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Random amplified polymorphic DNA-based polymerase chain reaction is an effective tool to examine the genotoxic effects of some food colors
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A large number of natural or synthetic dyes have been removed from both national and international lists of permitted food colors because of their mutagenic or carcinogenic activity. Therefore, this study aimed to use the Random Amplified Polymorphic DNA-Based Polymerase Chain Reaction (RAPD-PCR) assay as a feasible method to evaluate the ability of some food colors as genotoxin-induced DNA damage and mutations. Lactiplantibacillus plantarum was used as a bioindicator to determine the genotoxic effects by RAPD-PCR using M13 primer after treatment with some synthetic dyes currently used as food color additives, including Sunset Yellow, Carmoisine, and Tartrazine. Besides qualitative analysis, the bioinformatic GelJ software was used for cluster analysis to compare DNA fingerprints before and after treatment. The bacteria treated with the food colors showed the presence of polymorphism represented by DNA changes in the RAPD patterns, including variation in bands intensity, disappearance of normal bands, and appearance of new bands compared with the non-treated control. The GelJ program confirmed the presence of genetic variations between the bacteria treated with different concentrations of the food dyes and the bacteria without treatment. The RAPD approach can be applied for the detection of DNA damage and mutations induced by genotoxic compounds. Furthermore, L. plantarum and M13 are suitable as in vitro screening tools for detecting of potential genotoxicity of numerous compounds.

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Publication Date
Tue Jun 30 2020
Journal Name
Medico-legal Update
Effects of caring children with leukemia on their mothers` psychosocial status
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Abstract Study aim: to assess the influence of care burden for children with leukemia on their mothers` psychosocial status. Methodology: A Descriptive study, conducted at two pediatric hospitals in Baghdad city. A purposive sample of (60) mothers was participated in the study after obtaining their consent form. The instrument of the study was used to assess mothers` psychosocial status in addition to their sociodemographic characteristics. The data was processed and statistically analysed by SPSS program version 23. Result: the result of the study showed mothers have (81%) in self esteem, (77%) in psychosocial distress, (80%) for social interaction, and (76%) for social isolation. There were association between mothers` psychosocial status

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Publication Date
Mon Jul 06 2020
Journal Name
International Journal Of Research In Pharmaceutical Sciences
Antibacterial and antioxidants effects of anthraquinones fraction from Clerodendron inerme leaves
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Clerodendron inerme is used to treat asthma, inflammation, Parkinson’s diseases, and other diseases.This research is aimed to determine antibacterial and antioxidant effects in the anthraquinones’ fraction of Clerodendron inerme leaves.Well-diffusion agar, minimum inhibitory concentration, and minimal bactericidal concentration were used to test the antibacterial properties of the four types of bacteria.DPPH radical scavenging assay was used to evaluate their antioxidant activity.GC-MS and FTIR analysis were used to identify anthraquinones compounds of the leaves in the plant.Anthraquinones component from the leaves of C.inerme has shown significant inhibition against pathogenic bacteria, including Pseudomonas aeruginosa. The an

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Publication Date
Sat Sep 01 2018
Journal Name
Case Studies In Thermal Engineering
An investigation of dynamic behavior of the cylindrical shells under thermal effect
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Publication Date
Sun Feb 01 2015
Journal Name
Energy Conversion And Management
A dynamic model and an experimental study for the internal air and soil temperatures in an innovative greenhouse
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An innovative greenhouse which integrates a conventional greenhouse with roof mounted solar air heaters is used in this investigation. This design reduces the solar radiation incoming to the greenhouse in summer which reduced the load and cost of greenhouse cooling and provides a means of solar heating. Experimental measurements of the internal air and internal soil sub-layer temperatures in the greenhouse, without crops, were performed in Baghdad University, Baghdad, Iraq (33.3 °N, 44.4 °E). Measurements were recorded for clear and partly cloudy winter days. A dynamic model was developed to predict the all internal temperatures of the greenhouse. This model includes soil surface heat exchange with the greenhouse air which was found to gi

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Publication Date
Mon Oct 19 2026
Journal Name
Journal Of Baghdad College Of Dentistry
Antibacterial effects of mineral trioxide aggregate and Biodentine TM after the addition of different concentrations of black seed aqueous solutions
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Background: Mineral Trioxide Aggregate (MTA) and BiodentineTM cements are new materials with numerous exciting clinical applications. Both have appreciable properties which include good physical properties and the ability to stimulate tissue regeneration as well as good antibacterial effects. The aim of this study was to investigate and compare the antibacterial effects of MTA and BiodentineTM, when they were mixed with different concentrations of aqueous solutions of Black Seed extract, against Enterococcus faecalis. Materials and methods: MTA and BiodentineTMwere prepared according to the manufacturer’s instructions. The method of Mawlood was followed to prepare the Black Seed aqueous solution. Agar diffusion method on Brain Heart

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Publication Date
Wed Jan 22 2020
Journal Name
Molecules
In Vivo and In Vitro Evaluation of the Protective Effects of Hesperidin in Lipopolysaccharide-Induced Inflammation and Cytotoxicity of Cell
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(1) Background: Plant flavonoids are efficient in preventing and treating various diseases. This study aimed to evaluate the ability of hesperidin, a flavonoid found in citrus fruits, in inhibiting lipopolysaccharide (LPS) induced inflammation, which induced lethal toxicity in vivo, and to evaluate its importance as an antitumor agent in breast cancer. The in vivo experiments revealed the protective effects of hesperidin against the negative LPS effects on the liver and spleen of male mice. (2) Methods: In the liver, the antioxidant activity was measured by estimating the concentration of glutathione (GSH) and catalase (CAT), whereas in spleen, the concentration of cytokines including IL-33 and TNF-α was measured. The in vitro expe

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Publication Date
Mon Sep 30 2019
Journal Name
Environmental Engineering Research
Development of Bi-Langmuir model on the sorption of cadmium onto waste foundry sand: Effects of initial pH and temperature
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The present study develops the sorption model for simulating the effects of pH and temperature on the uptake of cadmium from contaminated water using waste foundry sand (WFS) by allowing the variation of the maximum adsorption capacity and affinity constant. The presence of two acidic functional groups with the same or different affinity is the basis in the derivation of the two models; Model 1 and Model 2 respectively. The developed Bi-Langmuir model with different affinity (Model 2) has a remarkable ability in the description of process under consideration with coefficient of determination > 0.9838 and sum of squared error < 0.08514. This result is proved by FTIR test where the weak acids responsible of cadmium ions removal

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Publication Date
Mon Oct 19 2026
Journal Name
Journal Of The College Of Basic Education
The impact of compound exercises in the development of some of the skills of the players football halls
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Publication Date
Wed Jan 30 2019
Journal Name
Journal Of The College Of Education For Women
The General Principles of The Curriculum In Some Manuscripts Arab And Muslim Scholars
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Our scientists Arabs old efforts in achieving texts that led to the foundations of general
rules they have set for this work in accordance with the systematic context is based on the
study, analysis and precision in dealing with this work, which became the basis for each
investigator to the present day, which was to them careful consideration manuscripts and
work to be closer to the image by its author, so produced their efforts in this area several
books provided the technical wizards of this science, which did not leave a gap in which only
gave her treatments and suggestions each and every one of the point made by and gave her
what produced the expertise in this direction in accordance with the formulations
meth

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Publication Date
Sat Nov 04 2023
Journal Name
Migration Letters
The Concept of Reform According to the Quranic Perspective
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This research is an initial attempt to explore the reality of reform and its concept in Islam, and to describe the presence of educational practices in the Arab-Islamic heritage. The importance of this research lies in addressing a number of important issues related to the contemporary Islamic reform system. The research showed the need for authentic educational reform values that draw their reference from the origins of Islam. The research also emphasized the analysis of the reality of education in the Muslim world and the identification of its problems and the search for solutions through a quick and comprehensive look at the diverse contributions of Muslim thinkers to Islamic educational reform thought, and their active role in addressin

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