Clinical index is needed to predict the outcome of pregnancy after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) for infertile patients. Growth differentiation factor-8 (GDF-8), also known as myostatin, is one of transforming growth factor-â superfamily localized in antral follicles in normal and PCOS ovaries but its function in female reproductive system is still unknown. Aim of the study is to assess the correlation between levels of GDF8 in follicular fluid (FF) with outcomes of in vitro fertilization (IVF/ICSI) in women with and without PCOS. A prospective case control study was performed enrolling (40) patients with PCOS and (40) non-PCOS women (male infertility) undergoing IVF/ICSI. The collection of follicular fluid was at the day of oocyte pick up. Sandwich enzyme-linked immunosorbent assay (ELISA) kit was used to measure the levels of FF. GDF-8. A significant higher GDF8 level was found in PCOS group compared to non-PCOS group. Also, significant higher antral follicle count (AFC) in PCOS group in comparison tonon-PCOS group. There were no significant differences between the two groups in the mean of follicle diameter, endometrium thickness, aspirated oocytes, metaphase II (M II) oocyte, fertilized oocytes, embryo at 2pro nucleus (2PN), transferred embryo, grade1 (G1) embryo, maturity rate, cleavage rate, fertilization rate and pregnancy outcomes. There was a significant positive correlation between GDF8 and G1 embryo in non-PCOS group. In non-PCOS group, mean GDF8 level was significantly higher in pregnant group than nonpregnant group. In PCOS group, mean GDF8 level was significantly.
New Schiff base and their Mn(II),Co(II),Ni(II), Cu(II) and Hg(II) complexes formed by the condensation of O-phathaldehyde and ethylene diamine (2:1) to give ligand (L1) in the first step ,then the ligand (L1) with 2- aminophenol (1:2) to give ligand (L2) were prepared by classic addition through microwave method . These compounds (Ligands and complexes) have been diagnosed electronic spectra, FT-IR, 1H-&13C-NMR (only ligand), magnetic susceptibility, elemental microanalysis and molar conductance measurements. Analytical values displayed that all the complexes appeared (metal: ligand) (1:1) ratio with the six chelation. All the compounds appear a high activity versus four types of bacteria suc
... Show MoreNew binuclear Mn(II), Co(II), Ni(II), Cu(II), Zn(II), and Hg(II) Complexes of N2S2 tetradentate or N4S2 hexadentate symmetric Schiff base were prepared by the condensation of butane-1,4-diylbis(2-amino ethylcarbamodithioate) with 3-acetyl pyridine. The complexes having the general formula [M2LCl4] (where L=butane-1,4-diyl bis (2-(z)-1-(pyridine-3-ylethylidene amino))ethyl carbamodithioate, M= Mn(II), Co(II), Ni(II), Cu(II), Zn(II), and Hg(II)), were prepared by the reaction of the mentioned metal salts and the ligand. The resulting binuclear complexes were characterized by molar conductance, magnetic susceptibility ,infrared and electronic spectral measurements. This study indicated that Mn(II), Ni(II) and Cu(II) complexes have octahedral g
... Show MoreBack ground: Epstein- Barr virus (EBV) is a ubiquitous in that infecting more than 90% of adult population worldwide. Recently, EBV has been linked to the development of variety of human malignancies. P53 gene is mutated in more than 50% of human cancers. Cell cycle dysregulation, measured by p53 protein expression, and latent EBV infection are important in the pathogenesis of Non-Hodgkin’s lymphomas.
Objective: To analyze the distribution and impact of concordant p53 expression and latent EBV infection on a group of B & T cell types of NHL.
Materials and Methods: Forty (40) formalin-fixed, paraffin embedded tissue blocks were obtained from lymph nodes biopsies related to patients with NHL.
In addition, biopsies of twenty
This study involves the synthesis of new azodye, derived from 2-Amino-6ethoxybenzothiazole and 4-Chloro-3,5-dimethylphenol . The characterization of dye has been described by C.H.N. The TG , IR and Visible. spectroscopic techniques .The acid-base properties were studied at different pH values . The ionization and protonation constants of dye were determined. Solvents effects were also studied at different solvents polarities . The optimum conditions of this formation of complex with Fe(III) were investigated . The analytical applications of this azodye , were studied like; using it as acid-base indicator , and for the determination of nitrite ions
In this study terpolymer resin ) p-ABBF) was prepared by condensation of (p-amino benzoic acid) and (Biuret) with formaldehyde in (1:1:3) mol ratio using hydrolic acid as a reaction medium at 130±2 ℃ .The synthesized terpolymer resin was characterized by elemental analysis , FT-IR and (1H-NMR) spectroscopy. The intrinsic viscosity was determined. The thermal stability of the terpolymer was analyzed by (TGA and DSC).The morphological feature of the (p-ABBF) terpolymer resin was studied by scanning electron microscopy (SEM).Bach equilibrium method was employed to study analytical efficiency of the terpolymer resin towards certain trivalent and divalent metal ions such as (Cu+2,Ni+2, Co+2,Zn+2,Cd+2 and Cr+3( where thes
... Show MoreThe presence of natural voids and fractures (weak zones) in subsurface gypsiferous soil and gypsum, within the University of Al-Anbar, western Iraq. It causes a harsher problem for civil engineering projects. Electrical resistivity technique is applied as an economic decipher for investigation underground weak zones. The inverse models of the Dipole-dipole and Pole-dipole arrays with aspacing of 2 m and an n-factor of 6 clearly show that the resistivity contrast between the anomalous part of the weak zone and the background. The maximum thickness and shape are well defined from 2D imaging with Dipole-dipole array, the maximum thickness ranges between 9.5 to 11.5 m. It is concluded that the 2D imaging survey is a useful technique and more
... Show MoreThe fluorescence emission of Rhodamine 6G (R6G) and Acriflavine dyes in PMMA polymer have been studied by changing the irradiation and exposure time of laser light to know the effect of this parameter. It was found that the fluorescence intensity decreases in the polymer samples doped dyes as the exposure time increases and then reaches stabilization at long times, this behavior called photobleaching, which have been shown in liquid phase less than solid phase. Using 2nd harmonic with wavelength 530 nm laser, the photobleaching effect in the two dye-doped polymers different solvent but same was studied. It was observed that photobleaching of by different solution and by using dip spin coating the photobleaching seem in liquid phase more
... Show MoreDiarrhea is an important public health problem worldwide, several causes associated with diarrhea especially in population live under poverty and unsafe water use. Different methods are available and use in diagnosis. This study was carried out to compare of various techniques for Giardia lamblia detection and study the association with E coil and Shigella in patients with diarrhea. A total of 100 children with diarrhoea were enrolled into the study, 57 were males and 43 were females, aged from 2 months -16 years were attendant to AL-Imamin AL-Kadhimin Medical City, during the period from May 2014 to February 2015. Stool samples were collected and analysed for Giardia lamblia
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