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ISOLATION AND DIAGNOSIS OF SOME CANDIDA SPECIES FROM SOME BAGHDAD CITY HOSPITALS WITH PCR TECHNIQUE AND EVALUATION OFTHE EFFECTIVENESS OF SOME ANTIFUNGALS
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The current study aimed to isolate and diagnose Candida spp yeasts that cause candidiasis with a PCR device from patients reviewed for some hospitals in Baghdad city and by 190 samples, the study recorded 123 isolates and the total percentage of infection was 64.7% .Samples were taken from different clinical cases of the vagina, blood and mouth and the Candida spp were (70.37%, 41.26%, 86.95%) respectively. Five types of yeasts were isolated and diagnosed, namely C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C.glabarta. They were confirmed by PCR device and the most notable were yeast C. albicans, where 91 isolates were found, 73.98%, while the lowest infection was recorded. C.glabartawith 3 isolates, at 2.43%, significant differences at P  0.001.The C. albicans showed the ability to form a Grem tube and Chlamydospore formation. Cultivation on the differential medium, chromo agar, showed that the yeast of C. albicans in a light green color, C.tropicalis in a metallic blue and C.parapsilosis in a creamy white color as the C .krusei was light pink in color, while C.glabrata was pinkish-purple in color. Isolation and diagnosis of these species have been confirmed by the Vitek2 Compact System. Four types of antifungal agents, Nystatin, Amphotericin, Clotrimazole and Ketoconazole, were used, The results showed a different effect of antifungals on Candida spp. The results of the PCR using the fungi starter pair (ITS1, ITS4) showed that they produced different molecular sizes ranging from (510-870) bp where the type of C. albicans was 535 bp and C. krusie was bp 510 and C.tropicalis was bp 524 and C. parapsilosis was bp520 and C.glabrata were bp 870

Publication Date
Wed Feb 01 2023
Journal Name
Baghdad Science Journal
Synthesis, characterization and bioactivity Study from azo – ligand derived frommethyl-2-amino benzoatewith some metal ions
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A Ligand (ECA) methyl 2-((1-cyano-2-ethoxy-2-oxoethyl)diazenyl)benzoate with metals of (Co2+, Ni2+, Cu2+) were prepared and characterization using H-NMR, atomic absorption spectroscopy, ultra violet (UV) visible, magnetic moments measurements, bioactivity, and Molar conductivity measurements in soluble ethanol. Complexes have been prepared using a general formula which was suggested as [M (ECA)2] Cl2, where M = (Cobalt(II), Nickel(II) and Copper(II), the geometry shape of the complexes is octahedral.

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Publication Date
Mon Apr 23 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Antimicrobial activity of some plants extracts on bacteria isolated from acne vulgaris patients
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Background: Acne is a cutaneous pleomorphic disorder skin disease most frequently occurring during the adolescent in ages of 12-24, with estimated  percentage ( 85%) . There are different ways to treat acne such as  using of antibiotics  , herpes , and mixing treatments .

Methods : Antibacterial activity  of  four concentrations (100,50,25,12.5)mg /ml of  alcoholic  and cold  aqueous  crude extracts of Cinnamon(Cinnamomum verum ), Henna (Lawsonia inermis ) , Lupine (Lupinus luteus) were studied against aerobic and&nbs

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Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Synthesis & Characterization of Some 1, 3, 4-OxadiazoleDerivatives & new Cyclic Imides from Creatinine
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A series of new 1,3,4- oxadiazole derivatives and cyclic Imides containing Creatinine
heterocyclic molecule were prepared via following method:first step included Synthesis of
amic acid from reaction between different cyclic anhydrides with Creatinine which
subsequently dehydrated to the corresponding imide via treatment with acetic anhydride
and anhydrous sodium acetate,.then,second step was tratedamic acid with Thionyl chloride
producing acid chloride which on amination with hydrazine hydrate in thierd step and the
last turn to 1,3,4-oxadiazole derivatives by condensation reaction with Phosphorous
Oxychloride(POCl3) and different benzoic acids. Cyclization to 1, 3, 4-Oxadiazole
derivatives. Antibacterial activit

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Publication Date
Thu Mar 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
DNA Sequences of LasB Gene in Pseudomonas aeruginosa Isolated from Some Clinical Cases
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 Out of 120 isolates from different clinical cases, only 75 were found and confirmed that they belong to the Pseudomonas aeruginosa bacteria. The result revealed that the LasB virulent gene was present in 63 isolates with 63% percentage. The gel electrophoresis showed that the molecular weight of LasB gene was 300 bp. DNA sequences of LasB gene was done, and the results showed the presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotides sequence with the original sequence of amino acids revealed that there are no effects of gene mutations on translation of the product protein. 

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Publication Date
Sun Dec 29 2019
Journal Name
Iraqi Journal Of Science
Antibacterial activity of Some mouthwash Solutions against Staphylococcus lentus Isolated from Mouth Infections
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A total of nine swab samples were collected from inflamed teeth and gingiva of human’soral cavity from a dentist clinic in Baghdad. All specimens were cultured in Mitis Salivarius agar medium and the isolated bacterial pure colonies werethen identified by using VITEK2. Three samples were diagnosed and identified as Staphylococcus lentus. One of the three isolates which showed a distinctive heavy growth on the media was selected for further analysis in this study. Paper disk diffusion method was used to detect the antibacterial activityof three of mouthwash solutions (Zak, Colgate and Listerine). The results showed that “Colgate” was the most active solution with antibacterial activity compared with the other two soluti

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Publication Date
Mon Dec 30 2024
Journal Name
Iraqi Journal Of Science
Study of Corrosion Inhibition for Some New Schiff Bases Synthesized from Quinazolinone Derivative
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In this work, new Schiff bases of quinazolinone derivatives (Q1-Q5) were synthesized from methyl anthranilate. The synthesis involved three steps. In the first step, methyl anthranilate was reacted with isothiocyanatobenzene, producing the thiourea derivative K1. The second step entailed reacting K1 with hydrazine hydrate, synthesizing 3-amino-2-(phenylamino) quinazolin-4(3H)-one (K2). The third step involved reaction of K2 with various aromatic aldehydes, yielding the Schiff bases derivatives Q1-Q5. The chemical structures of these compounds were identified by FT-IR,1H NMR and 13C NMR spectroscopy. The newly synthesized derivatives (Q1-Q5) were subjected to rigorous evaluation to assess their efficacy as corrosion inhibitors for ca

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Publication Date
Wed Feb 01 2023
Journal Name
Archives Of Razi Institute
The Effect of Active Compounds and Trace Elements Extracted from Artemisia Fruit on Some Liver Enzymes in Humans
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Artemisia is a perennial wild shrub with large branches and compound leaves. Artemisia contains about 400 types, and its medical importance is due to the presence of many active substances and compounds such as volatile oils, alkaloids and flavonoids, glycosides, saponins, tannins, and coumarins. This study was designed to study the effect of the aqueous extract of the fruit of the Artemisia plant on the organs of the body, as well as to know its ability to activate the hepatic enzyme alanine transaminase (ALT/GPT). The fruit of this shrub was extracted using the measurement technique gas chromatography-mass spectrometry (GC/MASS) and organic solvent hexane and ethyl acetate in one to one ratio. It contained 21 compounds, a high percentage

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Scopus (2)
Scopus
Publication Date
Tue Jan 01 2019
Journal Name
African Journal Of Hospitality, Tourism And Leisure
Employing marketing information systems for the success of small and medium enterprises: A field study of some Tourism and Travel companies in Baghdad
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Scopus (6)
Scopus
Publication Date
Wed Apr 20 2022
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Evaluation the Effectiveness of Different Concentrations Phenols, Alkaloids and Terpenes Extracted from Pimpinella anisum against Phytophthora Fungi.
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This study did the isolation, purification, and identification of the fungus Phytophthora cinnamomi of some infected plants, including Chili pepper, cucumber, and eggplant. The green parts of Pimpinella anisum plant were grounded to a semi-powdered state. Phenols, alkaloids and terpenes were extracted from this plant, then the anti-fungal activity was evaluated at different concentrations of 5% and 10%. The percentage of radial growth inhibition of fungi with plant extracts was measured after seven days of incubation. The results showed that the terpene extract was the most effective against fungi and the alkaloid extract had the least antifungal activity. the percentage of radial growth inhibition was

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Publication Date
Sun Apr 30 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Synthesis and Study Biological Activity of Some New Isoxazoline and Pyrazoline derivatives
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   This work involves synthesis of novel Schiff bases derivatives contining isoxazoline or pyrazoline units starting with chalcons . 4-Aminoacetophenone was reacted with 3-nitrobenzaldehyde in basic medium giving chalcone [I] by claisen-schemidt reaction. The chalcone [I]  was reacted with hydroxylamine hydrochloride giving isoxazoline [II]  in basic medium. The chalcone [I] could also react with hydrazitne hydrate to give pyrazoline [III]  . The novel Schiff bases with structural formula [IV] and [V] were prepared by the reaction of amino compounds ; isoxazoline [II] and pyrazolines [III] with p-substituted aldehydes or p-subsituted ketones, respectively in dry benzene using drops of glacial acetic acid as a cat

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