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Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease
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Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chromatography using DEAE- cellulose A-50 . The results showed a single peak by using gel filtration and the activity reached 31-15 U/L .Two isoenzymes were obtained by using ion exchange chromatography and the purity degree of isoenzymse (I, II) were (5.7) and (5.53) fold respectively

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Publication Date
Sun Jul 03 2005
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Decrease of Serum AST, ALT, and GGT among Male Alcohol Drinkers with Coffee Consumption Habit.
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Summary:
Background: Alcohol is the most important causes of liver cirrhosis. Many of the factors underlying the development of alcoholic liver diseases remain unknown. Recently, some epidemiological studies showed beneficial effects of coffee against the occurrence of alcoholic liver cirrhosis and upon serum liver enzymes level. These observations have been examined in this work.
Patients and Methods: The relation of coffee drinking to serum GGT, AST and ALT activities were examined in 59 alcoholic male patients with or without habit of coffee consumption. 35 (59.3%) out of 59 patients were alcoholic drinkers without coffee consumption habit, and 24(40.7%) were alcohol drinkers with coffee consumption. In addition to 24 healthy pe

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Publication Date
Sun Jul 03 2005
Journal Name
Journal Of The Faculty Of Medicine Baghdad
HLA-DR and DQ Antigens in Iraqi Patients with Behget's Disease
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Background:

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Publication Date
Fri Jan 01 2010
Journal Name
Iraqi Journal Of Biotechnology
BIOCHEMICAL STUDY ON SUPEROXIDE DISMUTASE ENZYME IN PATIENTS WITH DIFFERENT BRAIN TUMORS
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The aim of the current study is to in evaluate the role of SOD activity in the previously reported oxidative stress in our laboratory(1), in the patients with different brain tumors. SOD activity was assayed according to riboflavin/NBT method and its specific activity was calculated in patients with benign and malignant brain tumors and control. Moreover the specific activity was compared in these samples according to gender and the occurrence of disease.Non significant elevation (P > 0.05) in SOD specific activity was observed in tissue of malignant tumors in comparison to that of in benign brain tumors. While a highly significant decrease (P < 0.001) of the specific activity was found in sera of malignant patients group in comparison to t

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Publication Date
Sun Jun 20 2021
Journal Name
Baghdad Science Journal
Hypothyroidism and Leptin in Iraqi Patients with Chronic Kidney Disease
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The present study aims at assessing the effects of chronic kidney disease (CKD) on thyroid hormone and leptin by evaluating the level of: leptin hormone along with thyroid hormone in CKD patients. The study has been conducted on  70 subjects, 50 patients with an age range between 20-50 years (25  males and 25 females) who were diagnosed to have CKD stage-5, and 20 normal controls whose ages ranged between 20-48 years (10  males and 10 females), who attended  the Nephrology and Transplant Center in Medical City of Baghdad- Iraq from April  2018  to July  2018. The study showed a highly significant (P<0.01) increase in TSH level in CKD patients in comparison with controls. While T3 and T4 levels observed highly significant decrea

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Publication Date
Tue Mar 30 2021
Journal Name
Baghdad Science Journal
Hypothyroidism and AMH in Iraqi Patients with Chronic Kidney Disease
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This study was conducted to investigate thyroid function and Anti-Müllerian hormone (AMH) in (Chronic kidney disease) CKD patients by evaluating their levels in CKD patients, 50 patients were diagnosed to have CKD stage-5, their ages ranged between 20-50 years (25 males and 25 females) who attended the Nephrology and Transplant Center in Medical City of Baghdad- Iraq, they were recruited from April 2018 to July 2018 and were enrolled into the study. The control group consisted of 20 healthy individuals, their ages ranged between 20-48 years (10 males and 10 females). The study showed non-significant (p>0.05) increase in AMH level in CKD patients compared to the control group. On the other hand, TSH was recorded a highly significant (

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Publication Date
Wed Jul 01 2009
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Prevalence of Fibromyalgia in Iraqi Patients with Ischemic Heart Disease
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Background: Fibromyalgia has been associated with physical and emotional trauma including invasive medical procedures. Both Fibromyalgia and ischemic heart disease have been linked with depression. The aim of the study is to assess the prevalence of fibromyalgia syndrome in patients with ischemic heart disease.Patients and Methods: A cross-sectional comparative study was done in the Iraqi Center for Heart Disease. One hundred patients, angiographically proved to have ischemic heart disease, were subjected to rheumatologic examination by another physician, and compared to hundred healthy individuals as a control group. Diagnosis of fibromyalgia was based on 1990 American College of Rheumatology Classification criteria for the diagnosis of

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Publication Date
Sun Jul 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
HLA prevalence in Iraqi patients with ischemic heart disease.
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Background: The etiology of ischemic heart disease (IHD) is believed to have an immunological component. Association with human leukocyte antigens (HLAs) has been previously reported, particularly with DR6.
Patients and methods: 75 cardiac patients were admitted to the coronary care unit, Baghdad Teaching Hospital over the period October 2008-May 2009 with the clinical diagnosis of acute
coronary syndrome and STEMI myocardial infarction their ages range was (25-82) years the number of male was (55) (73.3%) and female was (20) (26.7%). All cases have routine ECG, cardiac marker’s measurements, routine haematological, Biochemical test and 2mls of blood reserved for HLA study.
Results: It was found that H

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Evaluation of Some Immunological Markers in Patients with Behcet’s Disease
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The local study were selected 70 Iraqi Arab Patients (39 females and 31 males), who referred to HLA laboratory in teaching laboratories in Baghdad medical city from April 2009 – May 2010 were diagnosed with Behcete's disease, C-reactive protein concentration(CRP) was evaluated by ELISA technique, anti-nuclear antibody (ANA) and anti-neutrophil cytoplasmic antibody (ANCA) were evaluated by Indirect immunofluorescence technology. The result showed significant differences (P<0.05) of CRP concentration in patients group compared with control, negative antinuclear nuclear antibody in all cases while ANCA was positive in 8.5% of patients with type P (PANCA). These results lead to suggest that ANCA auto-antibody may have a role in triggeri

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Publication Date
Mon Aug 26 2019
Journal Name
Iraqi Journal Of Science
Seroprevalence of Toxoplasmosis in patients with chronic liver disease in Baghdad
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The influence of Toxoplasma gondii in the pathogenesis of hepatic disease has lately  had considerable attention. The objective of this study is to assess the seroprevalence of T. gondii infection in patients with chronic liver disease from Baghdad-Iraq. All patients have attended Gastroenterology and Hepatology  Teaching Hospital, Medical city in Baghdad, Iraq An analytical case–control study was achieved from September to November 2018. Seventy male patients with chronic liver disease (CLD) and 70 control males  (free of chronic liver diseases) participated in this study, their ages were between (18-80) years old. Serum samples were taken from all subjects and were analysed with enzyme-linked imm

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Publication Date
Sun Jul 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Salivary aspartate amino transferase and alanine amino transferase of non-insulin-dependents (Type2) diabetic patients.
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Background: Diabetes mellitus (DM) is a world-wide chronic metabolic disorder affects relatively high percentage of population. A few information is available in diabetes on the salivary content of enzymes GOT (AST) and GPT (ALT).
Patients and methods: we studied 19 patients (NIDDM, type 2 diabetes mellitus aged 29-67 year). Colorimetric determination of salivary GPT and GOT activity was used.
Results: In the whole saliva of non-insulin-dependent (NIDDM, n=19) diabetic patients and from agecross- matched healthy subjects (n=19). Saliva concentrations of GOT (43.92=12.8U/L) in NIDDM patients were higherly significant (P< 0.001) than those observed in normal subjects (23.0± 8.84 U/L). GPT was higher

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