Preferred Language
Articles
/
shdijZMBVTCNdQwCHtXW
Random amplified polymorphic DNA-based polymerase chain reaction is an effective tool to examine the genotoxic effects of some food colors
...Show More Authors

A large number of natural or synthetic dyes have been removed from both national and international lists of permitted food colors because of their mutagenic or carcinogenic activity. Therefore, this study aimed to use the Random Amplified Polymorphic DNA-Based Polymerase Chain Reaction (RAPD-PCR) assay as a feasible method to evaluate the ability of some food colors as genotoxin-induced DNA damage and mutations. Lactiplantibacillus plantarum was used as a bioindicator to determine the genotoxic effects by RAPD-PCR using M13 primer after treatment with some synthetic dyes currently used as food color additives, including Sunset Yellow, Carmoisine, and Tartrazine. Besides qualitative analysis, the bioinformatic GelJ software was used for cluster analysis to compare DNA fingerprints before and after treatment. The bacteria treated with the food colors showed the presence of polymorphism represented by DNA changes in the RAPD patterns, including variation in bands intensity, disappearance of normal bands, and appearance of new bands compared with the non-treated control. The GelJ program confirmed the presence of genetic variations between the bacteria treated with different concentrations of the food dyes and the bacteria without treatment. The RAPD approach can be applied for the detection of DNA damage and mutations induced by genotoxic compounds. Furthermore, L. plantarum and M13 are suitable as in vitro screening tools for detecting of potential genotoxicity of numerous compounds.

Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Mon Oct 01 2007
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Genotoxic effects among petrol station workers.
...Show More Authors

Background: Benzene has been internationally recognized as a potent toxin, particularly for its effects on the blood forming system of the bone marrow and its association to a large number of haematological disorders.
Aims: This study aimed to assess the cytogenetic damages related to occupational exposure to benzene by calculating the mitotic index ( MI) , nuclear division cytotoxicity index ( NDCI), binucleated cells ratio and chromosomal aberrations.
Obgectives: Peripheral blood samples were collected from 30 benzene exposed workers and 10 from unexposed- controls- persons. 20 out of the exposed workers were occupationally exposed to benzene from 2 to more than 4 years. The rest of workers were exposed to benzene from one month

... Show More
View Publication Preview PDF
Crossref
Publication Date
Fri Dec 20 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Evaluation of antibacterial effect of irrigant solutions (titanium tetra fluoride, green tea, sodium hypochlorite, normal saline) using real-time quantitative – polymerase chain reaction
...Show More Authors

Background: Removal of bacteria from the pulp system by instrumentation of an infected root canal, will be significantly reduced the number of bacteria, but it is well documented that instrumentation alone can-not clean and kill all bacteria found on the root canal walls. Antibacterial irrigants are needed to kill the remaining microorganisms. The aims of this study was to assess antibacterial effect of titanium tetrafluoride (TiF4) solution and brewing green tea against root canal bacteria and to compare with sodium hypochlorite and normal saline through microbiological and molecular studies. Materials and methods: Microbiological study was carried out to determine the concentration of titanium tetrafluoride and brewing green tea at which

... Show More
View Publication Preview PDF
Publication Date
Fri Dec 20 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Quantitative detection and correlation of Epstein - Barr Virus in plasma with gingivitis and severity of chronic periodontitis by using real-time polymerase chain reaction technique
...Show More Authors

Background: The main purpose of this study is to find if there is any correlation between the level of C-reactive protein (CRP) in gingival crevicular fluid with its serum level in chronic periodontitis patients and to explore the differences between them according to the probing depth. Materials and methods: Forty seven male subjects enrolled in this study. Thirty males with chronic periodontitis considered as study group whom further subdivided according to probing depth into subgroup 1 with pocket depth ≤6mm, subgroup 2 with pocket depth >6mm. The other 17 subjects considered as controls. For all subjects, clinical examination where done for periodontal parameters plaque index (PLI), gingival index (GI), bleeding on probing (BOP),

... Show More
View Publication Preview PDF
Publication Date
Wed Jan 01 2020
Journal Name
Proceedings Of The 2020 2nd International Conference On Sustainable Manufacturing, Materials And Technologies
The impact of toxicant on the food chain ecological model
...Show More Authors

View Publication
Crossref (1)
Crossref
Publication Date
Sat Jul 31 2021
Journal Name
Iraqi Journal Of Science
Chaos in a Hybrid Food Chain Model
...Show More Authors

In this paper, chaotic and periodic dynamics in a hybrid food chain system with Holling type IV and Lotka-Volterra responses are discussed. The system is observed to be dissipative. The global stability of the equilibrium points is analyzed using Routh-Hurwitz criterion and Lyapunov direct method. Chaos phenomena is characterized by attractors and bifurcation diagram. The effect of the controlling parameter of the model is investigated theoretically and numerically.

View Publication Preview PDF
Scopus (3)
Scopus Crossref
Publication Date
Wed Oct 20 2021
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Polymer Chain Reaction (PCR): Principle and Applications
...Show More Authors

The new, standard molecular biologic system for duplicating DNA enzymatically devoid of employing a living organism, like E. coli or yeast, represents polymerases chain reaction (PCR). This technology allows an exponential intensification of a minor quantity of DNA molecule several times. Analysis can be straightforward with more DNA available.

A thermal heat cycler performs a polymerization chain reaction that involves repeated cycles of heating and cooling the reactant tubes at the desired temperature for each reaction step. A heated deck is positioned on the upper reaction tube to avoid evaporating the reaction mixture (normally volumes range from 15 to 100 l per tube), or an oil layer can be placed on a reaction mixture surfa

... Show More
View Publication Preview PDF
Crossref
Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Food Chain Borne Effect of Cadmium and Cyanide on Some Biochemical Indices in the Liver of Rats
...Show More Authors

       Diverse organismsuch as mammals and fishes exposure to noxious waste in the surroundings is a continuous routine and the active absorption and propagation of contaminants in humans is through the food chain. In order to determine the level of toxicity across the food chain,this research was structured to identify some biochemical alterations in the hepatic tissue of rats fed cadmium, cyanide and a mixture of cyanide and cadmium contaminated catfish diet. Fish were assigned into four groups and were exposed to both toxicants (cadmium and cyanide) in the single and combined states. Each toxicant was administered as cadmium chloride (CdCl2) and potassium cyanide (KCN) on a dose of 0.4 mg of the toxicant/100 ml water

... Show More
View Publication Preview PDF
Crossref (1)
Scopus Crossref
Publication Date
Fri Dec 20 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Establishment of the possible association between the presence of Helicobacter pylori in the saliva and gastric biopsy by using polymerase chain reaction technique in association with oral manifestation of peptic ulcer disease
...Show More Authors

Background: Helicobacter pylori are important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. There are several popular methods for detection of H. pylori (invasive and non-invasive methods) each having its own advantages, disadvantages, and limitations, and by using PCR technique the ability to detect H. pylori in saliva samples offers a potential for an alternative test for detection of this microorganism. Materials and methods: The study sample consists of fifty participants of both genders, who undergo Oesophageo-gastrodudenoscopy at the Gastroenterology Department of Al-Kindy Teaching Hospital Baghdad/ Iraq, during five months period from January 2014 to May 2014. They we

... Show More
View Publication Preview PDF
Publication Date
Wed May 25 2022
Journal Name
Iraqi Journal Of Science
The Dynamics of the Aquatic Food Chain System in the Contaminated Environment
...Show More Authors

     In this paper, the aquatic food chain model, consisting of Phytoplankton, Zooplankton, and Fish, in the contaminated environment is proposed and studied. Modified Leslie–Gower model with Holling type IV functional response are used to describe the growth of Fish and the food transition throughout the food chain, respectively. The toxic substance affects directly the Phytoplankton and indirectly the other species. The local stability analysis of all possible equilibrium points is done. The persistence conditions of the model are established. The basin of attraction for each point is specified using the Lyapunov function. Bifurcation analysis near the coexistence equilibrium point is investigated. Detecting the existence of chao

... Show More
View Publication Preview PDF
Scopus (3)
Crossref (1)
Scopus Crossref
Publication Date
Thu Jun 30 2022
Journal Name
Iraqi Journal Of Science
The Effect of Mutual Interaction and Harvesting on Food Chain Model
...Show More Authors

       This paper treats the interactions among four population species. The system includes one mutuality prey, one harvested prey and two predators. The four species interaction can be described as a food chain, where the first prey helps the second harvested prey. The first and the second predator attack the first and the second prey, respectively, according to Lotka-Volterra type functional responses. The model is formulated using differential equations. One equilibrium point of the model is found and analysed to reveal a threshold that will allow the coexistence of all species. All other equilibrium points of the system are located, with their local and global stability being assessed. To back up the conclusions of the mathema

... Show More
View Publication Preview PDF
Scopus (12)
Crossref (7)
Scopus Crossref