The research was conducted in one of the fields of the Department of Plant Production in the desert region (tomato development project) affiliated with the Directorate of Agriculture of the Holy Karbala Governorate for the purpose of studying the effect of spraying the shoots of the Kohlrabi plant (hybrid) with the Nano biopolymer (Chitosan) and the Azotobacter bio inoculum on the roots of the plant seedlings in terms of growth indicators and yield for the autumn agricultural season. 2023-2024 ,The means were compared according to the Duncan multiple ranges test at significant to level of 0.05.with R.C.B.D . The first factor was spraying the shoots with Nano- Chitosan at a concentration of (0, 1, and 2) g L-1, two weeks after transplanting, and two sprays every 14 days. The second factor has three levels, the first is planting without a vaccine, the second is inoculating the seedling roots with Azotobacter at a level of 5 g , and the third level is inoculating with 10 grams of the biological factor. The results showed a significant superiority of the interaction treatment between the bio-addition of Azotobacter at a level of 5 g .and a concentration of 2 g of Nanochitosan in the characteristics of each of the plant length 60 cm, the number of leaves 21.67 leaves. plant-1the leaf content of chlorophyll 77.59 mg 100 g -1, The Weight of the knobs cm plant-1 278.9 g , the total yield 14.87 ton . h -1, content of nitrogen 3.43% and the knobs content of protein 21.48% compared to the control treatment, which gave the lowest value
