence is a short DNA sequence encode for proteins implicated in the transposition activity. Transposase catalyzes the enzymatic reaction allowing the insertion sequence to +9*lo2 move. ;qqa;.
Objective: To study the sequencing of transposase gene, tnp, IS1216V of S. aureus isolated from food and then compared with that documented in National Center for Biotechnology Information (NCBI).
Methods: Food samples of animal
... Show More
Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to form biofilm is considered as one of the most important virulence factors of coagulase negative staphylococci. There is only limited knowledge of the nature of S. haemolyticus biofilms. This study was aimed at evaluating the ability of S. haemolyticus strains to produce biofilm in the presence of copper oxide nanoparticles (CuONPs). The biological synthesis of nanoparticles is an environmentally friendly approach for large-scale production of nanoparticles. Copper oxide nanoparticles were produced in the current study from the S. haemolyticus viable cell filtrate. UV-visible (UV-Vis) spectroscopy, X-ray diffra
... Show More
This study was done to investigate the antibacterial effect of the three types of Lawsonia inermis linn (henna) leaf extracts (water, methanol and chloroform) in different concentrations (40, 80, 120) mg/ml against four strains of bacteria (Staphyllococus aureus, Bacillus subtilis, Pseudomonos aerogenosa and Eschorichia coli) in vitro using ager well diffusion method. Water extract showed the highest antibacterial activity, followed by methanol extract, while the chloroform extract showed the lowest activity. The maximum inhibition zone of water extract was observed against , Pseudomonos aerogenosa (25mm) in the concentration (120) mg/ml, while the minimum zone of inhibition (9mm) was in Bacillus subtilis in the same concent
... Show MoreAbstract A total of 207 specimens were collected from different sources including patients, health care staff and hospital environment in Ibb city, Yemen. The study used the bacteriocin produced from active producer strains in typing of Staphylococcus aureus. Depending on the morphological, cultural and biochemical characteristics, 54 (26.09%) isolates of Staphylococcus aureus were identified. An antibiotic sensitivity test was done for the bacterial isolates, and the results showed that there were multiple resistant antibiotics. The Staphylococcin production of these isolates has been detected by using wells assay. Fifty one isolates were Staphylococcin producer. Four isolates (staph19, staph25, staph28 and staph43) were chosen as go
... Show More
(1)
Crade extract of dried fruit piper nigrum was made by useing sexhdet for three hours.The dried material was extracted with 95% ethanol and water as solvent. A test was made to the extract effect in certain concentration to 32 Gram of negative bacterial isolates , collected from patient admitted to Ibn-AL-balade hospital . Ethanolic extract showed antibacterial activity against bacterial isolates and water extract reveled effectivnes.Such isolates showed highly sensitive to Norfacin and Impinene and less sensitve to Amoxicillin .
This study was conducted to investigate the presence of Staphylococcus aureus in the red and white meat available in local markets. They were selected ten samples of red and white meat randomly (Iraq, Saudi Arabia, Turkey, and Brazil) from different markets in Baghdad, and the results of reading the nutrition facts of media indication card showed that all models confirm to the Iraqi standard quality in terms of scanning all data of the media indication card, except for the birds of Bayader, where the date of expire & production date of the product was not mentioned. Also, the results of the study showed that there is no Staphylococcus aureus in local red and white meat as well as imported.