bstract         This study was performed to isolate and identify the Burkholderia cepacia from rhizosphers of different plants and study their ability to produce Siderophore, the study was also aimed to assess the antifungal activity of the bacterial filtrate and extracts of the isolates against some pathogenic fungi.     The isolate which showed higher inhibitory effects was selected for extraction of some active metabolites produced by it, and evaluate the activity in vitro, via inhibition of the growth of the fungi using different concentrations of extract (50, 100, 200 )µL/ml , The results showed that out of  (250) samples of soil , (8) isolates (3.2%) gave positive

From different hospitals in Baghdad city, 25 clinical isolates of Proteus spp. were collected from different clinical samples, all isolates were identified as Proteus mirabilis by using bacteriological and biochemical assays in addition to Vitek-2 identification system. 15 (60%) isolates were identifying as Proteus mirabilis. The susceptibility of P. mirabilis isolates towards cefotaxime and ceftazidime was (66.6 %), (20%) consecutively; while extended spectrum β-lactamases producing P. mirabilis percentage was (30.7 %). Because blaVEB-1 was documented as an important indicator for increasing risk of extended spectrum beta ßlactamases producing P. mirabilis isolates that began to spread from many geographic area to Far east which inc

A total of 200 clinical samples included Burns and Wounds infections were collected from Baghdad Governorate. Results showed that rate all isolates of P. mirabilis was 31(15.5%) and rate of Burns infections was 14 (45%) and rate of wounds infection 17 (55%). Where was diagnostic based on conventional biochemical tests and confirmed by the Vitek-2 Compact system and the specific primer of the16SrRNA gene, the ability of bacterial isolates to biofilm formation to be studied. It's considered an important virulence factor in Incidence of diseases and play important role in increasing resistance to antibiotic of encased bacteria, by two methods Congo Red Agar method and Microtiter Plate method. The Congo Red Agar method showed that most isolates

Abstract A total of 207 specimens were collected from different sources including patients, health care staff and hospital environment in Ibb city, Yemen. The study used the bacteriocin produced from active producer strains in typing of Staphylococcus aureus. Depending on the morphological, cultural and biochemical characteristics, 54 (26.09%) isolates of Staphylococcus aureus were identified. An antibiotic sensitivity test was done for the bacterial isolates, and the results showed that there were multiple resistant antibiotics. The Staphylococcin production of these isolates has been detected by using wells assay. Fifty one isolates were Staphylococcin producer. Four isolates (staph19, staph25, staph28 and staph43) were chosen as go

 Out of 120 isolates from different clinical cases, only 75 were found and confirmed that they belong to the Pseudomonas aeruginosa bacteria. The result revealed that the LasB virulent gene was present in 63 isolates with 63% percentage. The gel electrophoresis showed that the molecular weight of LasB gene was 300 bp. DNA sequences of LasB gene was done, and the results showed the presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotides sequence with the original sequence of amino acids revealed that there are no effects of gene mutations on translation of the product protein. 

This study was designed for isolation of some fungi that caused sub clinical mastitis in cow milk in AL-anbar governorate in Iraq . Total number of 100 milk samples were collected from 25 cow in AL- Falluja city , by using of California mastitis test 45% of milk samples were infected with sub clinical mastitis ,while 55% of milk samples were negative for California mastitis test. The results showed of fungal isolation of milk samples which were positive for California mastitis test, recording 19 isolates of different types of fungi : Aspergillus fumigatus2.2% , Aspergillus niger 4.4% , Candida albicans 26.6% , Blastomced dermetitidis% 4.4 and Geotrichum candidum4.4%. The isolation of these types of fungi, may be attributed to many factor

The functional properties of the defatted powder and protein isolate of germinated mung bean seeds were studied and the estimation of the amino acids was carried out. The results showed a significant increase in the values of the amino acids leucine, lysine, phenylalanine and valine. The results of studying the functional properties of the protein isolate and defatted powder showed that there were significant differences between the sample The defatted and protein isolate, if the water absorption capacity of the defatted mung powder was 2.5% water/gm protein and the water absorption capacity of the protein isolate was 3%ml water/g protein, the fat binding capacity of the defatted powder was 0.3 ml fat/g protein and the isolate The ratio was

This study was aimed to analysis phylogenetic tree of the gene cpn60 in Acinetobacter baumannii that was identified in Baghdad. Study included collection two hundred specimens (fifty from UTI, fifty from wound infection , fifty from respiratory tract infection and fifty from otitis infections) . In primary laboratory diagnosis and confirmed by using VITEK- 2 Compact system, twenty isolates of this bacterium were indentified (10%) from total specimens. Extraction of geneteic material to detect target gene by amplification this target gene. DNA
sequencing of all isolates was done. Then alignment of sequencing in NCBI and draw phylogenetic tree by use Geneious 9 software among sequence of locally i

This study includes isolation, purification, and identification of algae from different aquatic environments in Baghdad city. Nine unialgal cultures were obtained. These algal cultures included 6 species of blue-green algae (Microcystis aeruginosa, Microcystis flos-aquae, Oscillatoria limnetica, Nostoc carneum, Westillopesis prolifica, Mastigocluds lamiosus), and 3 species of green algae (Mougeotia sclaris, Scenedesmus dimorphus and Chlorella vulgaris). In addition that aerial parts from Convolvulus arvensis were collected. Terpens, alkaloids and phenols were extracted of mentioned plant, and the antialgal activity of extracts types were evaluated in 3 concentrations (5, 10, and 20 mg/ml) by wells and diffused in the agar media. Results

    The present study aimed to the isolation and identification of Penicillium chrysogenum from subclinical bovine mastitis as well as the evaluation of their potential to produce the main virulence factors by assessing proteinase production, urease production, growth rate at 37 ̊C, and hemolytic activity on Blood agar. One hundred milk samples were assembled from the White Gold village and surrounded outlying farms of Abu-Ghraib, Baghdad province, during the period from November 2018 to March 2019. Each milk sample was tested for California Mastitis (CMT). The results indicated that 85% of the samples gave positive (+ve) results for CMT. Sixty six mycotic isolates were detected, including 31 isolates of Peni